Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
251 results
Cited 6 times since 2018 (1 per year) source: EuropePMC
American journal of physiology. Heart and circulatory physiology, Volume 315, Issue 3, 4 1 2018, Pages H669-H680 Acute stimulation of the soluble guanylate cyclase does not impact on left ventricular capacitance in normal and hypertrophied porcine hearts in vivo. Alogna A, Schwarzl M, Manninger M, Hamdani N, Zirngast B, Kloth B, Steendijk P, Verderber J, Zweiker D, Westermann D, Blankenberg S, Maechler H, Tschöpe C, Linke WA, Marsche G, Pieske BM, Post H
Experimental data indicate that stimulation of the nitric oxide-soluble guanylate cyclase(sGC)-cGMP-PKG pathway can increase left ventricular (LV) capacitance via phosphorylation of the myofilamental protein titin. We aimed to test whether acute pharmacological sGC stimulation with BAY 41-8543 would increase LV capacitance via titin phosphorylation in healthy and deoxycorticosteroneacetate (DOCA)-induced hypertensive pigs. Nine healthy Landrace pigs and 7 pigs with DOCA-induced hypertension and... Abstract
Cited 6 times since 2018 (1 per year) source: EuropePMC
International journal of cardiology, Volume 268, 8 2 2018, Pages 156-161 Right heart function interacts with left ventricular remodeling after CRT: A pressure volume loop study. Schmeisser A, Rauwolf T, Ghanem A, Groscheck T, Adolf D, Grothues F, Fischbach K, Kosiek O, Huth C, Kropf S, Lange S, Luani B, Smid J, Schäfer MH, Schreiber J, Tanev I, Wengler F, Yeritsyan NB, Steendijk P, Braun-Dullaeus RC
Background: Right ventricular (RV) dysfunction is recognized as a cardinal prognostic marker in systolic heart failure patients. Conflicting data exist on the interaction of RV function and left ventricular (LV) reverse remodeling after cardiac resynchronization therapy (CRT). This prospective monocentric trial was set up to assess the predictive value of baseline RV function and corresponding RV-pulmonary artery (PA) coupling on LV reverse remodeling after CRT. Methods: 110 patients with a CRT... Abstract
Cited 11 times since 2017 (1.7 per year) source: EuropePMC
PloS one, Volume 12, Issue 12, 5 1 2017, Pages e0189139 Z-disc protein CHAPb induces cardiomyopathy and contractile dysfunction in the postnatal heart. van Eldik W, den Adel B, Monshouwer-Kloots J, Salvatori D, Maas S, van der Made I, Creemers EE, Frank D, Frey N, Boontje N, van der Velden J, Steendijk P, Mummery C, Passier R, Beqqali A
Aims: The Z-disc is a crucial structure of the sarcomere and is implicated in mechanosensation/transduction. Dysregulation of Z-disc proteins often result in cardiomyopathy. We have previously shown that the Z-disc protein Cytoskeletal Heart-enriched Actin-associated Protein (CHAP) is essential for cardiac and skeletal muscle development. Furthermore, the CHAP gene has been associated with atrial fibrillation in humans. Here, we studied the misregulated expression of CHAP isoforms in heart disea... Abstract
Cited 10 times since 2016 (1.3 per year) source: EuropePMC
Circulation, Volume 134, Issue 16, 14 2 2016, Pages 1163-1175 Thoracic Epidural Anesthesia Reduces Right Ventricular Systolic Function With Maintained Ventricular-Pulmonary Coupling. Wink J, de Wilde RB, Wouters PF, van Dorp EL, Veering BT, Versteegh MI, Aarts LP, Steendijk P
Background: Blockade of cardiac sympathetic fibers by thoracic epidural anesthesia may affect right ventricular function and interfere with the coupling between right ventricular function and right ventricular afterload. Our main objectives were to study the effects of thoracic epidural anesthesia on right ventricular function and ventricular-pulmonary coupling. Methods: In 10 patients scheduled for lung resection, right ventricular function and its response to increased afterload, induced by te... Abstract
Cited 8 times since 2016 (1 per year) source: EuropePMC
American journal of physiology. Heart and circulatory physiology, Volume 311, Issue 4, 9 2 2016, Pages H1014-H1023 Hyperaldosteronism induces left atrial systolic and diastolic dysfunction. Reil JC, Tauchnitz M, Tian Q, Hohl M, Linz D, Oberhofer M, Kaestner L, Reil GH, Thiele H, Steendijk P, Böhm M, Neuberger HR, Lipp P
Patients with hypertension and hyperaldosteronism show an increased risk of stroke compared with patients with essential hypertension. Aim of the study was to assess the effects of aldosterone on left atrial function in rats as a potential contributor to thromboembolism. Osmotic mini-pumps delivering 1.5 μg aldosterone/h were implanted in rats subcutaneously (Aldo, n = 39; controls, n = 38). After 8 wk, left ventricular pressure-volume analysis of isolated working hearts was performed, and left... Abstract
