Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
63 results
Cited 270 times since 2004 (13.5 per year) source: EuropePMC
Annals of the rheumatic diseases, Volume 63, Issue 4, 1 1 2004, Pages 373-381 Expression and activity of citrullinating peptidylarginine deiminase enzymes in monocytes and macrophages. Vossenaar ER, Radstake TR, van der Heijden A, van Mansum MA, Dieteren C, de Rooij DJ, Barrera P, Zendman AJ, van Venrooij WJ
Background: Antibodies directed to proteins containing the non-standard amino acid citrulline, are extremely specific for rheumatoid arthritis (RA). Peptidylcitrulline can be generated by post-translational conversion of arginine residues. This process, citrullination, is catalysed by a group of calcium dependent peptidylarginine deiminase (PAD) enzymes. Objective: To investigate the expression and activity of four isotypes of PAD in peripheral blood and synovial fluid cells of patients with RA.... Abstract
Cited 267 times since 2004 (13.2 per year) source: EuropePMC
Arthritis research & therapy, Volume 6, Issue 2, 5 1 2004, Pages R142-50 Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin. Vossenaar ER, Després N, Lapointe E, van der Heijden A, Lora M, Senshu T, van Venrooij WJ, Ménard HA
Antibodies directed to the Sa antigen are highly specific for rheumatoid arthritis (RA) and can be detected in approximately 40% of RA sera. The antigen, a doublet of protein bands of about 50 kDa, is present in placenta and in RA synovial tissue. Although it has been stated that the Sa antigen is citrullinated vimentin, experimental proof for this claim has never been published. In this study, we investigated the precise nature of the antigen. Peptide sequences that were obtained from highly pu... Abstract
Cited 47 times since 2003 (2.3 per year) source: EuropePMC
Molecular biology of the cell, Volume 15, Issue 2, 14 2 2003, Pages 611-624 Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes. Fouraux MA, Deneka M, Ivan V, van der Heijden A, Raymackers J, van Suylekom D, van Venrooij WJ, van der Sluijs P, Pruijn GJ
We describe the characterization of an 80-kDa protein cross-reacting with a monoclonal antibody against the human La autoantigen. The 80-kDa protein is a variant of rabip4 with an N-terminal extension of 108 amino acids and is expressed in the same cells. For this reason, we named it rabip4'. rabip4' is a peripheral membrane protein, which colocalized with internalized transferrin and EEA1 on early endosomes. Membrane association required the presence of the FYVE domain and was perturb... Abstract
Cited 158 times since 2003 (7.7 per year) source: EuropePMC
Arthritis and rheumatism, Volume 48, Issue 9, 1 1 2003, Pages 2489-2500 Citrullination of synovial proteins in murine models of rheumatoid arthritis. Vossenaar ER, Nijenhuis S, Helsen MM, van der Heijden A, Senshu T, van den Berg WB, van Venrooij WJ, Joosten LA
Objective: Antibodies directed to citrulline-containing proteins are highly specific for rheumatoid arthritis (RA) and can be detected in up to 80% of patients with RA. Citrulline is a nonstandard amino acid that can be incorporated into proteins only by posttranslational modification of arginine by peptidylarginine deiminase (PAD) enzymes. The objective of this study was to investigate the presence of anticitrulline antibodies, PAD enzymes, and citrullinated antigens in mouse models of both acu... Abstract
Cited 33 times since 2002 (1.5 per year) source: EuropePMC
RNA (New York, N.Y.), Volume 8, Issue 11, 1 1 2002, Pages 1428-1443 The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase. Fouraux MA, Kolkman MJ, Van der Heijden A, De Jong AS, Van Venrooij WJ, Pruijn GJ
