Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
25 results
Cited 33 times since 2007 (2 per year) source: EuropePMC
Tuberculosis (Edinburgh, Scotland), Volume 88, Issue 3, 26 4 2007, Pages 249-261 Double-blind randomized Phase I study comparing rdESAT-6 to tuberculin as skin test reagent in the diagnosis of tuberculosis infection. Arend SM, Franken WP, Aggerbeck H, Prins C, van Dissel JT, Thierry-Carstensen B, Tingskov PN, Weldingh K, Andersen P
Limited specificity of the tuberculin skin test incited the development of in vitro assays based on Mycobacterium tuberculosis-specific antigens such as ESAT-6 that are lacking in Bacillus Calmette Guérin (BCG). In animal studies, intradermal ESAT-6 was safe and induced specific skin test responses. The aim of the study was to assess the safety of intradermal recombinant dimer ESAT-6 (rdESAT-6) compared with tuberculin and to determine the human dose. The study design was a double-blind Phase I... Abstract
Cited 69 times since 2007 (4.1 per year) source: EuropePMC
Clinical and vaccine immunology : CVI, Volume 14, Issue 7, 16 3 2007, Pages 880-885 Discrepancy between Mycobacterium tuberculosis-specific gamma interferon release assays using short and prolonged in vitro incubation. Leyten EM, Arend SM, Prins C, Cobelens FG, Ottenhoff TH, van Dissel JT
The sensitivities of various gamma interferon release assays (IGRAs) for the detection of past latent Mycobacterium tuberculosis infection are not known. In this study, we aimed to assess the effects of various IGRA formats and in vitro incubation periods on test outcome. The results of the tuberculin skin test (TST) were compared with those of the QuantiFERON-TB Gold in-tube (QFT-GIT) test, an overnight enzyme-linked immunospot assay (ELISPOT), and a 6-day lymphocyte stimulation test (LST) by u... Abstract
Cited 23 times since 2007 (1.3 per year) source: EuropePMC
Clinical and vaccine immunology : CVI, Volume 14, Issue 4, 14 2 2007, Pages 477-480 Comparison of Mantoux and QuantiFERON TB Gold tests for diagnosis of latent tuberculosis infection in Army personnel. Franken WP, Timmermans JF, Prins C, Slootman EJ, Dreverman J, Bruins H, van Dissel JT, Arend SM
The tuberculin skin test (TST) was compared with QuantiFERON-TB Gold in-tube (QFT-GIT) test for the diagnosis of tuberculosis in non-Mycobacterium bovis BCG-vaccinated military personnel. Among subjects positive by TST, 44.4% of recruits were positive by QFT-GIT compared with 11.5% subjects tested after missions abroad, suggesting that most TST conversions in the latter group were caused by nontuberculous mycobacteria. Abstract
Cited 178 times since 2006 (9.9 per year) source: EuropePMC
Microbes and infection, Volume 8, Issue 8, 13 2 2006, Pages 2052-2060 Human T-cell responses to 25 novel antigens encoded by genes of the dormancy regulon of Mycobacterium tuberculosis. Leyten EM, Lin MY, Franken KL, Friggen AH, Prins C, van Meijgaarden KE, Voskuil MI, Weldingh K, Andersen P, Schoolnik GK, Arend SM, Ottenhoff TH, Klein MR
The dormancy (DosR) regulon of Mycobacterium tuberculosis is expressed in vitro during hypoxia and low-dose nitric oxide stimulation. Tubercle bacilli are thought to encounter these conditions in humans during latent infection. In this study, immune responses were evaluated to 25 most strongly induced DosR-regulon-encoded proteins, referred to as latency antigens. Proliferation assays were performed using M. tuberculosis-specific T-cell lines and peripheral blood mononuclear cells (PBMC) from tu... Abstract
Cited 11 times since 2006 (0.6 per year) source: EuropePMC
Journal of clinical microbiology, Volume 44, Issue 3, 1 1 2006, Pages 1197-1201 Use of enzyme-linked immunospot assay with Mycobacterium tuberculosis-specific peptides for diagnosis of recent infection with M. tuberculosis after accidental laboratory exposure. Leyten EM, Mulder B, Prins C, Weldingh K, Andersen P, Ottenhoff TH, van Dissel JT, Arend SM
This report of an accidental exposure to Mycobacterium tuberculosis in a microbiological laboratory illustrates the value of gamma interferon enzyme-linked immunospot assay using peptides of ESAT-6, CFP-10, TB37.6, and TB7.7 for the diagnosis of latent infection. In particular, positive responses to peptides 2 to 6 of TB37.6 were observed exclusively in recently infected persons. Abstract