Cited 16 times since 2016 (2 per year) source: EuropePMC
Critical care medicine, Volume 44, Issue 3, 1 1 2016, Pages e158-67 Inotropic Effects of Experimental Hyperthermia and Hypothermia on Left Ventricular Function in Pigs-Comparison With Dobutamine. Alogna A, Manninger M, Schwarzl M, Zirngast B, Steendijk P, Verderber J, Zweiker D, Maechler H, Pieske BM, Post H
Objectives: The results from the recent Targeted Temperature Management trial raised the question whether cooling or merely the avoidance of fever mediates better neurologic outcome in resuscitated patients. As temperature per se is a major determinant of cardiac function, we characterized the effects of hyperthermia (40.5°C), normothermia (38.0°C), and mild hypothermia (33.0°C) on left ventricular contractile function in healthy pigs and compared them with dobutamine infusion. Design: Animal st... Abstract
Cited 8 times since 2015 (0.9 per year) source: EuropePMC
Journal of cardiovascular translational research, Volume 8, Issue 7, 17 3 2015, Pages 393-403 Autologous Mesenchymal Stem Cells Show More Benefit on Systolic Function Compared to Bone Marrow Mononuclear Cells in a Porcine Model of Chronic Myocardial Infarction. van der Spoel TI, Gathier WA, Koudstaal S, van Slochteren F, Of Lorkeers SJ, Sluijter JP, Hoefer IE, Steendijk P, Cramer MJ, Doevendans PA, van Belle E, Chamuleau SA
Cardiac cell therapy is a strategy to treat patients with chronic myocardial infarction (MI). No consensus exists regarding the optimal cell type. First, a comparison between autologous bone marrow-derived mononuclear cells (BMMNC) and mesenchymal stem cells (MSC) on therapeutic efficacy after MI was performed. Next, the effect of repetitive, NOGA-guided transendocardial injection was determined via a crossover design. Nineteen pigs were allocated in three groups: (1) placebo (at 4 and 8 weeks),... Abstract
Cited 55 times since 2015 (6.3 per year) source: EuropePMC
American journal of physiology. Heart and circulatory physiology, Volume 309, Issue 9, 4 1 2015, Pages H1407-18 A porcine model of hypertensive cardiomyopathy: implications for heart failure with preserved ejection fraction. Schwarzl M, Hamdani N, Seiler S, Alogna A, Manninger M, Reilly S, Zirngast B, Kirsch A, Steendijk P, Verderber J, Zweiker D, Eller P, Höfler G, Schauer S, Eller K, Maechler H, Pieske BM, Linke WA, Casadei B, Post H
Heart failure with preserved ejection fraction (HFPEF) evolves with the accumulation of risk factors. Relevant animal models to identify potential therapeutic targets and to test novel therapies for HFPEF are missing. We induced hypertension and hyperlipidemia in landrace pigs (n = 8) by deoxycorticosteroneacetate (DOCA, 100 mg/kg, 90-day-release subcutaneous depot) and a Western diet (WD) containing high amounts of salt, fat, cholesterol, and sugar for 12 wk. Compared with weight-matched contro... Abstract
Cited 2 times since 2015 (0.2 per year) source: EuropePMC
Cardiorenal medicine, Volume 5, Issue 1, 16 3 2015, Pages 69-78 Neuronal nitric oxide synthase-dependent amelioration of diastolic dysfunction in rats with chronic renocardiac syndrome. Bongartz LG, Soni S, Cramer MJ, Steendijk P, Gaillard CA, Verhaar MC, Doevendans PA, van Veen TA, Joles JA, Braam B
We have recently described the chronic renocardiac syndrome (CRCS) in rats with renal failure, cardiac dysfunction and low nitric oxide (NO) availability by combining subtotal nephrectomy and transient low-dose NO synthase (NOS) inhibition. Cardiac gene expression of the neuronal isoform of NOS (nNOS) was induced. Hence, we studied the role of nNOS, in vivo cardiac function and β-adrenergic response in our CRCS model by micromanometer/conductance catheter. Left ventricular (LV) hemodynamics were... Abstract
Cited 11 times since 2015 (1.2 per year) source: EuropePMC
Acta physiologica (Oxford, England), Volume 213, Issue 3, 9 2 2015, Pages 653-663 Mild hypothermia induces incomplete left ventricular relaxation despite spontaneous bradycardia in pigs. Schwarzl M, Alogna A, Zirngast B, Steendijk P, Verderber J, Zweiker D, Huber S, Maechler H, Pieske BM, Post H
Aim: Mild hypothermia (MH) decreases left ventricular (LV) end-diastolic capacitance. We sought to clarify whether this results from incomplete relaxation. Methods: Ten anaesthetized pigs were cooled from normothermia (NT, 38 °C) to MH (33 °C). LV end-diastolic pressure (LVPed), volume (LVVed) and pressure-volume relationships (EDPVRs) were determined during stepwise right atrial pacing. LV capacitance (i.e. LVVed at LVPed of 10 mmHg, LV VPed10) was derived from the EDPVR. Pacing-induced changes... Abstract
