Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
140 results
Cited 8 times since 2005 (0.4 per year) source: EuropePMC
Journal of the American College of Cardiology, Volume 46, Issue 6, 1 1 2005, Pages 1093-1100 Lipoprotein lipase gene polymorphisms and the risk of target vessel revascularization after percutaneous coronary intervention. Monraats PS, Rana JS, Nierman MC, Pires NM, Zwinderman AH, Kastelein JJ, Kuivenhoven JA, de Maat MP, Rittersma SZ, Schepers A, Doevendans PA, de Winter RJ, Tio RA, Frants RR, Quax PH, van der Laarse A, van der Wall EE, Jukema JW
Objectives: We sought to identify polymorphisms in genes that predispose to restenosis. Background: Variations in the lipoprotein lipase (LPL) gene have been implicated in a number of pathophysiologic conditions associated with coronary heart disease. The present study examines the impact of polymorphisms in the LPL gene on restenosis (defined by target vessel revascularization [TVR]) in a large patient population undergoing percutaneous coronary intervention (PCI). A mouse model for restenosis... Abstract
Cited 15 times since 2005 (0.8 per year) source: EuropePMC
Cardiovascular research, Volume 68, Issue 3, 15 3 2005, Pages 415-424 Histopathologic alterations following local delivery of dexamethasone to inhibit restenosis in murine arteries. Pires NM, Schepers A, van der Hoeven BL, de Vries MR, Boesten LS, Jukema JW, Quax PH
Objective: Dexamethasone-eluting stents are currently under evaluation to prevent post-angioplasty restenosis. The efficacy and safety of dexamethasone as an anti-restenotic agent is still unclear. We assess the effect of perivascular delivery of dexamethasone on vascular pathology in a mouse model of restenosis. Methods and results: In this study we investigate the ability of both systemic and local dexamethasone treatment to inhibit neointima formation after cuff placement around C57BL/6 mouse... Abstract
Cited 77 times since 2005 (4 per year) source: EuropePMC
The EMBO journal, Volume 24, Issue 7, 17 3 2005, Pages 1406-1417 Cell cycle regulation of chromatin at an origin of DNA replication. Zhou J, Chau CM, Deng Z, Shiekhattar R, Spindler MP, Schepers A, Lieberman PM
Selection and licensing of mammalian DNA replication origins may be regulated by epigenetic changes in chromatin structure. The Epstein-Barr virus (EBV) origin of plasmid replication (OriP) uses the cellular licensing machinery to regulate replication during latent infection of human cells. We found that the minimal replicator sequence of OriP, referred to as the dyad symmetry (DS), is flanked by nucleosomes. These nucleosomes were subject to cell cycle-dependent chromatin remodeling and histone... Abstract
Cited 20 times since 2004 (1 per year) source: EuropePMC
Inorganic chemistry, Volume 43, Issue 26, 1 1 2004, Pages 8461-8469 Rare-earth quinolinates: infrared-emitting molecular materials with a rich structural chemistry. Van Deun R, Fias P, Nockemann P, Schepers A, Parac-Vogt TN, Van Hecke K, Van Meervelt L, Binnemans K
Near-infrared-emitting rare-earth chelates based on 8-hydroxyquinoline have appeared frequently in recent literature, because they are promising candidates for active components in near-infrared-luminescent optical devices, such as optical amplifiers, organic light-emitting diodes, .... Unfortunately, the absence of a full structural investigation of these rare-earth quinolinates is hampering the further development of rare-earth quinolinate based materials, because the luminescence output canno... Abstract
Cited 8 times since 2004 (0.4 per year) source: EuropePMC
Gene, Volume 343, Issue 1, 1 1 2004, Pages 91-97 Efficient somatic gene targeting in the lymphoid human cell line DG75. Feederle R, Delecluse HJ, Rouault JP, Schepers A, Hammerschmidt W
