Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
240 results
Cited 19 times since 2015 (2.2 per year) source: EuropePMC
PloS one, Volume 10, Issue 8, 4 1 2015, Pages e0133888 Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications. Spronken MI, Short KR, Herfst S, Bestebroer TM, Vaes VP, van der Hoeven B, Koster AJ, Kremers GJ, Scott DP, Gultyaev AP, Sorell EM, de Graaf M, Bárcena M, Rimmelzwaan GF, Fouchier RA
Bioluminescent and fluorescent influenza A viruses offer new opportunities to study influenza virus replication, tropism and pathogenesis. To date, several influenza A reporter viruses have been described. These strategies typically focused on a single reporter gene (either bioluminescent or fluorescent) in a single virus backbone. However, whilst bioluminescence is suited to in vivo imaging, fluorescent viruses are more appropriate for microscopy. Therefore, the idea l reporter virus varies dep... Abstract
Cited 14 times since 2015 (1.6 per year) source: EuropePMC
Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology, Volume 23, Issue 1, 9 2 2015, Pages 24-36 Prognostic value of coronary computed tomography angiography in diabetic patients without chest pain syndrome. van den Hoogen IJ, de Graaf MA, Roos CJ, Leen AC, Kharagjitsingh AV, Wolterbeek R, Kroft LJ, Wouter Jukema J, Bax JJ, Scholte AJ
Aims: Diabetic patients with coronary artery disease (CAD) are often free of chest pain syndrome. A useful modality for non-invasive assessment of CAD is coronary computed tomography angiography (CTA). However, the prognostic value of CAD on coronary CTA in diabetic patients without chest pain syndrome is relatively unknown. Therefore, the aim was to investigate the long-term prognostic value of coronary CTA in a large population diabetic patients without chest pain syndrome. Methods: Between 20... Abstract
Cited 100 times since 2015 (11.3 per year) source: EuropePMC
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, Volume 20, Issue 26, 2 1 2015, Pages 21178 Emergence of a novel GII.17 norovirus – End of the GII.4 era? de Graaf M, van Beek J, Vennema H, Podkolzin AT, Hewitt J, Bucardo F, Templeton K, Mans J, Nordgren J, Reuter G, Lynch M, Rasmussen LD, Iritani N, Chan MC, Martella V, Ambert-Balay K, Vinjé J, White PA, Koopmans MP
In the winter of 2014/15 a novel GII.P17-GII.17 norovirus strain (GII.17 Kawasaki 2014) emerged, as a major cause of gastroenteritis outbreaks in China and Japan. Since their emergence these novel GII.P17-GII.17 viruses have replaced the previously dominant GII.4 genotype Sydney 2012 variant in some areas in Asia but were only detected in a limited number of cases on other continents. This perspective provides an overview of the available information on GII.17 viruses in order to gain insight in... Abstract
Cited 22 times since 2015 (2.4 per year) source: EuropePMC
Atherosclerosis, Volume 241, Issue 1, 1 1 2015, Pages 55-61 HDL cholesterol, leptin and interleukin-6 predict high risk coronary anatomy assessed by CT angiography in patients with stable chest pain. Caselli C, De Graaf MA, Lorenzoni V, Rovai D, Marinelli M, Del Ry S, Giannessi D, Bax JJ, Neglia D, Scholte AJ
Objectives: Coronary computed tomography angiography (CTA) describes several features of coronary plaques, i.e. location, severity, and composition. Integrated CTA scores are able to identify individual patterns of higher risk. We sought to test whether circulating biomarkers related with metabolism and inflammation could predict high risk coronary anatomy at CTA in patients with stable chest pain. Methods: We evaluated a panel of 17 biomarkers in 429 patients (60.3 ± 0.4 years, 268 males) with... Abstract
Cited 15 times since 2015 (1.7 per year) source: EuropePMC
The Journal of general virology, Volume 96, Issue 8, 22 4 2015, Pages 2050-2060 Influenza A virus evolution and spatio-temporal dynamics in Eurasian wild birds: a phylogenetic and phylogeographical study of whole-genome sequence data. Lewis NS, Verhagen JH, Javakhishvili Z, Russell CA, Lexmond P, Westgeest KB, Bestebroer TM, Halpin RA, Lin X, Ransier A, Fedorova NB, Stockwell TB, Latorre-Margalef N, Olsen B, Smith G, Bahl J, Wentworth DE, Waldenström J, Fouchier RAM, de Graaf M
Low pathogenic avian influenza A viruses (IAVs) have a natural host reservoir in wild waterbirds and the potential to spread to other host species. Here, we investigated the evolutionary, spatial and temporal dynamics of avian IAVs in Eurasian wild birds. We used whole-genome sequences collected as part of an intensive long-term Eurasian wild bird surveillance study, and combined this genetic data with temporal and spatial information to explore the virus evolutionary dynamics. Frequent reassort... Abstract
