Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
239 results
Cited 31 times since 2014 (3.1 per year) source: EuropePMC
Future virology, Volume 9, Issue 5, 1 1 2014, Pages 513-524 Avian influenza A viruses: from zoonosis to pandemic. Richard M, de Graaf M, Herfst S
Zoonotic influenza A viruses originating from the animal reservoir pose a threat for humans, as they have the ability to trigger pandemics upon adaptation to and invasion of an immunologically naive population. Of particular concern are the H5N1 viruses that continue to circulate in poultry in numerous countries in Europe, Asia and Africa, and the recently emerged H7N9 viruses in China, due to their relatively high number of human fatalities and pandemic potential. To start a pandemic, zoonotic... Abstract
Cited 17 times since 2014 (1.7 per year) source: EuropePMC
The American journal of cardiology, Volume 113, Issue 12, 1 1 2014, Pages 1947-1955 Feasibility of an automated quantitative computed tomography angiography-derived risk score for risk stratification of patients with suspected coronary artery disease. de Graaf MA, Broersen A, Ahmed W, Kitslaar PH, Dijkstra J, Kroft LJ, Delgado V, Bax JJ, Reiber JH, Scholte AJ
Coronary computed tomography angiography (CTA) has important prognostic value. Additionally, quantitative CTA (QCT) provides a more detailed accurate assessment of coronary artery disease (CAD) on CTA. Potentially, a risk score incorporating all quantitative stenosis parameters allows accurate risk stratification. Therefore, the purpose of this study was to determine if an automatic quantitative assessment of CAD using QCT combined into a CTA risk score allows risk stratification of patients. In... Abstract
Cited 178 times since 2014 (17.7 per year) source: EuropePMC
Cell, Volume 157, Issue 2, 1 1 2014, Pages 329-339 Identification, characterization, and natural selection of mutations driving airborne transmission of A/H5N1 virus. Linster M, van Boheemen S, de Graaf M, Schrauwen EJA, Lexmond P, Mänz B, Bestebroer TM, Baumann J, van Riel D, Rimmelzwaan GF, Osterhaus ADME, Matrosovich M, Fouchier RAM, Herfst S
Recently, A/H5N1 influenza viruses were shown to acquire airborne transmissibility between ferrets upon targeted mutagenesis and virus passage. The critical genetic changes in airborne A/Indonesia/5/05 were not yet identified. Here, five substitutions proved to be sufficient to determine this airborne transmission phenotype. Substitutions in PB1 and PB2 collectively caused enhanced transcription and virus replication. One substitution increased HA thermostability and lowered the pH of membrane f... Abstract
Cited 19 times since 2014 (1.9 per year) source: EuropePMC
Annals of oncology : official journal of the European Society for Medical Oncology, Volume 25, Issue 6, 31 5 2014, Pages 1198-1203 Screening for coronary artery disease after mediastinal irradiation in Hodgkin lymphoma survivors: phase II study of indication and acceptance†. Daniëls LA, Krol AD, de Graaf MA, Scholte AJ, Van't Veer MB, Putter H, de Roos A, Schalij MJ, Creutzberg CL
Background: Cardiovascular diseases are the most common nonmalignant cause of death in Hodgkin lymphoma (HL) survivors, especially after mediastinal irradiation. We investigated the role of computed tomographic coronary angiography (CTA) as a screening tool for coronary artery disease (CAD) in asymptomatic HL survivors, and related CTA findings to exercise testing and subsequent interventions. Patients and methods: Patients were eligible for this phase II study if at least 10 years disease-free... Abstract
Cited 235 times since 2014 (23.3 per year) source: EuropePMC
The EMBO journal, Volume 33, Issue 8, 25 4 2014, Pages 823-841 Role of receptor binding specificity in influenza A virus transmission and pathogenesis. de Graaf M, Fouchier RA
The recent emergence of a novel avian A/H7N9 influenza virus in poultry and humans in China, as well as laboratory studies on adaptation and transmission of avian A/H5N1 influenza viruses, has shed new light on influenza virus adaptation to mammals. One of the biological traits required for animal influenza viruses to cross the species barrier that received considerable attention in animal model studies, in vitro assays, and structural analyses is receptor binding specificity. Sialylated glycans... Abstract