The human La (SS-B) autoantigen is an abundantly expressed putative RNA chaperone, functioning in various intracellular processes involving RNA. To further explore the molecular mechanisms by which La functions in these processes, we performed large-scale immunoprecipitations of La from HeLa S100 extracts using the anti-La monoclonal antibody SW5. La-associated proteins were subsequently identified by sequence analysis. This approach allowed the identification of DDX15 as a protein interacting w... Abstract
Cited 5 times since 2002 (0.2 per year) source: EuropePMC
Immunology, Volume 106, Issue 3, 1 1 2002, Pages 336-342 Cross-reactivity of the anti-La monoclonal antibody SW5 with early endosome antigen 2. Fouraux MA, van der Heijden A, van Venrooij WJ, Pruijn GJ
Coimmunoprecipitation studies with SW5, a frequently used and specific mouse monoclonal antibody (mAb) directed against the human La autoantigen, led to the identification of a functionally unrelated 80 000 MW protein, designated early endosome antigen 2 (EEA2). EEA2 appeared to be directly targeted by mAb SW5. Because an RNA-binding domain, a structural element of La containing the SW5-epitope, was not discernable in the primary structure of EEA2, the SW5-epitope on EEA2 was determined. Coiled-... Abstract
Cited 14 times since 2001 (0.6 per year) source: EuropePMC
Molecular biology of the cell, Volume 12, Issue 11, 1 1 2001, Pages 3680-3689 Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association. van Eenennaam H, van der Heijden A, Janssen RJ, van Venrooij WJ, Pruijn GJ
The RNase MRP and RNase P ribonucleoprotein particles both function as endoribonucleases, have a similar RNA component, and share several protein subunits. RNase MRP has been implicated in pre-rRNA processing and mitochondrial DNA replication, whereas RNase P functions in pre-tRNA processing. Both RNase MRP and RNase P accumulate in the nucleolus of eukaryotic cells. In this report we show that for three protein subunits of the RNase MRP complex (hPop1, hPop4, and Rpp38) basic domains are respon... Abstract
Cited 45 times since 2001 (2 per year) source: EuropePMC
Pediatric cardiology, Volume 22, Issue 4, 1 1 2001, Pages 321-326 Acute renal insufficiency and renal replacement therapy after pediatric cardiopulmonary bypass surgery. Kist-van Holthe tot Echten JE, Goedvolk CA, Doornaar MB, van der Vorst MM, Bosman-Vermeeren JM, Brand R, van der Heijden AJ, Schoof PH, Hazekamp MG
The aim of the study was to investigate renal function and renal replacement therapy after cardiopulmonary bypass surgery in children. Patient characteristics (sex, age, diagnosis), operation type, and death were listed. The study was performed retrospectively using serum creatinine level before, and peak values after, cardiopulmonary bypass surgery for assessment of renal function. Of the children on renal replacement therapy, indication, efficacy, and complications were recorded. In a 5-year p... Abstract
Cited 16 times since 2001 (0.7 per year) source: EuropePMC
RNA (New York, N.Y.), Volume 7, Issue 5, 1 1 2001, Pages 741-752 Identification of a novel cis-acting RNA element involved in nuclear export of hY RNAs. Rutjes SA, Lund E, van der Heijden A, Grimm C, van Venrooij WJ, Pruijn GJ
Ro RNPs are small cytoplasmic RNA-protein complexes of unknown function that have been found in all metazoan cells studied so far. In human cells, Ro RNPs consist of one of four small RNA molecules, termed hY RNAs and at least two well-characterized proteins, Ro60 and La. In previous Xenopus laevis oocyte microinjection studies, we showed that an intact Ro60 binding site (Stem-loop 1) is a prerequisite for efficient nuclear export of hY1 RNA, whereas an intact La-binding site promotes nuclear re... Abstract
Cited 31 times since 2000 (1.3 per year) source: EuropePMC
Biochemistry, Volume 39, Issue 11, 1 1 2000, Pages 3023-3033 Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution. Broekhuis CH, Neubauer G, van der Heijden A, Mann M, Proud CG, van Venrooij WJ, Pruijn GJ