Among the different approaches used to define the function of a protein of interest, alteration and/or deletion of its encoding gene is the most direct strategy. Homologous recombination between the chromosomal gene locus and an appropriately designed targeting vector results in an alteration or knockout of the gene of interest. Homologous recombination is easily performed in yeast or in murine embryonic stem cells, but is cumbersome in more differentiated and diploid somatic cell lines. Here we... Abstract
Cited 26 times since 2004 (1.3 per year) source: EuropePMC
European journal of radiology, Volume 52, Issue 3, 1 1 2004, Pages 240-245 The value of MR angiography techniques in the detection of head and neck paragangliomas. van den Berg R, Schepers A, de Bruïne FT, Liauw L, Mertens BJ, van der Mey AG, van Buchem MA
Objective: The objective of this study was to compare three-dimensional phase-contrast angiography (3D PCA), 2D time-of-flight (2D TOF), and 3D TOF magnetic resonance (MR) angiography and a proton density weighted technique in terms of their ability to detect head and neck paragangliomas. Materials and methods: 14 patients with 29 paragangliomas were examined at 1.5 T. Three MR angiography sequences (3D PCA, 2D TOF, and multi-slab 3D TOF) and a proton density (PD) weighted sequence were reviewed... Abstract
Cited 96 times since 2004 (4.8 per year) source: EuropePMC
The Journal of experimental medicine, Volume 199, Issue 10, 1 1 2004, Pages 1409-1420 CD8 T cell recognition of endogenously expressed epstein-barr virus nuclear antigen 1. Lee SP, Brooks JM, Al-Jarrah H, Thomas WA, Haigh TA, Taylor GS, Humme S, Schepers A, Hammerschmidt W, Yates JL, Rickinson AB, Blake NW
The Epstein-Barr virus (EBV) nuclear antigen (EBNA)1 contains a glycine-alanine repeat (GAr) domain that appears to protect the antigen from proteasomal breakdown and, as measured in cytotoxicity assays, from major histocompatibility complex (MHC) class I-restricted presentation to CD8+ T cells. This led to the concept of EBNA1 as an immunologically silent protein that although unique in being expressed in all EBV malignancies, could not be exploited as a CD8 target. Here, using CD8+ T cell clon... Abstract
Cited 80 times since 2003 (3.9 per year) source: EuropePMC
Journal of cell science, Volume 116, Issue Pt 19, 1 1 2003, Pages 3971-3984 Complex protein-DNA dynamics at the latent origin of DNA replication of Epstein-Barr virus. Ritzi M, Tillack K, Gerhardt J, Ott E, Humme S, Kremmer E, Hammerschmidt W, Schepers A
The sequential binding of the origin recognition complex (ORC), Cdc6p and the minichromosome maintenance proteins (MCM2-7) mediates replication competence at eukaryotic origins of DNA replication. The latent origin of Epstein-Barr virus, oriP, is a viral origin known to recruit ORC. OriP also binds EBNA1, a virally encoded protein that lacks any activity predicted to be required for replication initiation. Here, we used chromatin immunoprecipitation and chromatin binding to compare the cell-cycl... Abstract
Cited 121 times since 2003 (5.8 per year) source: EuropePMC
Proceedings of the National Academy of Sciences of the United States of America, Volume 100, Issue 19, 28 4 2003, Pages 10989-10994 The EBV nuclear antigen 1 (EBNA1) enhances B cell immortalization several thousandfold. Humme S, Reisbach G, Feederle R, Delecluse HJ, Bousset K, Hammerschmidt W, Schepers A
The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is one of the earliest viral proteins expressed after infection and is the only latent protein consistently expressed in viral-associated tumors. EBNA1's crucial role in viral DNA replication, episomal maintenance, and partitioning is well examined whereas its importance for the immortalization process and the tumorgenicity of EBV is unclear. To address these open questions, we generated, based on the maxi-EBV system, an EBNA1-deficient... Abstract
