Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
140 results
Cited 21 times since 2019 (4 per year) source: EuropePMC
PLoS biology, Volume 17, Issue 1, 29 5 2019, Pages e2006767 The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7. Köhler K, Sanchez-Pulido L, Höfer V, Marko A, Ponting CP, Snijders AP, Feederle R, Schepers A, Boos D
Accurate genome duplication underlies genetic homeostasis. Metazoan Mdm2 binding protein (MTBP) forms a main regulatory platform for origin firing together with Treslin/TICRR and TopBP1 (Topoisomerase II binding protein 1 (TopBP1)-interacting replication stimulating protein/TopBP1-interacting checkpoint and replication regulator). We report the first comprehensive analysis of MTBP and reveal conserved and metazoa-specific MTBP functions in replication. This suggests that metazoa have evolved spe... Abstract
Cited 14 times since 2019 (2.6 per year) source: EuropePMC
Endocrine-related cancer, Volume 26, Issue 1, 1 1 2019, Pages 1-12 Hotspot DAXX, PTCH2 and CYFIP2 mutations in pancreatic neuroendocrine neoplasms. Vandamme T, Beyens M, Boons G, Schepers A, Kamp K, Biermann K, Pauwels P, De Herder WW, Hofland LJ, Peeters M, Van Camp G, Op de Beeck K
Mutations in DAXX/ATRX, MEN1 and genes involved in the phosphoinositide-3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway have been implicated in pancreatic neuroendocrine neoplasms (pNENs). However, mainly mutations present in the majority of tumor cells have been identified, while proliferation-driving mutations could be present only in small fractions of the tumor. This study aims to identify high- and low-abundance mutations in pNENs using ultra-deep targeted resequencing. F... Abstract
Cited 10 times since 2018 (1.8 per year) source: EuropePMC
Langenbeck's archives of surgery, Volume 403, Issue 8, 10 2 2018, Pages 999-1005 Safety and efficacy of subtotal or total parathyroidectomy for patients with secondary or tertiary hyperparathyroidism in four academic centers in the Netherlands. van der Plas WY, Dulfer RR, Koh EY, Vogt L, Appelman-Dijkstra NM, Schepers A, Rotmans JI, Pol RA, van Ginhoven TM, Hoorn EJ, Nieveen van Dijkum EJM, Engelsman AF, de Borst MH, Kruijff S, Dutch Hyperparathyroidism Study Group (DHSG)
Purpose: Hyperparathyroidism (HPT) is a common abnormality in patients with end-stage renal disease (ESRD). Since the introduction of cinacalcet in 2004, a shift from surgery toward predominantly medical treatment has occurred. Surgery is thought to be associated with more complications than oral medication. The aim of this retrospective study was to evaluate 30-day outcomes and effectiveness of parathyroidectomy (PTx) in ESRD patients in the Netherlands. Methods: A national database containing... Abstract
Cited 2 times since 2018 (0.4 per year) source: EuropePMC
Annals of surgery, Volume 268, Issue 5, 1 1 2018, Pages 756-761 No Clinical Benefit of Intramuscular Delivery of Bone Marrow-derived Mononuclear Cells in Nonreconstructable Peripheral Arterial Disease: Results of a Phase-III Randomized-controlled Trial. Lindeman JHN, Zwaginga JJ, Kallenberg-Lantrua G, van Wissen RC, Schepers A, van Bockel HJ, Fibbe WE, Hamming JF
Background and aims: Prospects for no-option, end-stage peripheral artery disease (PAD) patients remain poor. Although results from open and semiblinded studies fuel hope for cell-based strategies in no-option patients, so far conclusions from the available placebo-controlled studies are not supportive. With the intention to end the remaining controversy with regard to cell therapy for PAD we conducted a confirmatory, double-blinded randomized placebo-controlled phase 3 trial. Study design: This... Abstract
Cited 11 times since 2018 (2 per year) source: EuropePMC
Genetics in medicine : official journal of the American College of Medical Genetics, Volume 21, Issue 5, 5 1 2018, Pages 1199-1208 Variants affecting diverse domains of MEPE are associated with two distinct bone disorders, a craniofacial bone defect and otosclerosis. Schrauwen I, Valgaeren H, Tomas-Roca L, Sommen M, Altunoglu U, Wesdorp M, Beyens M, Fransen E, Nasir A, Vandeweyer G, Schepers A, Rahmoun M, van Beusekom E, Huentelman MJ, Offeciers E, Dhooghe I, Huber A, Van de Heyning P, Zanetti D, De Leenheer EMR, Gilissen C, Hoischen A, Cremers CW, Verbist B, de Brouwer APM, Padberg GW, Pennings R, Kayserili H, Kremer H, Van Camp G, van Bokhoven H
