Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
165 results
Cited 11 times since 1994 (0.4 per year) source: EuropePMC
Journal of molecular and cellular cardiology, Volume 26, Issue 5, 1 1 1994, Pages 639-648 The effect of sarcolemmal cholesterol content on the tolerance to anoxia in cardiomyocyte cultures. Bastiaanse EM, van der Valk-Kokshoorn LJ, Egas-Kenniphaas JM, Atsma DE, van der Laarse A
To determine whether the sarcolemmal-free cholesterol content influences the tolerance to anoxia in cultured neonatal rat cardiomyocytes, we modulated the free cholesterol content of the cultures, and determined the time course of anoxia-induced cell death. Incubation for 5 h with liposomes having a free cholesterol to phospholipid molar ratio of 2, 0.5 and 0, resulted in a change of cellular free cholesterol content by +54.7 +/- 5.8% (P < 0.001), by -5.2 +/- 6.3% (n.s.), and by -22.1 +/- 4.9... Abstract
Cited 10 times since 1994 (0.3 per year) source: EuropePMC
FEBS letters, Volume 343, Issue 2, 1 1 1994, Pages 151-154 Simvastatin-sodium delays cell death of anoxic cardiomyocytes by inhibition of the Na+/Ca2+ exchanger. Bastiaanse EM, Atsma DE, Kuijpers MM, Van der Laarse A
When incubated under anoxic conditions, cultured neonatal cardiomyocytes undergo cell necrosis. Simvastatin-sodium, the bioactive metabolite of simvastatin (a potent serum cholesterol-lowering drug), delayed the anoxia-induced myocyte necrosis in a dose-dependent manner. This beneficial effect of simvastatin-sodium could not be attributed to its cholesterol-lowering properties. We found that simvastatin-sodium, at concentrations of 20 and 50 microM, attenuated the rise in intracellular Ca2+ conc... Abstract
Cited 15 times since 1993 (0.5 per year) source: EuropePMC
Nuclear medicine and biology, Volume 20, Issue 7, 1 1 1993, Pages 825-833 The labeling of proteins and LDL with 99mTc: a new direct method employing KBH4 and stannous chloride. Pauwels EK, Welling MM, Feitsma RI, Atsma DE, Nieuwenhuizen W
A new direct labeling technique for proteins with technetium-99m (99mTc) has been developed and makes use of borohydride and stannous chloride. The method is simple and reproducible and gives a high labeling efficiency and high retention of biological activity for proteins, including polyclonal immunoglobulin (Ig), antifibrin monoclonal antibody, tissue type plasminogen activator, fibrinogen and low density lipoprotein (LDL). This method can be used in kit-format and takes about 20 min preparati... Abstract
Cited 6 times since 1993 (0.2 per year) source: EuropePMC
Arteriosclerosis and thrombosis : a journal of vascular biology, Volume 13, Issue 1, 1 1 1993, Pages 78-83 Potential of 99mTc-LDLs labeled by two different methods for scintigraphic detection of experimental atherosclerosis in rabbits. Atsma DE, Feitsma RI, Camps J, van't Hooft FM, van der Wall EE, Nieuwenhuizen W, Pauwels EK
In this study we evaluated two different 99mTc-labeling techniques to produce 99mTc-low density lipoprotein (99mTc-LDL) suitable for the scintigraphic delineation of experimental atherosclerotic lesions. The two methods are 1) a procedure that uses stannous chloride and sodium borohydride (borohydride method) and 2) a procedure that uses sodium dithionite as a reducing agent and that has been successfully applied in previous scintigraphic atherosclerosis detection (dithionite method). 99mTc-LDL... Abstract
Cited 10 times since 1991 (0.3 per year) source: EuropePMC
Journal of lipid research, Volume 32, Issue 1, 1 1 1991, Pages 173-181 Partial characterization of low density lipoprotein preparations isolated from fresh and frozen plasma after radiolabeling by seven different methods. Atsma DE, Kempen HJ, Nieuwenhuizen W, van 't Hooft FM, Pauwels EK
Four 99mTc and three 123I labeling methods were evaluated for their suitability to label low density lipoproteins (LDL) for the purpose of scintigraphic biodistribution studies. For 99mTc these methods were: direct incorporation in LDL of 99mTcO4- using sodium dithionite (dithionite method); a method using first N,N-dimethylformamide to prepare a 99mTc-complex reacting with LDL in a subsequent step (DMF method); a technique in which 99mTcO4- is first coupled to a diamide dithiolate derivative of... Abstract