Cited 11 times since 2014 (1.1 per year) source: EuropePMC
Veterinary microbiology, Volume 170, Issue 3-4, 2 1 2014, Pages 266-277 Phylogeny of Spanish swine influenza viruses isolated from respiratory disease outbreaks and evolution of swine influenza virus within an endemically infected farm. Martín-Valls GE, Simon-Grifé M, van Boheemen S, de Graaf M, Bestebroer TM, Busquets N, Martín M, Casal J, Fouchier RA, Mateu E
In the present study, outbreaks of respiratory disease were investigated for the presence of swine influenza virus (SIV). In 14 cases the circulating SIV strains were isolated, fully sequenced and compared with other known SIVs. The viruses causing the outbreaks belonged to the H1N1 (including human pandemic H1N1), H3N2 and H1N2 subtypes. In 11/14 cases the phylogenetic analyses indicated the occurrence of probable reassortment events. In the second part of the study, the genetic evolution of H1... Abstract
Cited 6 times since 2014 (0.6 per year) source: EuropePMC
Journal of the American Academy of Dermatology, Volume 70, Issue 5, 10 2 2014, Pages 893-898 E-learning enables parents to assess an infantile hemangioma. de Graaf M, Knol MJ, Totté JE, van Os-Medendorp H, Breugem CC, Pasmans SG
Background: Infantile hemangiomas (IH) at risk for complications need to be recognized early. Objective: We sought to determine if parents are able to assess, after e-learning, whether their child has an IH, is at risk for complications, and needs to be seen (urgently) by a specialist. Methods: This was a prospective study of 158 parents participating in an IH e-learning module. Parents were asked to assess their child's skin abnormality. A dermatologist answered the same questions (by e-co... Abstract
Cited 1 times since 2014 (0.1 per year) source: EuropePMC
Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation, Volume 22, Issue 2, 1 1 2014, Pages 77-79 ST-segment elevation associated with allergic reaction to echocardiographic contrast agent administration. van Ginkel A, Sorgdrager B, de Graaf MA, Karalis I, Ajmone Marsan N
We report a case of an allergic reaction after the administration of an echocardiographic contrast agent which resulted in ST-segment elevation. Hypersensitivity and allergic reactions are known causes of acute cardiovascular events. However, only limited reports are available which suggest the exact mechanism of the occurrence of angina or myocardial infarction during severe allergic reactions. In our case, through invasive imaging (coronary angiography and IVUS) we have shown for the first tim... Abstract
Cited 15 times since 2014 (1.5 per year) source: EuropePMC
Journal of virology, Volume 88, Issue 8, 29 5 2014, Pages 4595-4599 Novel avian-origin influenza A (H7N9) virus attachment to the respiratory tract of five animal models. Siegers JY, Short KR, Leijten LM, de Graaf M, Spronken MI, Schrauwen EJ, Marshall N, Lowen AC, Gabriel G, Osterhaus AD, Kuiken T, van Riel D
We determined the pattern of attachment of the avian-origin H7N9 influenza viruses A/Anhui/1/2013 and A/Shanghai/1/2013 to the respiratory tract in ferrets, macaques, mice, pigs, and guinea pigs and compared it to that in humans. The H7N9 attachment pattern in macaques, mice, and to a lesser extent pigs and guinea pigs resembled that in humans more closely than the attachment pattern in ferrets. This information contributes to our knowledge of the different animal models for influenza. Abstract
Cited 95 times since 2013 (9.2 per year) source: EuropePMC
Journal of virology, Volume 88, Issue 5, 26 4 2013, Pages 2844-2857 Genomewide analysis of reassortment and evolution of human influenza A(H3N2) viruses circulating between 1968 and 2011. Westgeest KB, Russell CA, Lin X, Spronken MI, Bestebroer TM, Bahl J, van Beek R, Skepner E, Halpin RA, de Jong JC, Rimmelzwaan GF, Osterhaus AD, Smith DJ, Wentworth DE, Fouchier RA, de Graaf M