Purpose: To characterize new molecular factors implicated in a hereditary congenital facial paresis (HCFP) family and otosclerosis. Methods: We performed exome sequencing in a four-generation family presenting nonprogressive HCFP and mixed hearing loss (HL). MEPE was analyzed using either Sanger sequencing or molecular inversion probes combined with massive parallel sequencing in 89 otosclerosis families, 1604 unrelated affected subjects, and 1538 unscreened controls. Results: Exome sequencing i... Abstract
Cited 31 times since 2018 (5.4 per year) source: EuropePMC
Journal of internal medicine, Volume 285, Issue 1, 7 1 2018, Pages 59-74 Blockade of vascular endothelial growth factor receptor 2 inhibits intraplaque haemorrhage by normalization of plaque neovessels. de Vries MR, Parma L, Peters HAB, Schepers A, Hamming JF, Jukema JW, Goumans MJTH, Guo L, Finn AV, Virmani R, Ozaki CK, Quax PHA
Background: Plaque angiogenesis is associated with atherosclerotic lesion growth, plaque instability and negative clinical outcome. Plaque angiogenesis is a natural occurring process to fulfil the increasing demand of oxygen and nourishment of the vessel wall. However, inadequate formed, immature plaque neovessels are leaky and cause intraplaque haemorrhage. Objective: Blockade of VEGFR2 normalizes the unbridled process of plaque neovessel formation and induces maturation of nascent vessels resu... Abstract
Cited 6 times since 2018 (1 per year) source: EuropePMC
Endocrine, Volume 62, Issue 3, 20 3 2018, Pages 639-647 SSTR2A expression in medullary thyroid carcinoma is correlated with longer survival. de Vries LH, Lodewijk L, Willems SM, Dreijerink KMA, de Keizer B, van Diest PJ, Schepers A, Bonenkamp HJ, van Engen-van Grunsven IACH, Kruijff S, van Hemel BM, Links TP, Nieveen van Dijkum EJM, van Eeden S, Valk GD, Borel Rinkes IHM, Vriens MR
Purpose: Medullary thyroid carcinoma (MTC) derives from the parafollicular C-cells of the thyroid gland. Somatostatin receptors (SSTRs) are expressed in various neuroendocrine tumours including MTC. The aim of this study was to evaluate SSTR2A as a prognostic factor for MTC, to study distribution of SSTR2A expression within tumours and to compare expression of SSTR2A between primary tumours and corresponding lymph node metastases. Methods: Patients who underwent surgery between 1988 and 2014 for... Abstract
Accident; analysis and prevention, Volume 125, 18 3 2018, Pages 336-343 Forecasting German crash numbers: The effect of meteorological variables. Diependaele K, Martensen H, Lerner M, Schepers A, Bijleveld F, Commandeur JJF
At the end of each year, the German Federal Highway Research Institute (BASt) publishes the road safety balance of the closing year. They describe the development of accident and casualty numbers disaggregated by road user types, age groups, type of road and the consequences of the accidents. However, at the time of publishing, these series are only available for the first eight or nine months of the year. To make the balance for the whole year, the last three or four months are forecasted. The... Abstract
Cited 8 times since 2018 (1.4 per year) source: EuropePMC
Human pathology, Volume 81, 25 4 2018, Pages 245-254 The theranostic target prostate-specific membrane antigen is expressed in medullary thyroid cancer. Lodewijk L, Willems SM, Dreijerink KMA, de Keizer B, van Diest PJ, Schepers A, Morreau H, Bonenkamp HJ, Van Engen-van Grunsven IACH, Kruijff S, van Hemel BM, Links TP, Nieveen van Dijkum E, van Eeden S, Valk GD, Borel Rinkes IHM, Vriens MR
Cited 3 times since 2018 (0.5 per year) source: EuropePMC
Journal of pediatric orthopedics, Volume 38, Issue 5, 1 1 2018, Pages 260-265 Prognostication in Developmental Dysplasia of the Hip Using the Ossific Nucleus Center Edge Angle. Firth GB, Robertson AJF, Ramguthy Y, Ramachandran M, Schepers A
Background: The aim of this paper was to determine the prognostic potential of the ossific nucleus center edge angle (ONCEA) in patients below 5 years of age treated for developmental dysplasia of the hip (DDH) to predict final outcome and the need for a secondary procedure. Methods: The interobserver and intraobserver reliability was calculated using the intraclass correlation coefficient for measurement of the ONCEA. The ONCEA was divided a priori into 3 groups: group A≥10 degrees, group B -9... Abstract
Cited 28 times since 2018 (4.6 per year) source: EuropePMC
