Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
239 results
Cited 21 times since 2017 (2.9 per year) source: EuropePMC
Current opinion in virology, Volume 22, 2 1 2017, Pages 64-70 Capturing norovirus transmission. de Graaf M, Villabruna N, Koopmans MP
Human norovirus is a leading cause of gastroenteritis and is efficiently transmitted between humans and around the globe. The burden of norovirus infections in the global community and in health-care settings warrant the availability of outbreak prevention strategies and control measures that are tailored to the pathogen, outbreak setting and population at risk. A better understanding of viral and host determinants of transmission would aid in developing and fine-tuning such efforts. Here, we de... Abstract
Cited 12 times since 2017 (1.6 per year) source: EuropePMC
Emerging infectious diseases, Volume 23, Issue 1, 1 1 2017, Pages 87-91 Norovirus Infection in Harbor Porpoises. de Graaf M, Bodewes R, van Elk CE, van de Bildt M, Getu S, Aron GI, Verjans GM, Osterhaus AD, van den Brand JM, Kuiken T, Koopmans MP
A norovirus was detected in harbor porpoises, a previously unknown host for norovirus. This norovirus had low similarity to any known norovirus. Viral RNA was detected primarily in intestinal tissue, and specific serum antibodies were detected in 8 (24%) of 34 harbor porpoises from the North Sea. Abstract
Cited 22 times since 2016 (3 per year) source: EuropePMC
Current opinion in virology, Volume 22, 23 4 2016, Pages 1-6 Sustained fecal-oral human-to-human transmission following a zoonotic event. de Graaf M, Beck R, Caccio SM, Duim B, Fraaij P, Le Guyader FS, Lecuit M, Le Pendu J, de Wit E, Schultsz C
Bacterial, viral and parasitic zoonotic pathogens that transmit via the fecal-oral route have a major impact on global health. However, the mechanisms underlying the emergence of such pathogens from the animal reservoir and their persistence in the human population are poorly understood. Here, we present a framework of human-to-human transmission of zoonotic pathogens that considers the factors relevant for fecal-oral human-to-human transmission route at the levels of host, pathogen, and environ... Abstract
Cited 6 times since 2016 (0.8 per year) source: EuropePMC
The British journal of dermatology, Volume 175, Issue 6, 19 3 2016, Pages 1357-1360 Ulcerated infantile haemangiomas: the effect of the selective beta-blocker atenolol on wound healing. Ruitenberg G, Young-Afat DA, de Graaf M, Pasmans SG, Breugem CC
Cited 18 times since 2016 (2.3 per year) source: EuropePMC
The Journal of general virology, Volume 97, Issue 9, 30 5 2016, Pages 2255-2264 Comparison of norovirus genogroup I, II and IV seroprevalence among children in the Netherlands, 1963, 1983 and 2006. van Beek J, de Graaf M, Xia M, Jiang X, Vinjé J, Beersma M, de Bruin E, van de Vijver D, Holwerda M, van Houten M, Buisman AM, van Binnendijk R, Osterhaus ADME, van der Klis F, Vennema H, Koopmans MPG
Noroviruses are a major cause of acute gastroenteritis worldwide and are a genetically diverse group of viruses. Since 2002, an increasing number of norovirus outbreaks have been reported globally, but it is not clear whether this increase has been caused by a higher awareness or reflects the emergence of new genogroup II genotype 4 (GII.4) variants. The hypothesis that norovirus prevalence has increased post-2002 and is related to the emergence of GII.4 is tested in this study. Sera collected f... Abstract
Cited 33 times since 2016 (4.2 per year) source: EuropePMC
Journal of virology, Volume 90, Issue 13, 10 2 2016, Pages 5928-5938 Multiple Natural Substitutions in Avian Influenza A Virus PB2 Facilitate Efficient Replication in Human Cells. Mänz B, de Graaf M, Mögling R, Richard M, Bestebroer TM, Rimmelzwaan GF, Fouchier RAM
Unlabelled: A strong restriction of the avian influenza A virus polymerase in mammalian cells generally limits viral host-range switching. Although substitutions like E627K in the PB2 polymerase subunit can facilitate polymerase activity to allow replication in mammals, many human H5N1 and H7N9 viruses lack this adaptive substitution. Here, several previously unknown, naturally occurring, adaptive substitutions in PB2 were identified by bioinformatics, and their enhancing activity was verified u... Abstract
