Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
238 results
Cited 19 times since 1995 (0.6 per year) source: EuropePMC
The American journal of cardiovascular pathology, Volume 5, Issue 1, 1 1 1995, Pages 27-31 Polyploidy of myocyte nuclei in pressure overloaded human hearts: a flow cytometric study in left and right ventricular myocardium. Vliegen HW, Eulderink F, Bruschke AV, van der Laarse A, Cornelisse CJ
Increased heart weight is known to be associated with polyploidy of myocardial nuclei. However, the relation between polyploidy and myocardial hypertrophy at a cellular level, i.e. myocyte hypertrophy, has not yet been investigated. The present study was conducted to reveal this relation in different regions in the heart using two recently developed techniques, i.e. flow cytometry to measure polyploidy and microscopic morphometry to measure myocyte dimensions in fixed tissue sections. We investi... Abstract
Cited 3 times since 1993 (0.1 per year) source: EuropePMC
Catheterization and cardiovascular diagnosis, Volume 29, Issue 4, 1 1 1993, Pages 283-284 Nineteen years survival after occlusion of the left main coronary artery by virtue of an anomalous septal branch. Vliegen HW, Cats VM
This case presents a patient who survived 19 years with an occluded left main coronary artery as a result of collateral flow to the anterior wall from an anomalous septal branch arising from the right coronary artery. The case illustrates that minor variations in coronary anatomy may have important effects on the prognosis of a patient. Abstract
Cited 2 times since 1993 (0.1 per year) source: EuropePMC
Journal of cardiovascular pharmacology, Volume 21, Issue 4, 1 1 1993, Pages 552-559 Comparison of diltiazem standard formulation and diltiazem controlled release in patients with stable angina pectoris: a randomized, double-blind, cross-over, multicenter study. Vliegen HW, van der Wall EE, Kragten JA, Holwerda NJ, Schenkel WM, Muijs van de Moer WM, ten Kate JB, Mulder PG, Bruschke AV
In a randomized, double-blind, cross-over, multicenter study with a placebo run-in phase, the efficacy and safety of two oral formulations of diltiazem, standard three or four times daily (t.i.d. or q.i.d.) and controlled release twice daily (b.i.d.), were compared in 49 patients with stable angina pectoris. ST-segment depression at maximum exercise 12 h after tablet intake was less frequently observed with diltiazem controlled release than with standard diltiazem (34 of 49, 69% vs. 43 of 49, 88... Abstract
Cited 7 times since 1992 (0.2 per year) source: EuropePMC
European journal of nuclear medicine, Volume 19, Issue 9, 1 1 1992, Pages 783-789 The clinical impact of thallium-201 reinjection scintigraphy for detection of myocardial viability. Kuijper AF, Vliegen HW, van der Wall EE, Oosterhuis WP, Zwinderman AH, van Eck-Smit BL, Niemeyer MG, Pauwels EK
In a clinical study, the value of thallium-201 reinjection was studied in 139 patients with suspected or known coronary artery disease who showed one or more persistent defects after conventional stress-redistribution imaging. Fifty-nine (42%) patients had sustained a Q-wave myocardial infarction. Sixty-eight (49%) patients showed a reversible defect in at least one myocardial segment at redistribution, while 71 (51%) had persistent defects only. Following reinjection additional segmental fillin... Abstract
Cited 98 times since 1991 (3 per year) source: EuropePMC
European heart journal, Volume 12, Issue 4, 1 1 1991, Pages 488-494 Myocardial changes in pressure overload-induced left ventricular hypertrophy. A study on tissue composition, polyploidization and multinucleation. Vliegen HW, van der Laarse A, Cornelisse CJ, Eulderink F
Morphological changes in human myocardium associated with pressure overload-induced left ventricular hypertrophy were studied in 22 normal and 21 hypertrophic hearts obtained at autopsy. Samples were obtained from the left lateral ventricular wall, half way between the apex and the base. Myocyte dimensions, polyploidization, multinucleation and relative volume fractions were studied. Regression analysis in relation to indexed heart weight yielded statistically significant correlation coefficient... Abstract
Journal of cardiovascular pharmacology, Volume 18 Suppl 9, 1 1 1991, Pages S68-72 Improvement of left ventricular function in silent ischemia following myocardial infarction, after administration of diltiazem. van der Wall EE, Niemeyer MG, Vliegen HW, Manger Cats V, Blokland JA, Pauwels EK, Bruschke AV
In a group of 72 patients with acute myocardial infarction who underwent a maximal symptom-limited predischarge exercise test in conjunction with radionuclide angiography, 25 (35%) showed greater than 1 mm asymptomatic ST-T-segment depression during exercise. All 25 patients underwent repeated exercise radionuclide angiography 2 days later, 2 h after oral intake of 120 mg diltiazem. Double product was not significantly different before and after diltiazem both at rest and during exercise. Maxima... Abstract