Cited 19 times since 2003 (0.9 per year) source: EuropePMC
Annals of vascular surgery, Volume 17, Issue 2, 6 1 2003, Pages 198-202 Complication registration in patients after peripheral arterial bypass surgery. Schepers A, Klinkert P, Vrancken Peeters MP, Breslau PJ
The objective of this study was to describe the kinds of complications and their incidence after peripheral vascular surgery of the lower limb, coding for causes and effect on the patient. In this prospective study, a standardized complication registration system was used at the Red Cross Hospital, The Hague. All patients (n = 373) receiving an infrainguinal bypass graft during the period January 1, 1996 to December 31, 1999 were included. All postoperative complications occurring during admissi... Abstract
Cited 107 times since 2003 (5 per year) source: EuropePMC
Journal of vascular surgery, Volume 37, Issue 1, 1 1 2003, Pages 149-155 Vein versus polytetrafluoroethylene in above-knee femoropopliteal bypass grafting: five-year results of a randomized controlled trial. Klinkert P, Schepers A, Burger DH, van Bockel JH, Breslau PJ
Objective: Controversy still exists whether polytetrafluoroethylene is equivalent to vein as bypass graft material for the above-knee femoropopliteal bypass. Therefore, a prospective randomized trial was performed to compare vein with polytetrafluoroethylene for femoropopliteal bypasses with the distal anastomosis above the knee. Methods: Between January 1993 and December 1996, 151 above-knee femoropopliteal bypasses were performed. The indications for operation were severe claudication in 120 c... Abstract
Cited 146 times since 2001 (6.4 per year) source: EuropePMC
The EMBO journal, Volume 20, Issue 16, 1 1 2001, Pages 4588-4602 Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus. Schepers A, Ritzi M, Bousset K, Kremmer E, Yates JL, Harwood J, Diffley JF, Hammerschmidt W
Epstein-Barr virus (EBV) replicates in its latent phase once per cell cycle in proliferating B cells. The latent origin of DNA replication, oriP, supports replication and stable maintenance of the EBV genome. OriP comprises two essential elements: the dyad symmetry (DS) and the family of repeats (FR), both containing clusters of binding sites for the transactivator EBNA1. The DS element appears to be the functional replicator. It is not yet understood how oriP-dependent replication is integrated... Abstract
Cited 34 times since 2001 (1.5 per year) source: EuropePMC
Journal of molecular biology, Volume 308, Issue 4, 1 1 2001, Pages 597-608 Mutational analysis of conserved sequence motifs in the budding yeast Cdc6 protein. Schepers A, Diffley JF
The Cdc6 protein is required to load a complex of Mcm2-7 family members (the MCM complex) into prereplicative complexes at budding yeast origins of DNA replication. Cdc6p is a member of the AAA(+) superfamily of proteins, which includes the prokaryotic and eukaryotic clamp loading proteins. These proteins share a number of conserved regions of homology and a common three-dimensional architecture. Two of the conserved sequence motifs are the Walker A and B motifs that are involved in nucleotide m... Abstract
Cited 63 times since 1996 (2.3 per year) source: EuropePMC
Virology, Volume 220, Issue 2, 1 1 1996, Pages 367-376 Activation of oriLyt, the lytic origin of DNA replication of Epstein-Barr virus, by BZLF1. Schepers A, Pich D, Hammerschmidt W
oriLyt, the cis-acting element of the lytic origin of DNA replication of Epstein-Barr virus, is activated by the viral transactivator BZLF1 which belongs to the extended bZIP class of transcription factors. Seven binding sites for BZLF1, so-called ZRE sites, are located within oriLyt. By mutational analysis of individual ZRE sites, we found that lytic DNA replication is dependent on only four of these sites which colocate with the promoter of the BHLF1 gene. The remaining three ZRE sites distal... Abstract