Unlabelled: Influenza A(H3N2) viruses became widespread in humans during the 1968 H3N2 virus pandemic and have been a major cause of influenza epidemics ever since. These viruses evolve continuously by reassortment and genomic evolution. Antigenic drift is the cause for the need to update influenza vaccines frequently. Using two data sets that span the entire period of circulation of human influenza A(H3N2) viruses, it was shown that influenza A(H3N2) virus evolution can be mapped to 13 antigeni... Abstract
Cited 12 times since 2013 (1.2 per year) source: EuropePMC
JMIR research protocols, Volume 2, Issue 2, 17 3 2013, Pages e54 Evaluation of the Compliance, Acceptance, and Usability of a Web-Based eHealth Intervention for Parents of Children With Infantile Hemangiomas: Usability Study. de Graaf M, Totte J, Breugem C, van Os-Medendorp H, Pasmans S
Background: Infantile hemangiomas (IH) are common benign vascular tumors in children. Recognition and timely referral of high risk IH to specialized centers is important. This might be achieved by involving parents in the care for IH by means of an eHealth intervention. Objective: The objective of our study was to evaluate parent compliance, acceptance, and usability of an open access, Web-based eHealth intervention (including e-learning and e-consult) designed to increase parents' knowledg... Abstract
Cited 9 times since 2013 (0.9 per year) source: EuropePMC
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Volume 55, Issue 1, 12 2 2013, Pages 50-57 Additional diagnostic value of integrated analysis of cardiac CTA and SPECT MPI using the SMARTVis system in patients with suspected coronary artery disease. Kirişli HA, Gupta V, Shahzad R, Al Younis I, Dharampal A, Geuns RJ, Scholte AJ, de Graaf MA, Joemai RM, Nieman K, van Vliet L, van Walsum T, Lelieveldt B, Niessen WJ
Unlabelled: CT angiography (CTA) and SPECT myocardial perfusion imaging (MPI) are complementary imaging techniques to assess coronary artery disease (CAD). Spatial integration and combined visualization of SPECT MPI and CTA data may facilitate correlation of myocardial perfusion defects and subtending coronary arteries and thus offer additional diagnostic value over either stand-alone or side-by-side interpretation of the respective datasets from the 2 modalities. In this study, we investigated... Abstract
Cited 3 times since 2013 (0.3 per year) source: EuropePMC
The American journal of cardiology, Volume 113, Issue 5, 12 2 2013, Pages 772-778 Prognostic value of aortic and mitral valve calcium detected by contrast cardiac computed tomography angiography in patients with suspicion of coronary artery disease. Kamperidis V, de Graaf MA, Broersen A, Ahmed W, Sianos G, Delgado V, Dijkstra J, Bax JJ, Scholte AJ
Aortic valve calcium (VC) detected on noncontrast cardiac computed tomography angiography (CCTA) is known to be associated with all-cause mortality in asymptomatic and primary prevention population. However, the clinical significance of aortic and mitral VC remains unknown in symptomatic patients with suspected coronary artery disease (CAD). The aim of the present study was to assess whether aortic and mitral VC is independently associated with cardiac events and all-cause mortality in symptomat... Abstract
Cited 1 times since 2013 (0.1 per year) source: EuropePMC
Journal of plastic, reconstructive & aesthetic surgery : JPRAS, Volume 67, Issue 3, 27 4 2013, Pages 411-412 Response from the authors of 'Treatment of infantile haemangiomas with atenolol: comparison with a historical propranolol group'. de Graaf M, Raphael MF, Breugem CC, Knol MJ, Bruijnzeel-Koomen CA, Kon M, Breur JM, Pasmans SG
Cited 47 times since 2013 (4.4 per year) source: EuropePMC
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Volume 33, Issue 4, 29 5 2013, Pages 479-490 Determinants of virulence of influenza A virus. Schrauwen EJ, de Graaf M, Herfst S, Rimmelzwaan GF, Osterhaus AD, Fouchier RA