Cited 151 times since 2015 (16.4 per year) source: EuropePMC
Circulation. Cardiovascular imaging, Volume 8, Issue 3, 1 1 2015, Pages e002179 Detection of significant coronary artery disease by noninvasive anatomical and functional imaging. Neglia D, Rovai D, Caselli C, Pietila M, Teresinska A, Aguadé-Bruix S, Pizzi MN, Todiere G, Gimelli A, Schroeder S, Drosch T, Poddighe R, Casolo G, Anagnostopoulos C, Pugliese F, Rouzet F, Le Guludec D, Cappelli F, Valente S, Gensini GF, Zawaideh C, Capitanio S, Sambuceti G, Marsico F, Perrone Filardi P, Fernández-Golfín C, Rincón LM, Graner FP, de Graaf MA, Fiechter M, Stehli J, Gaemperli O, Reyes E, Nkomo S, Mäki M, Lorenzoni V, Turchetti G, Carpeggiani C, Marinelli M, Puzzuoli S, Mangione M, Marcheschi P, Mariani F, Giannessi D, Nekolla S, Lombardi M, Sicari R, Scholte AJ, Zamorano JL, Kaufmann PA, Underwood SR, Knuuti J, EVINCI Study Investigators
Background: The choice of imaging techniques in patients with suspected coronary artery disease (CAD) varies between countries, regions, and hospitals. This prospective, multicenter, comparative effectiveness study was designed to assess the relative accuracy of commonly used imaging techniques for identifying patients with significant CAD. Methods and results: A total of 475 patients with stable chest pain and intermediate likelihood of CAD underwent coronary computed tomographic angiography an... Abstract
Cited 25 times since 2015 (2.7 per year) source: EuropePMC
Journal of virological methods, Volume 217, 21 3 2015, Pages 55-63 Optimization of an enzyme-linked lectin assay suitable for rapid antigenic characterization of the neuraminidase of human influenza A(H3N2) viruses. Westgeest KB, Bestebroer TM, Spronken MI, Gao J, Couzens L, Osterhaus AD, Eichelberger M, Fouchier RA, de Graaf M
Antibodies to neuraminidase (NA), the second most abundant surface protein of the influenza virus, contribute to protection against influenza virus infection. Although traditional and miniaturized thiobarbituric acid (TBA) neuraminidase inhibition (NI) assays have been successfully used to characterize the antigenic properties of NA, these methods are cumbersome and not easily amendable to rapid screening. An additional difficulty of the NI assay is the interference by hemagglutinin (HA)-specifi... Abstract
Cited 3 times since 2015 (0.3 per year) source: EuropePMC
European heart journal. Cardiovascular Imaging, Volume 16, Issue 8, 13 2 2015, Pages 863-870 Changes in ischaemia as assessed with single-photon emission computed tomography myocardial perfusion imaging in high-risk patients with diabetes without cardiac symptoms: relation with coronary atherosclerosis on computed tomography coronary angiography. de Graaf MA, Roos CJ, Mansveld JM, Kharagjitsingh AV, Dibbets-Schneider P, Kroft LJ, Jukema JW, Ficaro EP, Bax JJ, Scholte AJ
Aims: The study aims (i) to evaluate changes in myocardial ischaemia on single-photon emission computed tomography (SPECT) myocardial perfusion imaging (MPI) after 2 years in a cohort of high-risk patients with diabetes without cardiac symptoms or known coronary artery disease (CAD) and (ii) to assess the value of baseline computed tomography coronary angiography (CTA)-derived coronary atherosclerosis parameters to predict changes in myocardial ischaemia. Methods and results: The population cons... Abstract
Cited 7 times since 2015 (0.8 per year) source: EuropePMC
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, Volume 48, Issue 5, 1 1 2015, Pages 724-30; discussion 730-1 Surgical treatment of aberrant aortic origin of coronary arteries. Kooij M, Vliegen HW, de Graaf MA, Hazekamp MG
Objectives: Aberrant origin of the coronary arteries is rare but can be life threatening. It is an important cause of sudden death in athletes and other young adults, and may be treated surgically. Consensus exists that interarterial left coronary artery (LCA) should be surgically repaired. For interarterial right coronary artery (RCA), the discussion remains open. The purpose of this study was to analyse our surgical experience. Methods: From 2001 until 2014, 31 patients were operated for inter... Abstract
Cited 3 times since 2015 (0.3 per year) source: EuropePMC
