Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
140 results
Cited 43 times since 2015 (4.8 per year) source: EuropePMC
Nature communications, Volume 6, 8 2 2015, Pages 7030 Expression of the vault RNA protects cells from undergoing apoptosis. Amort M, Nachbauer B, Tuzlak S, Kieser A, Schepers A, Villunger A, Polacek N
Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein-Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA u... Abstract
Cited 38 times since 2015 (4.2 per year) source: EuropePMC
Surgery, Volume 158, Issue 5, 6 1 2015, Pages 1323-1330 Intraoperative guidance in parathyroid surgery using near-infrared fluorescence imaging and low-dose Methylene Blue. Tummers QR, Schepers A, Hamming JF, Kievit J, Frangioni JV, van de Velde CJ, Vahrmeijer AL
Background: Identification of diseased and normal parathyroid glands during parathyroid surgery can be challenging. The aim of this study was to assess whether near-infrared (NIR) fluorescence imaging using administration of a low-dose Methylene Blue (MB) at the start of the operation could provide optical guidance during parathyroid surgery and assist in the detection of parathyroid adenomas. Methods: Patients diagnosed with primary hyperparathyroidism planned for parathyroidectomy were include... Abstract
Cited 3 times since 2014 (0.3 per year) source: EuropePMC
Behavior modification, Volume 39, Issue 3, 16 3 2014, Pages 390-412 Cognitive behavior therapy for anxiety in people with dementia: a clinician guideline for a person-centered approach. Charlesworth G, Sadek S, Schepers A, Spector A
This article describes a 10-session cognitive-behavioral therapy (CBT) used in a randomized controlled trial with people with anxiety and mild-to-moderate dementia. The aim of the therapy is to reduce symptoms of anxiety by increasing a sense of safety and self-efficacy. The therapy is characterized by a person-centered approach to CBT, using individual tailoring to accommodate for cognitive deficits and other challenges. Three phases of therapy are described: (a) socialization to model (includi... Abstract
Cited 31 times since 2013 (2.8 per year) source: EuropePMC
Cyberpsychology, behavior and social networking, Volume 16, Issue 7, 25 4 2013, Pages 497-503 Display of alcohol use on Facebook: a content analysis. Beullens K, Schepers A
The present study has two main objectives. First, to examine how alcohol use is portrayed in the visual and textual references on Facebook, and second, to determine how friends react to these alcohol-related postings. A theoretically based content analysis of 160 Facebook profiles generated 2,575 pictures and 92 status updates referring to alcohol use, which represented about 6.50% of the pictures in the total sample and 2.90% of the status updates. These visual and textual references, as well a... Abstract
Cited 56 times since 2012 (4.9 per year) source: EuropePMC
Nature structural & molecular biology, Volume 20, Issue 1, 2 1 2012, Pages 73-81 Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay. Hoefig KP, Rath N, Heinz GA, Wolf C, Dameris J, Schepers A, Kremmer E, Ansel KM, Heissmeyer V
The exoRNase Eri1 inhibits RNA interference and trims the 5.8S rRNA 3' end. It also binds to the stem-loop of histone mRNAs, but the functional importance of this interaction remains elusive. Histone mRNAs are normally degraded at the end of S phase or after pharmacological inhibition of replication. Both processes are impaired in Eri1-deficient mouse cells, which instead accumulate oligouridylated histone mRNAs. Eri1 trims the mature histone mRNAs by two unpaired nucleotides at the 3'... Abstract
Cited 30 times since 2012 (2.6 per year) source: EuropePMC
Cardiovascular research, Volume 97, Issue 2, 14 2 2012, Pages 311-320 Complement factor C5a as mast cell activator mediates vascular remodelling in vein graft disease. de Vries MR, Wezel A, Schepers A, van Santbrink PJ, Woodruff TM, Niessen HW, Hamming JF, Kuiper J, Bot I, Quax PH
Aims: Failure of vein graft conduits due to vein graft thickening, accelerated atherosclerosis, and subsequent plaque rupture is applicable to 50% of all vein grafts within 10 years. New potential therapeutic targets to treat vein graft disease may be found in components of the innate immune system, such as mast cells and complement factors, which are known to be involved in atherosclerosis and plaque destabilization. Interestingly, mast cells can be activated by complement factor C5a and, there... Abstract
Cited 3 times since 2012 (0.3 per year) source: EuropePMC
Protein expression and purification, Volume 86, Issue 1, 26 4 2012, Pages 7-11 Efficient expression and purification of tag-free Epstein-Barr virus EBNA1 protein in Escherichia coli by auto-induction. Mayer CE, Geerlof A, Schepers A
Epstein-Barr nuclear antigen 1 (EBNA1) is the essential Epstein-Barr virus (EBV) protein at the interface between the EBV genome and the host chromatin. It is EBNA1's task to guarantee replication and segregation of the multicopy closed circular viral genome in infected cells. While EBNA1's functions are relatively well understood, little is known about the molecular mechanisms of EBNA1 mediating chromatin tethering and DNA replication. To characterize those, purified EBNA1 would be a... Abstract