Cited 183 times since 2016 (23.1 per year) source: EuropePMC
Nature reviews. Microbiology, Volume 14, Issue 7, 23 4 2016, Pages 421-433 Human norovirus transmission and evolution in a changing world. de Graaf M, van Beek J, Koopmans MP
Norovirus infections are a major cause of gastroenteritis, and outbreaks occur frequently. Several factors are currently increasing the challenge posed by norovirus infections to global health, notably the increasing number of infections in immunocompromised individuals, who are more susceptible to disease, and the globalization of the food industry, which enables large norovirus outbreaks to occur on an international scale. Furthermore, the rapid rate of the genetic and antigenic evolution of c... Abstract
Cited 4 times since 2016 (0.5 per year) source: EuropePMC
The international journal of cardiovascular imaging, Volume 32, Issue 8, 21 3 2016, Pages 1311-1322 Automatic detection of aorto-femoral vessel trajectory from whole-body computed tomography angiography data sets. Gao X, Kitslaar PH, Budde RP, Tu S, de Graaf MA, Xu L, Xu B, Scholte AJ, Dijkstra J, Reiber JH
Extraction of the aorto-femoral vessel trajectory is important to utilize computed tomography angiography (CTA) in an integrated workflow of the image-guided work-up prior to trans-catheter aortic valve replacement (TAVR). The aim of this study was to develop a new, fully-automated technique for the extraction of the entire arterial access route from the femoral artery to the aortic root. An automatic vessel tracking algorithm was first used to find the centerline that connected the femoral acce... Abstract
Cited 6 times since 2016 (0.7 per year) source: EuropePMC
European heart journal. Cardiovascular Imaging, Volume 18, Issue 2, 24 4 2016, Pages 203-211 The influence of clinical and acquisition parameters on the interpretability of adenosine stress myocardial computed tomography perfusion. van Rosendael AR, de Graaf MA, Dimitriu-Leen AC, van Zwet EW, van den Hoogen IJ, Kharbanda RK, Bax JJ, Kroft LJ, Scholte AJ
Aims: The interpretation of adenosine stress myocardial computed tomography perfusion (CTP) is often hampered by image artefacts caused by cardiac motion, beam hardening, and cone beam. The aim of the present analysis was to assess the influence of the heart-rate response during adenosine infusion, patient characteristics, and medication use on the interpretability of stress myocardial CTP examinations. Methods and results: Interpretability of stress myocardial CTP examinations was evaluated in... Abstract
Cited 38 times since 2016 (4.7 per year) source: EuropePMC
European heart journal. Cardiovascular Imaging, Volume 17, Issue 9, 18 3 2016, Pages 951-960 Multicentre multi-device hybrid imaging study of coronary artery disease: results from the EValuation of INtegrated Cardiac Imaging for the Detection and Characterization of Ischaemic Heart Disease (EVINCI) hybrid imaging population. Liga R, Vontobel J, Rovai D, Marinelli M, Caselli C, Pietila M, Teresinska A, Aguadé-Bruix S, Pizzi MN, Todiere G, Gimelli A, Chiappino D, Marraccini P, Schroeder S, Drosch T, Poddighe R, Casolo G, Anagnostopoulos C, Pugliese F, Rouzet F, Le Guludec D, Cappelli F, Valente S, Gensini GF, Zawaideh C, Capitanio S, Sambuceti G, Marsico F, Filardi PP, Fernández-Golfín C, Rincón LM, Graner FP, de Graaf MA, Stehli J, Reyes E, Nkomo S, Mäki M, Lorenzoni V, Turchetti G, Carpeggiani C, Puzzuoli S, Mangione M, Marcheschi P, Giannessi D, Nekolla S, Lombardi M, Sicari R, Scholte AJ, Zamorano JL, Underwood SR, Knuuti J, Kaufmann PA, Neglia D, Gaemperli O, EVINCI Study Investigators
Aims: Hybrid imaging provides a non-invasive assessment of coronary anatomy and myocardial perfusion. We sought to evaluate the added clinical value of hybrid imaging in a multi-centre multi-vendor setting. Methods and results: Fourteen centres enrolled 252 patients with stable angina and intermediate (20-90%) pre-test likelihood of coronary artery disease (CAD) who underwent myocardial perfusion scintigraphy (MPS), CT coronary angiography (CTCA), and quantitative coronary angiography (QCA) with... Abstract
Cited 29 times since 2016 (3.5 per year) source: EuropePMC