Cited 24 times since 2014 (2.5 per year) source: EuropePMC
Journal of molecular and cellular cardiology, Volume 79, 5 1 2014, Pages 244-253 Clinical symptoms of right ventricular failure in experimental chronic pressure load are associated with progressive diastolic dysfunction. Borgdorff MA, Koop AM, Bloks VW, Dickinson MG, Steendijk P, Sillje HH, van Wiechen MP, Berger RM, Bartelds B
Background: Right ventricular failure (RVF) due to pressure load is a major cause of death in congenital heart diseases and pulmonary hypertension. The mechanisms of RVF are unknown. We used an experimental approach based upon clinical signs of RVF to delineate functional and biological processes associated with RVF. Methods and results: Wistar rats were subjected to a pulmonary artery banding (PAB n=12) or sham surgery (CON, n=7). After 52±5days, 5/12 PAB rats developed clinical symptoms of RVF... Abstract
Cited 4 times since 2014 (0.4 per year) source: EuropePMC
International journal of cardiology, Volume 183, 3 1 2014, Pages 249-257 Abnormal sinoatrial node development resulting from disturbed vascular endothelial growth factor signaling. Calkoen EE, Vicente-Steijn R, Hahurij ND, van Munsteren CJ, Roest AA, DeRuiter MC, Steendijk P, Schalij MJ, Gittenberger-de Groot AC, Blom NA, Jongbloed MR
Background: Sinus node dysfunction is frequently observed in patients with congenital heart disease (CHD). Variants in the Vascular Endothelial Growth Factor-A (VEGF) pathway are associated with CHD. In Vegf(120/120) mice, over-expressing VEGF120, a reduced sinoatrial node (SAN) volume was suggested. Aim of the study is to assess the effect of VEGF over-expression on SAN development and function. Methods: Heart rate was measured in Vegf(120/120) and wildtype (WT) embryos during high frequency ul... Abstract
Cited 14 times since 2014 (1.4 per year) source: EuropePMC
International journal of cardiology, Volume 176, Issue 3, 3 1 2014, Pages 788-793 RP105 deficiency aggravates cardiac dysfunction after myocardial infarction in mice. Louwe MC, Karper JC, de Vries MR, Nossent AY, Bastiaansen AJ, van der Hoorn JW, Willems van Dijk K, Rensen PC, Steendijk P, Smit JW, Quax PH
Background: Toll-like receptor-4 (TLR4), a receptor of the innate immune system, is suggested to have detrimental effects on cardiac function after myocardial infarction (MI). RP105 (CD180) is a TLR4 homolog lacking the intracellular signaling domain that competitively inhibits TLR4-signaling. Thus, we hypothesized that RP105 deficiency, by amplifying TLR4 signaling, would lead to aggravated cardiac dysfunction after MI. Methods and results: First, whole blood from RP105-/- and wild-type (WT) ma... Abstract
Cited 20 times since 2014 (2 per year) source: EuropePMC
Journal of cardiovascular electrophysiology, Volume 25, Issue 9, 24 4 2014, Pages 1012-1020 Impact of pacing site on QRS duration and its relationship to hemodynamic response in cardiac resynchronization therapy for congestive heart failure. Derval N, Bordachar P, Lim HS, Sacher F, Ploux S, Laborderie J, Steendijk P, Deplagne A, Ritter P, Garrigue S, Denis A, Hocini M, Haissaguerre M, Clementy J, Jaïs P
Introduction: Recent studies have demonstrated that left ventricular (LV) pacing site is a critical parameter in optimizing cardiac resynchronization therapy (CRT). The present study evaluates the effect of pacing from different LV locations on QRS duration (QRSd) and their relationship to acute hemodynamic response in congestive heart failure patients. Methods and results: Thirty-five patients with nonischemic dilated cardiomyopathy and left bundle branch block referred for CRT device implantat... Abstract
Cited 27 times since 2014 (2.7 per year) source: EuropePMC
American journal of physiology. Heart and circulatory physiology, Volume 307, Issue 3, 30 5 2014, Pages H361-9 Sildenafil treatment in established right ventricular dysfunction improves diastolic function and attenuates interstitial fibrosis independent from afterload. Borgdorff MA, Bartelds B, Dickinson MG, van Wiechen MP, Steendijk P, de Vroomen M, Berger RM