Cardiovascular diagnosis and therapy, Volume 5, Issue 1, 1 1 2015, Pages 63-66 Myocardial CT perfusion for the prediction of obstructive coronary artery disease, valuable or not? van Rosendael AR, de Graaf MA, Scholte AJ
Adenosine stress myocardial computed tomography perfusion (CTP) is a relatively new myocardial perfusion imaging technique. Together with coronary CT angiography (CTA) it provides anatomic and functional information of coronary artery disease (CAD). In previous studies, the combination of these techniques demonstrated to be valuable for identifying hemodynamically significant stenoses. George et al., performed a secondary analysis on the CORE320 study and compared the diagnostic performance of C... Abstract
Cited 3 times since 2015 (0.3 per year) source: EuropePMC
Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation, Volume 23, Issue 2, 1 1 2015, Pages 130-132 Cardiac arrest during vigorous exercise: coronary plaque rupture or myocardial ischaemia? van Rosendael AR, de Graaf MA, Scholte AJ
Cited 51 times since 2015 (5.5 per year) source: EuropePMC
Journal of virology, Volume 89, Issue 7, 21 3 2015, Pages 3763-3775 Identification of amino acid substitutions supporting antigenic change of influenza A(H1N1)pdm09 viruses. Koel BF, Mögling R, Chutinimitkul S, Fraaij PL, Burke DF, van der Vliet S, de Wit E, Bestebroer TM, Rimmelzwaan GF, Osterhaus AD, Smith DJ, Fouchier RA, de Graaf M
Unlabelled: The majority of currently circulating influenza A(H1N1) viruses are antigenically similar to the virus that caused the 2009 influenza pandemic. However, antigenic variants are expected to emerge as population immunity increases. Amino acid substitutions in the hemagglutinin protein can result in escape from neutralizing antibodies, affect viral fitness, and change receptor preference. In this study, we constructed mutants with substitutions in the hemagglutinin of A/Netherlands/602/0... Abstract
Cited 17 times since 2015 (1.8 per year) source: EuropePMC
Journal of the American Academy of Dermatology, Volume 72, Issue 3, 13 2 2015, Pages 465-472 Is cardiovascular evaluation necessary prior to and during beta-blocker therapy for infantile hemangiomas?: A cohort study. Raphael MF, Breugem CC, Vlasveld FA, de Graaf M, Slieker MG, Pasmans SG, Breur JM
Background: Although consensus guidelines for pretreatment evaluation and monitoring of propranolol therapy in patients with infantile hemangiomas (IH) have been formulated, little is known about the cardiovascular side effects. Objectives: We sought to analyze cardiovascular evaluations in patients with IH at baseline and during treatment with an oral beta-blocker. Methods: Data from 109 patients with IH were retrospectively analyzed. Patient and family history, pretreatment electrocardiogram (... Abstract
Cited 4 times since 2014 (0.4 per year) source: EuropePMC
PloS one, Volume 9, Issue 11, 12 2 2014, Pages e112129 Molecular epidemiology of seal parvovirus, 1988-2014. Bodewes R, Hapsari R, Rubio García A, Sánchez Contreras GJ, van de Bildt MW, de Graaf M, Kuiken T, Osterhaus AD
A novel parvovirus was discovered recently in the brain of a harbor seal (Phoca vitulina) with chronic meningo-encephalitis. Phylogenetic analysis of this virus indicated that it belongs to the genus Erythroparvovirus, to which also human parvovirus B19 belongs. In the present study, the prevalence, genetic diversity and clinical relevance of seal parvovirus (SePV) infections was evaluated in both harbor and grey seals (Halichoerus grypus) that lived in Northwestern European coastal waters from... Abstract
Cited 8 times since 2014 (0.8 per year) source: EuropePMC
Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions, Volume 85, Issue 3, 12 2 2014, Pages 480-487 Position of Edwards SAPIEN transcatheter valve in the aortic root in relation with the coronary ostia: implications for percutaneous coronary interventions. Katsanos S, Debonnaire P, van der Kley F, van Rosendael PJ, Joyce E, de Graaf MA, Schalij MJ, Scholte AJ, Bax JJ, Ajmone Marsan N, Delgado V
Objectives: To determine the implications of stable coverage of the coronary ostia by the Edwards SAPIEN valve frame in terms of myocardial ischemia and subsequent percutaneous coronary intervention (PCI), following transcatheter aortic valve implantation (TAVI). Background: Edwards SAPIEN frame is frequently deployed relatively higher than recommended and may overlap the coronary ostia. Methods: A total of 142 patients (age 81 ± 7 years, male 49%) treated with Edwards SAPIEN valve and with mult... Abstract