Arteriosclerosis, thrombosis, and vascular biology, Volume 36, Issue 4, 11 2 2016, Pages 757-764 Effect of Coronary Atherosclerosis and Myocardial Ischemia on Plasma Levels of High-Sensitivity Troponin T and NT-proBNP in Patients With Stable Angina. Caselli C, Prontera C, Liga R, De Graaf MA, Gaemperli O, Lorenzoni V, Ragusa R, Marinelli M, Del Ry S, Rovai D, Giannessi D, Aguade-Bruix S, Clemente A, Bax JJ, Lombardi M, Sicari R, Zamorano J, Scholte AJ, Kaufmann PA, Knuuti J, Underwood SR, Clerico A, Neglia D
Objective: Circulating levels of high-sensitivity cardiac troponin T (hs-cTnT) and N terminal pro brain natriuretic peptide (NT-proBNP) are predictors of prognosis in patients with coronary artery disease (CAD). We aimed at evaluating the effect of coronary atherosclerosis and myocardial ischemia on cardiac release of hs-cTnT and NT-proBNP in patients with suspected CAD. Approach and results: Hs-cTnT and NT-proBNP were measured in 378 patients (60.1±0.5 years, 229 males) with stable angina and u... Abstract
Cited 10 times since 2016 (1.2 per year) source: EuropePMC
Heart (British Cardiac Society), Volume 102, Issue 12, 10 2 2016, Pages 943-949 Coronary anatomy as related to bicuspid aortic valve morphology. Koenraadt WM, Tokmaji G, DeRuiter MC, Vliegen HW, Scholte AJ, Siebelink HM, Gittenberger-de Groot AC, de Graaf MA, Wolterbeek R, Mulder BJ, Bouma BJ, Schalij MJ, Jongbloed MR
Objective: Variable coronary anatomy has been described in patients with bicuspid aortic valves (BAVs). This was never specified to BAV morphology, and prognostic relevance of coronary vessel dominance in this patient group is unclear. The purpose of this study was to evaluate valve morphology in relation to coronary artery anatomy and outcome in patients with isolated BAV and with associated aortic coarctation (CoA). Methods: Coronary anatomy was evaluated in 186 patients with BAV (141 men (79%... Abstract
Cited 10 times since 2016 (1.2 per year) source: EuropePMC
Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology, Volume 24, Issue 4, 9 2 2016, Pages 1253-1262 Relation between quantitative coronary CTA and myocardial ischemia by adenosine stress myocardial CT perfusion. van Rosendael AR, Kroft LJ, Broersen A, Dijkstra J, van den Hoogen IJ, van Zwet EW, Bax JJ, de Graaf MA, Scholte AJ
Background: Coronary-computed tomography angiography (CTA) has limited accuracy to predict myocardial ischemia. Besides luminal area stenosis, other coronary plaque morphology and composition parameters may help to assess ischemia. With the integration of coronary CTA and adenosine stress CT myocardial perfusion (CTP), reliable information regarding coronary anatomy and function can be derived in one procedure. This analysis aimed to investigate the association between coronary stenosis severity... Abstract
Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation, Volume 24, Issue 2, 1 1 2016, Pages 154-155 An anomalous left coronary artery with a malignant course: coronary angiography and myocardial perfusion imaging with computed tomography. de Graaf MA, van Rosendael AR, Kroft LJ, Vliegen HW, Hazekamp MG, Bax JJ, Scholte AJ
Cited 7 times since 2015 (0.8 per year) source: EuropePMC
The American journal of cardiology, Volume 117, Issue 6, 30 5 2016, Pages 887-893 Value of Coronary Computed Tomography Angiography in Tailoring Aspirin Therapy for Primary Prevention of Atherosclerotic Events in Patients at High Risk With Diabetes Mellitus. Dimitriu-Leen AC, Scholte AJ, van Rosendael AR, van den Hoogen IJ, Kharagjitsingh AV, Wolterbeek R, Knuuti J, Kroft LJ, Delgado V, Jukema JW, de Graaf MA, Bax JJ
Aspirin use for primary prevention in patients at high risk with diabetes mellitus (DM) is often recommended under the assumption that most patients with DM have coronary artery disease (CAD). However, not all patients may have CAD. The present study evaluated, in 425 patients at high risk with DM (without chest pain syndrome or a history of cardiac disease), the prevalence of CAD on coronary computed tomography angiography (CTA). Moreover, the association between the presence and number of trad... Abstract
Cited 12 times since 2015 (1.4 per year) source: EuropePMC