Right ventricular (RV) function is an important determinant of prognosis in congenital heart diseases, pulmonary hypertension, and heart failure. Preventive sildenafil treatment has been shown to enhance systolic RV function and improve exercise capacity in a model of fixed RV pressure load. However, it is unknown whether sildenafil has beneficial effects when treatment is started in established RV dysfunction, which is clinically more relevant. Our aim was to assess the effects of sildenafil tr... Abstract
Cited 5 times since 2014 (0.5 per year) source: EuropePMC
TheScientificWorldJournal, Volume 2014, 23 4 2014, Pages 531324 Echocardiographic assessment of embryonic and fetal mouse heart development: a focus on haemodynamics and morphology. Hahurij ND, Calkoen EE, Jongbloed MR, Roest AA, Gittenberger-de Groot AC, Poelmann RE, De Ruiter MC, van Munsteren CJ, Steendijk P, Blom NA
Background: Heart development is a complex process, and abnormal development may result in congenital heart disease (CHD). Currently, studies on animal models mainly focus on cardiac morphology and the availability of hemodynamic data, especially of the right heart half, is limited. Here we aimed to assess the morphological and hemodynamic parameters of normal developing mouse embryos/fetuses by using a high-frequency ultrasound system. Methods: A timed breeding program was initiated with a WT m... Abstract
Cited 21 times since 2013 (2 per year) source: EuropePMC
Critical care medicine, Volume 41, Issue 12, 1 1 2013, Pages e401-10 Mild hypothermia attenuates circulatory and pulmonary dysfunction during experimental endotoxemia. Schwarzl M, Seiler S, Wallner M, von Lewinski D, Huber S, Maechler H, Steendijk P, Zelzer S, Truschnig-Wilders M, Obermayer-Pietsch B, Lueger A, Pieske BM, Post H
Objective: We tested whether mild hypothermia impacts on circulatory and respiratory dysfunction during experimental endotoxemia. Design: Randomized controlled prospective experimental study. Setting: Large animal facility, Medical University of Graz, Austria. Subjects: Thirteen anesthetized and mechanically ventilated pigs. Interventions: Lipopolysaccharide was administered for 4 hours. With the beginning of lipopolysaccharide infusion, animals were assigned to either normothermia (38°C, n = 7)... Abstract
Cited 35 times since 2013 (3.3 per year) source: EuropePMC
International journal of cardiology, Volume 169, Issue 3, 7 1 2013, Pages 183-189 A cornerstone of heart failure treatment is not effective in experimental right ventricular failure. Borgdorff MA, Bartelds B, Dickinson MG, Steendijk P, Berger RM
Background: Right ventricular (RV) failure due to increased pressure load causes significant morbidity and mortality in patients with congenital heart diseases and pulmonary arterial hypertension. It is unknown whether renin-angiotensin-aldosterone-system (RAAS) inhibition (the cornerstone of left ventricular failure treatment) is effective in RV failure. We investigated the effects of combination treatment of aldosterone-blocker eplerenone+angiotensin II receptor blocker losartan (Ep/Lo) on RV... Abstract
Cited 15 times since 2013 (1.4 per year) source: EuropePMC
Anesthesiology, Volume 118, Issue 6, 1 1 2013, Pages 1385-1394 Xenon and isoflurane reduce left ventricular remodeling after myocardial infarction in the rat. Roehl AB, Funcke S, Becker MM, Goetzenich A, Bleilevens C, Rossaint R, Steendijk P, Hein M
Background: Xenon and isoflurane are known to have cardioprotective properties. We tested the hypothesis that these anesthetics positively influence myocardial remodeling 28 days after experimental perioperative myocardial infarction and compared their effects. Methods: A total of 60 male Sprague-Dawley rats were subjected to 60 min of coronary artery occlusion and 120 min of reperfusion. Prior to ischemia, the animals were randomized for the different narcotic regimes (0.6 vol% isoflurane, 70 v... Abstract
Cited 35 times since 2013 (3.2 per year) source: EuropePMC
American journal of physiology. Heart and circulatory physiology, Volume 305, Issue 3, 31 5 2013, Pages H354-64 Distinct loading conditions reveal various patterns of right ventricular adaptation. Borgdorff MA, Bartelds B, Dickinson MG, Steendijk P, de Vroomen M, Berger RM
Right ventricular (RV) failure due to chronically abnormal loading is a main determinant of outcome in pulmonary hypertension (PH) and congenital heart disease. However, distinct types of RV loading have been associated with different outcomes. To determine whether the adaptive RV response depends on loading type, we compared hemodynamics, exercise, and hypertrophy in models of pressure overload due to pulmonary artery banding (PAB), pressure overload due to PH, combined pressure and volume over... Abstract