Cited 11 times since 2012 (0.9 per year) source: EuropePMC
The Journal of cell biology, Volume 198, Issue 4, 13 2 2012, Pages 509-528 Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus. Papior P, Arteaga-Salas JM, Günther T, Grundhoff A, Schepers A
Whether or not metazoan replication initiates at random or specific but flexible sites is an unsolved question. The lack of sequence specificity in origin recognition complex (ORC) DNA binding complicates genome-scale chromatin immunoprecipitation (ChIP)-based studies. Epstein-Barr virus (EBV) persists as chromatinized minichromosomes that are replicated by the host replication machinery. We used EBV to investigate the link between zones of pre-replication complex (pre-RC) assembly, replication... Abstract
Cited 29 times since 2012 (2.5 per year) source: EuropePMC
Frontiers in oncology, Volume 2, 25 4 2012, Pages 77 The splicing mutant of the human tumor suppressor protein DFNA5 induces programmed cell death when expressed in the yeast Saccharomyces cerevisiae. Van Rossom S, Op de Beeck K, Franssens V, Swinnen E, Schepers A, Ghillebert R, Caldara M, Van Camp G, Winderickx J
DFNA5 was first identified as a gene responsible for autosomal dominant deafness. Different mutations were found, but they all resulted in exon 8 skipping during splicing and premature termination of the protein. Later, it became clear that the protein also has a tumor suppression function and that it can induce apoptosis. Epigenetic silencing of the DFNA5 gene is associated with different types of cancers, including gastric and colorectal cancers as well as breast tumors. We introduced the wild... Abstract
Cited 82 times since 2012 (6.8 per year) source: EuropePMC
Cold Spring Harbor perspectives in biology, Volume 4, Issue 4, 1 1 2012, Pages a007989 Wnt signaling, stem cells, and cancer of the gastrointestinal tract. Schepers A, Clevers H
The Wnt signaling pathway was originally uncovered as one of the prototype developmental signaling cascades in invertebrates as well as in vertebrates. The first indication that Wnt signaling also plays a role in the adult animal came from the study of the intestine of Tcf-4 (Tcf7L2) knockout mice. The gastrointestinal epithelium continuously self-renews over the lifetime of an organism and is, in fact, the most rapidly self-renewing tissue of the mammalian body. Recent studies indicate that Wnt... Abstract
Cited 17 times since 2011 (1.4 per year) source: EuropePMC
PloS one, Volume 6, Issue 12, 6 1 2011, Pages e28638 Upregulation of the cell-cycle regulator RGC-32 in Epstein-Barr virus-immortalized cells. Schlick SN, Wood CD, Gunnell A, Webb HM, Khasnis S, Schepers A, West MJ
Epstein-Barr virus (EBV) is implicated in the pathogenesis of multiple human tumours of lymphoid and epithelial origin. The virus infects and immortalizes B cells establishing a persistent latent infection characterized by varying patterns of EBV latent gene expression (latency 0, I, II and III). The CDK1 activator, Response Gene to Complement-32 (RGC-32, C13ORF15), is overexpressed in colon, breast and ovarian cancer tissues and we have detected selective high-level RGC-32 protein expression in... Abstract
Cited 178 times since 2011 (14.2 per year) source: EuropePMC
Science (New York, N.Y.), Volume 334, Issue 6056, 1 1 2011, Pages 686-690 Drosophila CENH3 is sufficient for centromere formation. Mendiburo MJ, Padeken J, Fülöp S, Schepers A, Heun P
CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecule... Abstract
Cited 9 times since 2011 (0.7 per year) source: EuropePMC
Atherosclerosis, Volume 220, Issue 1, 25 4 2011, Pages 86-92 C1-esterase inhibitor protects against early vein graft remodeling under arterial blood pressure. Krijnen PA, Kupreishvili K, de Vries MR, Schepers A, Stooker W, Vonk AB, Eijsman L, Van Hinsbergh VW, Zeerleder S, Wouters D, van Ham M, Quax PH, Niessen HW
Objectives: Arterial pressure induced vein graft injury can result in endothelial loss, accelerated atherosclerosis and vein graft failure. Inflammation, including complement activation, is assumed to play a pivotal role herein. Here, we analyzed the effects of C1-esterase inhibitor (C1inh) on early vein graft remodeling. Methods: Human saphenous vein graft segments (n=8) were perfused in vitro with autologous blood either supplemented or not with purified human C1inh at arterial pressure for 6h... Abstract
Cited 46 times since 2011 (3.6 per year) source: EuropePMC
Ageing research reviews, Volume 11, Issue 1, 29 5 2011, Pages 67-77 A systematic review of 'knowledge of dementia' outcome measures. Spector A, Orrell M, Schepers A, Shanahan N
Knowledge of dementia measures are key to identifying areas of misinformation and establishing knowledge levels, thus guiding educational programmes and interventions. A three-step literature search was undertaken to identify measures of knowledge in dementia. An evaluation framework was employed articulating quality indicators for the psychometric properties of measures, based on their development and use within research studies. Five measures were identified: the Alzheimer's Disease Knowl... Abstract