The La (SS-B) autoantigen is an evolutionarily conserved phosphoprotein which plays an important role, most likely as an RNA chaperone, in various processes, such as the biosynthesis and maturation of RNA polymerase III transcripts in the cell nucleus and (internal) initiation of translation in the cytoplasm. In this study, the phosphorylation state of this protein from human HeLa and HEp-2 cells was characterized by high-resolution two-dimensional IEF/SDS-PAGE analysis, and phosphorylation site... Abstract
Cited 73 times since 1999 (3 per year) source: EuropePMC
Cell death and differentiation, Volume 6, Issue 10, 1 1 1999, Pages 976-986 The La (SS-B) autoantigen, a key protein in RNA biogenesis, is dephosphorylated and cleaved early during apoptosis. Rutjes SA, Utz PJ, van der Heijden A, Broekhuis C, van Venrooij WJ, Pruijn GJ
In the past few years, a role for apoptotic processes in the development of autoimmune diseases has been suggested. An increasing number of cellular proteins, which are modified during apoptosis, has been described, and many of these proteins have been identified as autoantigens. We have studied the effects of apoptosis on the La protein in more detail and for the first time demonstrate that this autoantigen is rapidly dephosphorylated after the induction of apoptosis. Dephosphorylation of the L... Abstract
Cited 60 times since 1999 (2.4 per year) source: EuropePMC
The Journal of biological chemistry, Volume 274, Issue 35, 1 1 1999, Pages 24799-24807 Rapid nucleolytic degradation of the small cytoplasmic Y RNAs during apoptosis. Rutjes SA, van der Heijden A, Utz PJ, van Venrooij WJ, Pruijn GJ
We have investigated the fate of the RNA components of small ribonucleoprotein particles in apoptotic cells. We show that the cytoplasmic Ro ribonucleoprotein-associated Y RNAs are specifically and rapidly degraded during apoptosis via a caspase-dependent mechanism. This is the first study describing the selective degradation of a specific class of small structural RNA molecules in apoptotic cells. Cleavage and subsequent truncation of Y RNAs was observed upon exposure of cells to a variety of a... Abstract
Cited 17 times since 1999 (0.7 per year) source: EuropePMC
Journal of chromatography. A, Volume 844, Issue 1-2, 1 1 1999, Pages 295-305 Trace-level determination of polar flavour compounds in butter by solid-phase extraction and gas chromatography-mass spectrometry. Adahchour M, Vreuls RJ, van der Heijden A, Brinkman UA
Volatile compounds are responsible for the aromas of butter. A simple technique for the determination of these components is described which is based on solid-phase extraction (SPE) after melting of the butter and separation of the aqueous phase from the fat. Volatile flavours present in the water fraction are collected by off-line SPE on cartidges packed with a copolymer sorbent. After desorption with 500 microliters of methyl acetate, 1-microliter aliquots are quantified and/or identified by g... Abstract
Cited 3 times since 1996 (0.1 per year) source: EuropePMC
Lung cancer (Amsterdam, Netherlands), Volume 15, Issue 1, 1 1 1996, Pages 131-135 Actinomycosis, a sheep in wolves' clothes. Neijens V, van Heerde P, van der Heijden A, Baas P
We present a patient referred for radiotherapy for a presumed pulmonary malignancy, who was found to suffer from an actinomycotic infection. This case illustrates the importance of early consideration of actinomycosis when diagnostic methods are negative for malignancy or specific chestwall and bony lesions are observed. Abstract
Cited 3 times since 1995 (0.1 per year) source: EuropePMC
Laboratory animals, Volume 29, Issue 4, 1 1 1995, Pages 459-463 Spleen pigmentation in young C57BL mice is caused by accumulation of melanin. van der Heijden A, van Dijk JE, Lemmens AG, Beynen AC