Endocrine reviews, Volume 39, Issue 2, 1 1 2018, Pages 154-191 Diagnostic Utility of Molecular and Imaging Biomarkers in Cytological Indeterminate Thyroid Nodules. de Koster EJ, de Geus-Oei LF, Dekkers OM, van Engen-van Grunsven I, Hamming J, Corssmit EPM, Morreau H, Schepers A, Smit J, Oyen WJG, Vriens D
Indeterminate thyroid cytology (Bethesda III and IV) corresponds to follicular-patterned benign and malignant lesions, which are particularly difficult to differentiate on cytology alone. As ~25% of these nodules harbor malignancy, diagnostic hemithyroidectomy is still custom. However, advanced preoperative diagnostics are rapidly evolving.This review provides an overview of additional molecular and imaging diagnostics for indeterminate thyroid nodules in a preoperative clinical setting, includi... Abstract
Cited 53 times since 2018 (8.6 per year) source: EuropePMC
Circulation research, Volume 122, Issue 12, 15 3 2018, Pages 1648-1660 The COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking. Fedoseienko A, Wijers M, Wolters JC, Dekker D, Smit M, Huijkman N, Kloosterhuis N, Klug H, Schepers A, Willems van Dijk K, Levels JHM, Billadeau DD, Hofker MH, van Deursen J, Westerterp M, Burstein E, Kuivenhoven JA, van de Sluis B
Rationale: COMMD (copper metabolism MURR1 domain)-containing proteins are a part of the CCC (COMMD-CCDC22 [coiled-coil domain containing 22]-CCDC93 [coiled-coil domain containing 93]) complex facilitating endosomal trafficking of cell surface receptors. Hepatic COMMD1 inactivation decreases CCDC22 and CCDC93 protein levels, impairs the recycling of the LDLR (low-density lipoprotein receptor), and increases plasma low-density lipoprotein cholesterol levels in mice. However, whether any of the oth... Abstract
Cited 40 times since 2017 (5.9 per year) source: EuropePMC
The EMBO journal, Volume 36, Issue 18, 4 1 2017, Pages 2726-2741 Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication. Brustel J, Kirstein N, Izard F, Grimaud C, Prorok P, Cayrou C, Schotta G, Abdelsamie AF, Déjardin J, Méchali M, Baldacci G, Sardet C, Cadoret JC, Schepers A, Julien E
Among other targets, the protein lysine methyltransferase PR-Set7 induces histone H4 lysine 20 monomethylation (H4K20me1), which is the substrate for further methylation by the Suv4-20h methyltransferase. Although these enzymes have been implicated in control of replication origins, the specific contribution of H4K20 methylation to DNA replication remains unclear. Here, we show that H4K20 mutation in mammalian cells, unlike in Drosophila, partially impairs S-phase progression and protects from D... Abstract
Cited 8 times since 2017 (1.2 per year) source: EuropePMC
The Journal of general virology, Volume 98, Issue 8, 28 4 2017, Pages 2128-2142 Antibodies against the mono-methylated arginine-glycine repeat (MMA-RG) of the Epstein-Barr virus nuclear antigen 2 (EBNA2) identify potential cellular proteins targeted in viral transformation. Ayoubian H, Fröhlich T, Pogodski D, Flatley A, Kremmer E, Schepers A, Feederle R, Arnold GJ, Grässer FA
The Epstein-Barr virus is a human herpes virus with oncogenic potential. The virus-encoded nuclear antigen 2 (EBNA2) is a key mediator of viral tumorigenesis. EBNA2 features an arginine-glycine (RG) repeat at amino acids (aa)339-354 that is essential for the transformation of lymphocytes and contains symmetrically (SDMA) and asymmetrically (ADMA) di-methylated arginine residues. The SDMA-modified EBNA2 binds the survival motor neuron protein (SMN), thus mimicking SMD3, a cellular SDMA-containing... Abstract
Cited 12 times since 2017 (1.7 per year) source: EuropePMC
Oncotarget, Volume 8, Issue 17, 1 1 2017, Pages 28650-28659 Expression of HIF-1α in medullary thyroid cancer identifies a subgroup with poor prognosis. Lodewijk L, van Diest P, van der Groep P, Ter Hoeve N, Schepers A, Morreau J, Bonenkamp J, van Engen-van Grunsven A, Kruijff S, van Hemel B, Links T, Nieveen van Dijkum E, van Eeden S, Valk G, Borel Rinkes I, Vriens M
Background: Medullary thyroid cancer (MTC) comprises only 4% of all thyroid cancers and originates from the parafollicular C-cells. HIF-1α expression has been implied as an indicator of worse prognosis in various solid tumors. However, whether expression of HIF-1α is a prognosticator in MTC remained unclear. Our aim was to evaluate the prognostic value of HIF-1α in patients with MTC. Methods: All patients with MTC who were operated on between 1988 and 2014 in five tertiary referral centers in Th... Abstract