Influenza A viruses cause yearly seasonal epidemics and occasional global pandemics in humans. In the last century, four human influenza A virus pandemics have occurred. Occasionally, influenza A viruses that circulate in other species cross the species barrier and infect humans. Virus reassortment (i.e. mixing of gene segments of multiple viruses) and the accumulation of mutations contribute to the emergence of new influenza A virus variants. Fortunately, most of these variants do not have the... Abstract
Cited 39 times since 2013 (3.7 per year) source: EuropePMC
The American journal of pathology, Volume 183, Issue 4, 10 2 2013, Pages 1137-1143 Novel avian-origin influenza A (H7N9) virus attaches to epithelium in both upper and lower respiratory tract of humans. van Riel D, Leijten LME, de Graaf M, Siegers JY, Short KR, Spronken MIJ, Schrauwen EJA, Fouchier RAM, Osterhaus ADME, Kuiken T
Influenza A viruses from animal reservoirs have the capacity to adapt to humans and cause influenza pandemics. The occurrence of an influenza pandemic requires efficient virus transmission among humans, which is associated with virus attachment to the upper respiratory tract. Pandemic severity depends on virus ability to cause pneumonia, which is associated with virus attachment to the lower respiratory tract. Recently, a novel avian-origin H7N9 influenza A virus with unknown pandemic potential... Abstract
Cited 30 times since 2013 (2.8 per year) source: EuropePMC
Journal of plastic, reconstructive & aesthetic surgery : JPRAS, Volume 66, Issue 12, 4 1 2013, Pages 1732-1740 Treatment of infantile haemangiomas with atenolol: comparison with a historical propranolol group. de Graaf M, Raphael MF, Breugem CC, Knol MJ, Bruijnzeel-Koomen CA, Kon M, Breur JM, Pasmans SG
Propranolol, a lipophilic non-selective beta-blocker, has proven to be effective in the treatment of infantile haemangioma (IH). However, several side effects have been reported. Atenolol, a hydrophilic selective beta-1 blocker, could be an alternative and associated with fewer side effects. Thirty consecutive patients with IH were treated with atenolol between June 2010 and May 2011. The therapeutic effect was judged by clinical assessment and quantified by using a visual analogue scale (VAS) a... Abstract
Cited 2 times since 2013 (0.2 per year) source: EuropePMC
European heart journal, Volume 34, Issue 41, 21 3 2013, Pages 3168-3170 High coronary plaque load: a heavy burden. de Graaf MA, Jukema JW
Cited 46 times since 2013 (4.3 per year) source: EuropePMC
Virus research, Volume 178, Issue 1, 13 2 2013, Pages 15-20 Transmission of influenza A/H5N1 viruses in mammals. Imai M, Herfst S, Sorrell EM, Schrauwen EJ, Linster M, De Graaf M, Fouchier RA, Kawaoka Y
Highly pathogenic avian H5N1 influenza A viruses occasionally infect humans and cause severe respiratory disease and fatalities. Currently, these viruses are not efficiently transmitted from person to person, although limited human-to-human transmission may have occurred. Nevertheless, further adaptation of avian H5N1 influenza A viruses to humans and/or reassortment with human influenza A viruses may result in aerosol transmissible viruses with pandemic potential. Although the full range of fac... Abstract
Cited 142 times since 2013 (13.2 per year) source: EuropePMC
Nature, Volume 501, Issue 7468, 7 1 2013, Pages 560-563 Limited airborne transmission of H7N9 influenza A virus between ferrets. Richard M, Schrauwen EJ, de Graaf M, Bestebroer TM, Spronken MI, van Boheemen S, de Meulder D, Lexmond P, Linster M, Herfst S, Smith DJ, van den Brand JM, Burke DF, Kuiken T, Rimmelzwaan GF, Osterhaus AD, Fouchier RA
Wild waterfowl form the main reservoir of influenza A viruses, from which transmission occurs directly or indirectly to various secondary hosts, including humans. Direct avian-to-human transmission has been observed for viruses of subtypes A(H5N1), A(H7N2), A(H7N3), A(H7N7), A(H9N2) and A(H10N7) upon human exposure to poultry, but a lack of sustained human-to-human transmission has prevented these viruses from causing new pandemics. Recently, avian A(H7N9) viruses were transmitted to humans, cau... Abstract