Cited 2 times since 2011 (0.2 per year) source: EuropePMC
The Journal of arthroplasty, Volume 27, Issue 3, 31 5 2011, Pages 454-460 A prospective randomized clinical trial comparing tibial baseplate fixation with or without screws in total knee arthroplasty: a radiographic evaluation. Schepers A, Cullingworth L, van der Jagt DR
In a randomized prospective study, we compared the use of tibial screws with screwless tibial baseplate fixation in uncemented total knee arthroplasty and report the results of 154 prostheses at a mean follow-up of 5 years. Patients were mobilized within 24 hours and continued with partial weight-bearing for up to 6 weeks postoperatively. There were no significant differences in radiologic results and revision rates. Radiographic outcomes do not seem to be influenced by the use of screws in tibi... Abstract
Cited 9 times since 2011 (0.7 per year) source: EuropePMC
PloS one, Volume 6, Issue 5, 16 3 2011, Pages e18609 The dyad symmetry element of Epstein-Barr virus is a dominant but dispensable replication origin. Ott E, Norio P, Ritzi M, Schildkraut C, Schepers A
OriP, the latent origin of Epstein-Barr virus (EBV), consists of two essential elements: the dyad symmetry (DS) and the family of repeats (FR). The function of these elements has been predominantly analyzed in plasmids transfected into transformed cells. Here, we examined the molecular functions of DS in its native genomic context and at an ectopic position in the mini-EBV episome. Mini-EBV plasmids contain 41% of the EBV genome including all information required for the proliferation of human B... Abstract
Cited 17 times since 2011 (1.3 per year) source: EuropePMC
Cellular and molecular life sciences : CMLS, Volume 68, Issue 22, 2 1 2011, Pages 3741-3756 Different roles of the human Orc6 protein in the replication initiation process. Thomae AW, Baltin J, Pich D, Deutsch MJ, Ravasz M, Zeller K, Gossen M, Hammerschmidt W, Schepers A
In eukaryotes, binding of the six-subunit origin recognition complex (ORC) to DNA provides an interactive platform for the sequential assembly of pre-replicative complexes. This process licenses replication origins competent for the subsequent initiation step. Here, we analyze the contribution of human Orc6, the smallest subunit of ORC, to DNA binding and pre-replicative complex formation. We show that Orc6 not only interacts with Orc1-Orc5 but also with the initiation factor Cdc6. Biochemical a... Abstract
Cited 7 times since 2010 (0.5 per year) source: EuropePMC
The Journal of surgical research, Volume 164, Issue 1, 2 1 2010, Pages e91-7 Impact of complications after surgery for colorectal liver metastasis on patient survival. Schepers A, Mieog S, van de Burg BB, van Schaik J, Liefers GJ, Marang-van de Mheen PJ
Background: In some patients with colorectal liver metastases it is not clear whether liver resection or isolated liver perfusion is the best treatment option. For instance, the risk of complications after surgery may be so substantial and affect subsequent survival. Aim of the present study is to compare complication occurrence and its effect on survival after liver resection and perfusion. Methods: Patient records of all 225 patients with colorectal liver metastases treated with liver resectio... Abstract
Cited 18 times since 2010 (1.3 per year) source: EuropePMC
Clinical orthopaedics and related research, Volume 468, Issue 9, 8 2 2010, Pages 2485-2494 Developmental dysplasia of the hip: open reduction as a risk factor for substantial osteonecrosis. Firth GB, Robertson AJ, Schepers A, Fatti L
Background: Kalamchi and MacEwen (K&M) described a four-group scheme for classifying osteonecrosis (ON) following treatment for developmental dysplasia of the hip (DDH). However, the four groups can overlap in radiographic appearance, making assessment difficult. Questions/purposes: We (1) describe a simplified K&M classification; (2) determined whether the simplified classification was reliable; and (3) assessed whether differences in the type of reduction or age at reduction resulted i... Abstract
Cited 24 times since 2010 (1.7 per year) source: EuropePMC
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, Volume 18, Issue 1, 1 1 2010, Pages 63-77 Why are we where we are? Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches. Schepers A, Papior P
DNA replication initiates from origins of replication following a strict sequential activation programme and a conserved temporal order of activation. The number of replication initiation sites varies between species, according to the complexity of the genomes, with an average spacing of 100,000 bp. In contrast to yeast genomes, the location and definition of origins in mammalian genomes has been elusive. Historically, mammalian replication initiation sites have been mapped in situ by systematic... Abstract