Cited 19 times since 2017 (2.6 per year) source: EuropePMC
RNA biology, Volume 14, Issue 9, 23 4 2017, Pages 1089-1098 Antibodies specific for nucleic acid modifications. Feederle R, Schepers A
Nucleotide modifications constitute marks in RNA and DNA that contribute to gene regulation, development and other cellular processes. The understanding of their intricate molecular roles has been hampered by the high number of different modifications, the lack of effective methods and tools for their detection and quantification as well as by their complex structure-function relationship. The recent development of RNA and DNA immunoprecipitation followed by high-throughput sequencing (RIP- and... Abstract
Cited 145 times since 2016 (19.4 per year) source: EuropePMC
Cancer cell, Volume 30, Issue 6, 1 1 2016, Pages 849-862 Characterization of Rare, Dormant, and Therapy-Resistant Cells in Acute Lymphoblastic Leukemia. Ebinger S, Özdemir EZ, Ziegenhain C, Tiedt S, Castro Alves C, Grunert M, Dworzak M, Lutz C, Turati VA, Enver T, Horny HP, Sotlar K, Parekh S, Spiekermann K, Hiddemann W, Schepers A, Polzer B, Kirsch S, Hoffmann M, Knapp B, Hasenauer J, Pfeifer H, Panzer-Grümayer R, Enard W, Gires O, Jeremias I
Tumor relapse is associated with dismal prognosis, but responsible biological principles remain incompletely understood. To isolate and characterize relapse-inducing cells, we used genetic engineering and proliferation-sensitive dyes in patient-derived xenografts of acute lymphoblastic leukemia (ALL). We identified a rare subpopulation that resembled relapse-inducing cells with combined properties of long-term dormancy, treatment resistance, and stemness. Single-cell and bulk expression profilin... Abstract
Cited 67 times since 2016 (8.5 per year) source: EuropePMC
Lab on a chip, Volume 16, Issue 14, 1 1 2016, Pages 2644-2653 Engineering a perfusable 3D human liver platform from iPS cells. Schepers A, Li C, Chhabra A, Seney BT, Bhatia S
In vitro models of human tissue are crucial to our ability to study human disease as well as develop safe and effective drug therapies. Models of single organs in static and microfluidic culture have been established and shown utility for modeling some aspects of health and disease; however, these systems lack multi-organ interactions that are critical to some aspects of drug metabolism and toxicity. Thus, as part of a consortium of researchers, we have developed a liver chip that meets the foll... Abstract
Cited 1 times since 2016 (0.1 per year) source: EuropePMC
Applied neuropsychology. Child, Volume 6, Issue 2, 6 1 2016, Pages 145-157 Neuropsychological presentation and adaptive skills in high-functioning adolescents with visual impairment: A preliminary investigation. Greenaway R, Pring L, Schepers A, Isaacs DP, Dale NJ
Studies in infants and young children with congenital visual impairment (VI) have indicated early developmental vulnerabilities, conversely research with older children and adults have highlighted areas of cognitive strength. A minimal amount is known, however, about the possible combination of strengths and weaknesses in adolescence, and this present study therefore aims to explore the neuropsychological presentation and adaptive behavior profile in high-functioning adolescents with congenital... Abstract
Cited 8 times since 2016 (1 per year) source: EuropePMC
Langenbeck's archives of surgery, Volume 401, Issue 3, 24 4 2016, Pages 365-373 Characteristics of contralateral carcinomas in patients with differentiated thyroid cancer larger than 1 cm. Lodewijk L, Kluijfhout WP, Kist JW, Stegeman I, Plukker JT, Nieveen van Dijkum EJ, Bonjer HJ, Bouvy ND, Schepers A, de Wilt JH, Netea-Maier RT, van der Hage JA, Burger JW, Ho G, Lee WS, Shen WT, Aronova A, Zarnegar R, Benay C, Mitmaker EJ, Sywak MS, Aniss AM, Kruijff S, James B, Grogan RH, Brunaud L, Hoch G, Pandolfi C, Ruan DT, Jones MD, Guerrero MA, Valk GD, Borel Rinkes IH, Vriens MR
Purpose: Traditionally, total thyroidectomy has been advocated for patients with tumors larger than 1 cm. However, according to the ATA and NCCN guidelines (2015, USA), patients with tumors up to 4 cm are now eligible for lobectomy. A rationale for adhering to total thyroidectomy might be the presence of contralateral carcinomas. The purpose of this study was to describe the characteristics of contralateral carcinomas in patients with differentiated thyroid cancer (DTC) larger than 1 cm. Methods... Abstract