Cited 4 times since 1991 (0.1 per year) source: EuropePMC
Journal of cardiovascular pharmacology, Volume 18 Suppl 9, 1 1 1991, Pages S55-60 Long-term efficacy of diltiazem controlled release versus metoprolol in patients with stable angina pectoris. Vliegen HW, van der Wall EE, Niemeyer MG, Holwerda NJ, Bernink PJ, de Weerd P, Bosma AH, van der Wieken LR, Timmermans AJ, Molhoek GP
In a randomized, double-blind, multicenter study, the efficacy of diltiazem controlled-release (CR) 120 mg b.i.d. was compared with metoprolol 100 mg b.i.d. in 56 patients with stable exertional angina pectoris. Fifty-one patients (28 receiving diltiazem CR, 23 receiving metoprolol), completed a follow-up period of 8 weeks. Thirty-nine patients (20 receiving diltiazem CR, 19 receiving metoprolol), completed a follow-up period of 32 weeks. Maximal exercise testing was performed at baseline and af... Abstract
Cited 21 times since 1990 (0.6 per year) source: EuropePMC
Journal of molecular and cellular cardiology, Volume 22, Issue 9, 1 1 1990, Pages 987-998 Altered calcium handling at normal contractility in hypertrophied rat heart. Schouten VJ, Vliegen HW, van der Laarse A, Huysmans HA
Left or right ventricular hypertrophy was induced by banding of the aorta or pulmonary artery in different groups of rats. After 5 to 10 weeks the degree of hypertrophy was about 15% in left and 80-160% in right ventricles, as determined by weight of the ventricle or by myocyte size. Action potentials and force-interval relationships were measured in papillary muscles isolated from either ventricle. As compared to muscles from control and SHAM-operated rats, hypertrophied papillary muscles showe... Abstract
Cited 10 times since 1990 (0.3 per year) source: EuropePMC
Comparative biochemistry and physiology. A, Comparative physiology, Volume 95, Issue 1, 1 1 1990, Pages 109-114 Different response of cellular DNA content to cardiac hypertrophy in human and rat heart myocytes. Vliegen HW, Bruschke AV, Van der Laarse A
1. Rat and human heart myocytes adapt to overload-induced hypertrophy differently. 2. Human myocyte nuclei respond with polyploidization and multinucleation, thus increasing the DNA content per myocyte from 20 to 40 pg. As a result, nuclear DNA content per 10,000 microns3 of cell volume decreases from 12 to 10 pg. 3. In rat hearts with aortic constriction nuclear DNA content remains constant (13 pg), and the DNA content per 10,000 microns3 of myocyte volume falls from 9 to 6 pg. 4. We hypothesiz... Abstract
Cited 54 times since 1989 (1.5 per year) source: EuropePMC
Pathology, research and practice, Volume 184, Issue 6, 1 1 1989, Pages 577-581 Changes in nonmyocyte tissue composition associated with pressure overload of hypertrophic human hearts. Huysman JA, Vliegen HW, Van der Laarse A, Eulderink F
To investigate whether pressure-overloaded left ventricular hypertrophy is associated with changes in tissue composition of left ventricular subendocardial (ENDO) and subepicardial (EPI) myocardium, we studied post-mortem 19 normal hearts, 17 hearts of patients with systemic hypertension, and 5 hearts of patients with aortic stenosis. Coronary artery disease was present in 9 hearts of the hypertension group and all 5 hearts of the aortic stenosis group. By means of a grid-point method, volume pe... Abstract
Cited 3 times since 1989 (0.1 per year) source: EuropePMC
Cardiovascular research, Volume 23, Issue 4, 1 1 1989, Pages 308-314 Comparison of myocardial changes between pressure induced hypertrophy and normal growth in the rat heart. van der Laarse A, Vliegen HW, van der Nat KH, Hollaar L, Egas JM, Swier GP, van den Broek AJ
To evaluate differences in tissue composition between hearts with pressure overload hypertrophy and normal hearts of comparable weight, 30 rat hearts with aortic constriction of 4, 10 and 30 days, and nine hearts of sham operated controls were studied. Surgery was performed at age 70 days. Morphometric analysis of myocardial tissue sections revealed (1) myocyte hypertrophy in left ventricular myocardium of hypertrophic hearts was proportional to heart weight, and in normal growth myocyte volume... Abstract
Cited 4 times since 1989 (0.1 per year) source: EuropePMC
European journal of clinical investigation, Volume 19, Issue 2, 1 1 1989, Pages 192-200 Myocardial (iso)enzyme activities, DNA concentration and nuclear polyploidy in hearts of patients operated upon for congenital heart disease, and in normal and hypertrophic adult human hearts at autopsy. van der Laarse A, Hollaar L, Vliegen HW, Egas JM, Dijkshoorn NJ, Cornelisse CJ, Bogers AJ, Quaegebeur JM