It has been previously reported that in 2 C57BL mouse sublines a dark pigmentation of the cranial part of the spleen occurs in up to 30% of the animals within the populations. It was not clear whether this discoloration is caused by melanosis, lipofuscinosis or haemosiderosis. With the use of light and electron microscopy of stained spleen sections, we identified the pigment in 14 out of 60 C57BL mice aged 8-10 wks. In the mice with pigmented spleens there was accumulation of melanin, predominan... Abstract
Cited 1 times since 1994 (0 per year) source: EuropePMC
The British journal of nutrition, Volume 72, Issue 4, 1 1 1994, Pages 567-577 Dietary fructose v. glucose in rats raises urinary excretion, true absorption and ileal solubility of magnesium but decreases magnesium retention. van der Heijden A, van den Berg GJ, Lemmens AG, Beynen AC
Apparent Mg absorption, that is Mg intake minus faecal excretion, was found to be greater in rats fed on diets containing fructose instead of glucose. This effect of fructose was accompanied by enhanced urinary excretion and diminished retention of Mg. True Mg absorption was then determined with the use of oral and intraperitoneal administration of tracer doses of 28 Mg. True Mg absorption was significantly greater in rats fed on fructose. There was no significant effect of fructose v. glucose o... Abstract
Cited 18 times since 1994 (0.6 per year) source: EuropePMC
Electroencephalography and clinical neurophysiology, Volume 93, Issue 3, 1 1 1994, Pages 163-168 Fasciculation potentials in foot and leg muscles of healthy young adults. Van der Heijden A, Spaans F, Reulen J
The occurrence of fasciculation potentials (FPs) was studied in healthy subjects aged 18-25. In 25 males and 25 females 3 intrinsic foot muscles, the tibialis anterior and the gastrocnemius muscles on both sides were monitored with surface electrodes for 2 min periods. Only potentials with a peak-to-peak amplitude of at least 50 microV were counted. The number of FPs per minute (FPs/min) was significantly higher in the abductor hallucis (AH) and significantly lower in the tibialis anterior as co... Abstract
Cited 6 times since 1993 (0.2 per year) source: EuropePMC
Biological trace element research, Volume 38, Issue 2, 1 1 1993, Pages 107-115 Dietary fructose vs glucose lowers copper solubility in the digesta in the small intestine of rats. Van den Berg GJ, Yu S, Van der Heijden A, Lemmens AG, Beynen AC
The hypothesis was tested that dietary fructose vs glucose lowers copper solubility in the digesta in the small intestine of rats, which in turn causes a decreased copper absorption. Male rats were fed adequate-copper (5 mg Cu/kg) diets containing either fructose or glucose (709.4 g monosaccharide/kg) for a period of 5 wk. Fructose vs glucose significantly lowered copper concentrations in plasma and the liver, but did not alter hepatic copper mass. Fructose feeding resulted in a significantly le... Abstract
Cited 2 times since 1993 (0.1 per year) source: EuropePMC
Nutricion hospitalaria, Volume 8, Issue 5, 1 1 1993, Pages 279-287 [Effect of increasing protein ingestion on the nitrogen balance of mechanically ventilated critically ill patients receiving total parenteral nutrition]. van der Heijden A, Verbeek MJ, Schreurs VV, Akkermans LM, Vos A
The amount of protein recommended to minimise N loss in critically ill patients receiving total parenteral nutrition (TPN) varies in the literature. Therefore, we studied the effect of increased protein intake on the N balance, administering TPN with either 1.2 g protein/kg/day (low N diet) or 1.8 g protein/kg/day (high N diet). Fifteen mechanically ventilated critically ill patients were studied in a surgical intensive care unit. After at least two days of standard TPN, patients were randomly a... Abstract
Clinical nutrition (Edinburgh, Scotland), Volume 11, Issue 6, 1 1 1992, Pages 376 Correspondence. Verbeek MJ, van der Heijden A, Schreurs VV, Akkermans LM, Vos A