Cited 91 times since 1993 (3 per year) source: EuropePMC
The EMBO journal, Volume 12, Issue 10, 1 1 1993, Pages 3921-3929 A transcription factor with homology to the AP-1 family links RNA transcription and DNA replication in the lytic cycle of Epstein-Barr virus. Schepers A, Pich D, Hammerschmidt W
oriLyt, the lytic origin of DNA replication of Epstein-Barr virus (EBV), ensures viral DNA amplification during the productive or lytic phase of the virus' life cycle. To understand the contribution of cis- and transacting elements involved in DNA replication of oriLyt, a detailed mutational analysis was undertaken which defined BZLF1, a viral transcriptional activator, as an essential replication factor. The BZLF1 protein belongs to the extended fos/jun family of transcription factors and... Abstract
Cited 58 times since 1993 (1.9 per year) source: EuropePMC
Journal of virology, Volume 67, Issue 7, 1 1 1993, Pages 4237-4245 cis-acting elements in the lytic origin of DNA replication of Epstein-Barr virus. Schepers A, Pich D, Mankertz J, Hammerschmidt W
oriLyt, the cis-acting element of Epstein-Barr virus, mediates viral DNA replication in the lytic phase of the virus's life cycle. Oligonucleotide-directed in vitro mutagenesis of oriLyt plasmids allowed the identification of two noncontiguous components within the complex structure of oriLyt. Both components were indispensable for DNA replication of this origin. The upstream component colocalized with the promoter of the viral BHLF1-encoding gene, and mutants affecting DNA replication affe... Abstract
Cited 3 times since 1991 (0.1 per year) source: EuropePMC
New York state journal of medicine, Volume 91, Issue 5, 1 1 1991, Pages 196-200 A retrospective analysis of the metabolic status of stone formers in the New York City metropolitan areas. Hatch M, Schepers A, Grunberger I, Godec CJ
Eighty stone-forming patients were evaluated as outpatients for risk factors involved in stone formation. Analyses of the major stone risk parameters in the 24-hour collections indicated low volume (less than 2L) in about 79% of these patients. Forty percent of all patients were found to be hypocitraturic, 30% were hyperoxaluric, 18% were hypercalciuric, and 13% were hyperuricosuric. Sixty-one percent of the patients had a normal calcium load study; of these, four patients were uric acid stone f... Abstract
Cited 3 times since 1988 (0.1 per year) source: EuropePMC
Journal of pharmaceutical and biomedical analysis, Volume 6, Issue 6-8, 1 1 1988, Pages 895-902 Evaluation of sample work-up methods and internal standards for the determination of catecholamines in urine by HPLC with electrochemical detection. Claeys M, Schepers A, Dillen L, De Potter WP
Two sample work-up methods: (I) one consisting of adsorption of the catecholamines onto alumina followed by ion pair extraction and (II) another consisting of isolation by cation exchange and subsequent adsorption onto alumina, have been evaluated for the assay of urinary catecholamines by means of HPLC with electrochemical detection. With the aim of achieving high precision, two internal standards, i.e. dihydroxybenzylamine and epinine, have been compared. The results indicate that clean HPLC c... Abstract
Archiv der Pharmazie und Berichte der Deutschen Pharmazeutischen Gesellschaft, Volume 296, 1 1 1963, Pages 650-660 [SYNTHESIS OF 3 ISOMERIC VERATRUM DERIVATIVES OVER TETRAHYDROISOQUINOLINE AS AN INTERMEDIATE PRODUCT]. KNABE J, MATHEWS H, SCHEPERS A
Cited 10 times since 1962 (0.2 per year) source: EuropePMC
Archiv der Pharmazie und Berichte der Deutschen Pharmazeutischen Gesellschaft, Volume 295/67, 1 1 1962, Pages 481-488 [Dehydration of tertiary amines with Hg(II)-ethylenediaminetetra-acetate. 5. 1-Paired substituted N-methyl-6,7,-dimethoxy-1,2,3,4-tetrahydroisoquinoline]. KNABE J, SCHEPERS A