To investigate biochemical characteristics of hypertrophic myocardium of young and adult humans, we analysed myocardial biopsies obtained from 28 mainly young patients undergoing cardiac surgery for congenital heart disease and 41 autopsied hearts from 18 adult normal and 23 hypertrophic human subjects. Myocardial activities of the enzymes creatine kinase and lactate dehydrogenase were independent of age during childhood, but decreased significantly with hypertrophy at adult age. Myocyte nuclei... Abstract
Cited 2 times since 1988 (0.1 per year) source: EuropePMC
The Thoracic and cardiovascular surgeon, Volume 36, Issue 3, 1 1 1988, Pages 137-140 Assessment of hypertrophy in myocardial biopsies taken during correction of congenital heart disease. Bogers AJ, van der Laarse A, Vliegen HW, Quaegebeur JM, Hollaar L, Egas JM, Cornelisse CJ, Rohmer J, Huysmans HA
Myocardial biopsies were obtained from 27 patients undergoing corrective cardiac surgery for congenital heart disease. Normal hearts of 18 autopsied patients were used as reference. The biopsy material was assessed for desoxyribonucleic acid (DNA) concentration and ploidy profile of cell nuclei in order to quantitate myocardial hypertrophy at the time of operation. DNA-concentration decreased significantly with age (r = -0.76; p less than 0.001). Ploidy profile of myocardial nuclei correlated wi... Abstract
Cited 16 times since 1987 (0.4 per year) source: EuropePMC
Research communications in chemical pathology and pharmacology, Volume 57, Issue 1, 1 1 1987, Pages 15-32 Quantification of myocardial necrosis and cardiac hypertrophy in isoproterenol-treated rats. Knufman NM, van der Laarse A, Vliegen HW, Brinkman CJ
Administration of a single high dose or multiple low doses of isoproterenol (ISO) to rats induces myocardial necrosis and cardiac hypertrophy. To assess the extent of necrosis we measured myocardial activities of creatine kinase (CK) and aspartate aminotransferase (AST) in the first days after ISO-treatment. After a single high dose and multiple low doses of ISO, necrosis was 10% and 25%, respectively. To quantify the degree of hypertrophy we measured heart weight and myocyte volume, and calcula... Abstract
Cited 23 times since 1987 (0.6 per year) source: EuropePMC
Cardiovascular research, Volume 21, Issue 5, 1 1 1987, Pages 352-357 Morphometric quantification of myocyte dimensions validated in normal growing rat hearts and applied to hypertrophic human hearts. Vliegen HW, van der Laarse A, Huysman JA, Wijnvoord EC, Mentar M, Cornelisse CJ, Eulderink F
To obtain quantitative information on changes in myocyte size in hearts with increased myocardial mass light microscopical morphometry was used in fixed and stained myocardial tissue sections. A modification of the azan staining procedure made longitudinal (sarcolemma) and transverse (intercalated discs) boundaries visible with a contrast that allowed morphometric analysis. The method was validated in left ventricular tissue of rats ranging in age from 4 to 17 weeks. Myocyte width, cut surface a... Abstract
Cited 7 times since 1987 (0.2 per year) source: EuropePMC
Cardiovascular research, Volume 21, Issue 3, 1 1 1987, Pages 223-229 Relation between ventricular DNA content and number of myocytes and non-myocytes in hearts of normotensive and spontaneously hypertensive rats. van der Laarse A, Bloys van Treslong CH, Vliegen HW, Ricciardi L
Myocardial DNA concentration and ventricular weight were measured in the hearts of normal and spontaneously hypertensive rats varying in age from 1 day to 1 year and the ventricular DNA content calculated. A linear relation between ventricular DNA content and ventricular weight was observed in both rat strains. This linear relation was deduced theoretically, assuming that the number of ventricular non-myocytes per heart was proportional to ventricular weight and that the number of ventricular my... Abstract
Bollettino della Societa italiana di biologia sperimentale, Volume 62, Issue 1, 1 1 1986, Pages 89-93 [Mechanical and structural variations in the hypertrophic myocardium of trained swimming rats]. Ricciardi L, ter Keurs HE, vd Laarse A, Vliegen HW
Cited 8 times since 1986 (0.2 per year) source: EuropePMC
Histochemistry, Volume 84, Issue 4-6, 1 1 1986, Pages 348-354 Methodological aspects of flow cytometric analysis of DNA polyploidy in human heart tissue. Vliegen HW, Vossepoel AM, van der Laarse A, Eulderink F, Cornelisse CJ
The purpose of the study was to investigate the possibilities of flow cytometry (FCM) for the analysis of DNA polyploidy in human heart tissue. Suspensions of single nuclei were prepared with the detergent-trypsin procedure and stained with propidium iodide. A mathematical correction procedure was developed to correct for background and clumping. For diploid model populations of chicken and trout red blood cells this correction reduced artifactual fractions in the FCM DNA profile to less than 0.... Abstract