The American journal of cardiology, Volume 117, Issue 5, 14 2 2015, Pages 768-774 Prognostic Value of Coronary Computed Tomography Imaging in Patients at High Risk Without Symptoms of Coronary Artery Disease. Dedic A, Ten Kate GJ, Roos CJ, Neefjes LA, de Graaf MA, Spronk A, Delgado V, van Lennep JE, Moelker A, Ouhlous M, Scholte AJ, Boersma E, Sijbrands EJ, Nieman K, Bax JJ, de Feijter PJ
At present, traditional risk factors are used to guide cardiovascular management of asymptomatic subjects. Intensified surveillance may be warranted in those identified as high risk of developing cardiovascular disease (CVD). This study aims to determine the prognostic value of coronary computed tomography (CT) angiography (CCTA) next to the coronary artery calcium score (CACS) in patients at high CVD risk without symptoms suspect for coronary artery disease (CAD). A total of 665 patients at hig... Abstract
Cited 9 times since 2015 (1.1 per year) source: EuropePMC
The international journal of cardiovascular imaging, Volume 32, Issue 4, 14 2 2015, Pages 543-552 Enhanced characterization of calcified areas in intravascular ultrasound virtual histology images by quantification of the acoustic shadow: validation against computed tomography coronary angiography. Broersen A, de Graaf MA, Eggermont J, Wolterbeek R, Kitslaar PH, Dijkstra J, Bax JJ, Reiber JH, Scholte AJ
We enhance intravascular ultrasound virtual histology (VH) tissue characterization by fully automatic quantification of the acoustic shadow behind calcified plaque. VH is unable to characterize atherosclerosis located behind calcifications. In this study, the quantified acoustic shadows are considered calcified to approximate the real dense calcium (DC) plaque volume. In total, 57 patients with 108 coronary lesions were included. A novel post-processing step is applied on the VH images to quanti... Abstract
Cited 142 times since 2015 (16.7 per year) source: EuropePMC
Nature protocols, Volume 10, Issue 12, 29 5 2015, Pages 1939-1947 Human norovirus culture in B cells. Jones MK, Grau KR, Costantini V, Kolawole AO, de Graaf M, Freiden P, Graves CL, Koopmans M, Wallet SM, Tibbetts SA, Schultz-Cherry S, Wobus CE, Vinjé J, Karst SM
Human noroviruses (HuNoVs) are a leading cause of foodborne disease and severe childhood diarrhea, and they cause a majority of the gastroenteritis outbreaks worldwide. However, the development of effective and long-lasting HuNoV vaccines and therapeutics has been greatly hindered by their uncultivability. We recently demonstrated that a HuNoV replicates in human B cells, and that commensal bacteria serve as a cofactor for this infection. In this protocol, we provide detailed methods for culturi... Abstract
Cited 3 times since 2015 (0.3 per year) source: EuropePMC
Journal of fish biology, Volume 87, Issue 5, 19 3 2015, Pages 1191-1208 Shallow genetic divergence and species delineations in the endemic Labeobarbus species flock of Lake Tana, Ethiopia. Nagelkerke LA, Leon-Kloosterziel KM, Megens HJ, De Graaf M, Diekmann OE, Sibbing FA
To assess whether the species distinctions of Lake Tana's Labeobarbus spp. are supported by genetic information, microsatellite markers were used. A total of 376 Labeobarbus spp., belonging to 24 populations of 11 species from three regions of the lake (north, south and east), were sampled. Eight microsatellite markers were analysed. In general, differences between conspecific populations were smaller than differences between populations of different species. For six species, conspecific po... Abstract
Cited 19 times since 2015 (2.2 per year) source: EuropePMC
PloS one, Volume 10, Issue 8, 4 1 2015, Pages e0133888 Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications. Spronken MI, Short KR, Herfst S, Bestebroer TM, Vaes VP, van der Hoeven B, Koster AJ, Kremers GJ, Scott DP, Gultyaev AP, Sorell EM, de Graaf M, Bárcena M, Rimmelzwaan GF, Fouchier RA
Bioluminescent and fluorescent influenza A viruses offer new opportunities to study influenza virus replication, tropism and pathogenesis. To date, several influenza A reporter viruses have been described. These strategies typically focused on a single reporter gene (either bioluminescent or fluorescent) in a single virus backbone. However, whilst bioluminescence is suited to in vivo imaging, fluorescent viruses are more appropriate for microscopy. Therefore, the idea l reporter virus varies dep... Abstract