Cited 3 times since 2014 (0.3 per year) source: EuropePMC
JMIR research protocols, Volume 3, Issue 4, 3 1 2014, Pages e52 Treatment of Infantile Hemangioma in Regional Hospitals With eHealth Support: Evaluation of Feasibility and Acceptance by Parents and Doctors. de Graaf M, Totté JE, van Os-Medendorp H, van Renselaar W, Breugem CC, Pasmans SG
Background: Since beta blockers became the preferred treatment for infantile hemangiomas (IH), the number of patients eligible for treatment is increasing. Currently treatment of IH with beta blockers is mainly reserved for expert centers, where wait times are lengthening. This demonstrated the need for development of a more efficient and accessible way of providing care for children needing treatment for IH. An eHealth intervention, Hemangioma Treatment Plan (HTP), was developed to treat IH in... Abstract
Cited 6 times since 2014 (0.6 per year) source: EuropePMC
The American journal of cardiology, Volume 114, Issue 11, 16 3 2014, Pages 1646-1650 Relation between coronary arterial dominance and left ventricular ejection fraction after ST-segment elevation acute myocardial infarction in patients having percutaneous coronary intervention. Veltman CE, Hoogslag GE, Kharbanda RK, de Graaf MA, van Zwet EW, van der Hoeven BL, Delgado V, Bax JJ, Scholte AJ
The presence of a left dominant coronary artery system is associated with worse outcome after ST-segment elevation myocardial infarction (STEMI) compared with right dominance or a balanced coronary artery system. However, the association between coronary arterial dominance and left ventricular (LV) function at follow-up after STEMI is unclear. The present study aimed at evaluating the relation between coronary arterial dominance and LV ejection fraction (LVEF) shortly after STEMI and at 12-month... Abstract
Cited 6 times since 2014 (0.6 per year) source: EuropePMC
International journal of radiation oncology, biology, physics, Volume 90, Issue 1, 1 1 2014, Pages 164-171 Impact of cardiovascular counseling and screening in Hodgkin lymphoma survivors. Daniëls LA, Krol SD, de Graaf MA, Scholte AJ, van 't Veer MB, Putter H, de Roos A, Schalij MJ, van de Poll-Franse LV, Creutzberg CL
Purpose: Cardiovascular disease (CVD) is the most common nonmalignant cause of death in Hodgkin lymphoma (HL) survivors, especially after mediastinal irradiation. The role of screening for CVD in HL survivors is unclear, but confrontation with risks of CVD may have a negative influence on health-related quality of life (HRQL). As part of a phase 2 screening study using computed tomography angiography (CTA) among HL survivors, an HRQL analysis was done to evaluate the emotional and practical burd... Abstract
Cited 15 times since 2014 (1.5 per year) source: EuropePMC
The international journal of cardiovascular imaging, Volume 31, Issue 1, 27 4 2014, Pages 151-161 Automatic detection and quantification of the Agatston coronary artery calcium score on contrast computed tomography angiography. Ahmed W, de Graaf MA, Broersen A, Kitslaar PH, Oost E, Dijkstra J, Bax JJ, Reiber JH, Scholte AJ
Potentially, Agatston coronary artery calcium (CAC) score could be calculated on contrast computed tomography coronary angiography (CTA). This will make a separate non-contrast CT scan superfluous. This study aims to assess the performance of a novel fully automatic algorithm to detect and quantify the Agatston CAC score in contrast CTA images. From a clinical registry, 20 patients were randomly selected for each CAC category (i.e. 0, 1-99, 100-399, 400-999, ≥1,000). The Agatston CAC score on no... Abstract
Cited 21 times since 2014 (2.1 per year) source: EuropePMC
Journal of virology, Volume 88, Issue 17, 18 3 2014, Pages 9963-9975 Murine cytomegalovirus virion-associated protein M45 mediates rapid NF-κB activation after infection. Krause E, de Graaf M, Fliss PM, Dölken L, Brune W
Unlabelled: Murine cytomegalovirus (MCMV) rapidly induces activation of nuclear factor κB (NF-κB) upon infection of host cells. After a transient phase of activation, the MCMV M45 protein blocks all canonical NF-κB-activating pathways by inducing the degradation of the gamma subunit of the inhibitor of κB kinase complex (IKKγ; commonly referred to as the NF-κB essential modulator [NEMO]). Here we show that the viral M45 protein also mediates rapid NF-κB activation immediately after infection. MC... Abstract