Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
110 results
Cited 14 times since 2008 (0.9 per year) source: EuropePMC
PloS one, Volume 3, Issue 8, 29 5 2008, Pages e3084 Targeted chromosomal insertion of large DNA into the human genome by a fiber-modified high-capacity adenovirus-based vector system. Gonçalves MA, Holkers M, van Nierop GP, Wieringa R, Pau MG, de Vries AA
A prominent goal in gene therapy research concerns the development of gene transfer vehicles that can integrate exogenous DNA at specific chromosomal loci to prevent insertional oncogenesis and provide for long-term transgene expression. Adenovirus (Ad) vectors arguably represent the most efficient delivery systems of episomal DNA into eukaryotic cell nuclei. The most advanced recombinant Ads lack all adenoviral genes. This renders these so-called high-capacity (hc) Ad vectors less cytotoxic/imm... Abstract
Cited 88 times since 2008 (5.5 per year) source: EuropePMC
Circulation research, Volume 103, Issue 2, 12 2 2008, Pages 167-176 Forced alignment of mesenchymal stem cells undergoing cardiomyogenic differentiation affects functional integration with cardiomyocyte cultures. Pijnappels DA, Schalij MJ, Ramkisoensing AA, van Tuyn J, de Vries AA, van der Laarse A, Ypey DL, Atsma DE
Alignment of cardiomyocytes (CMCs) contributes to the anisotropic (direction-related) tissue structure of the heart, thereby facilitating efficient electrical and mechanical activation of the ventricles. This study aimed to investigate the effects of forced alignment of stem cells during cardiomyogenic differentiation on their functional integration with CMC cultures. Labeled neonatal rat (nr) mesenchymal stem cells (nrMSCs) were allowed to differentiate into functional heart muscle cells in dif... Abstract
Cited 26 times since 2008 (1.6 per year) source: EuropePMC
Molecular therapy : the journal of the American Society of Gene Therapy, Volume 16, Issue 4, 4 1 2008, Pages 741-748 Genetic complementation of human muscle cells via directed stem cell fusion. Gonçalves MA, Swildens J, Holkers M, Narain A, van Nierop GP, van de Watering MJ, Knaän-Shanzer S, de Vries AA
Duchenne muscular dystrophy (DMD) is caused by mutations in the X chromosome-linked DMD gene, which encodes the sarcolemma-stabilizing protein-dystrophin. Initial attempts at DMD therapy deployed muscle progenitor cells from healthy donors. The utilization of these cells is, however, hampered by their immunogenicity, while those from DMD patients are scarce and display limited ex vivo replication. Nonmuscle cells with myogenic capacity may offer valuable alternatives especially if, to allow auto... Abstract
Cited 41 times since 2008 (2.5 per year) source: EuropePMC
Stem cells (Dayton, Ohio), Volume 26, Issue 4, 17 3 2008, Pages 1083-1093 Forced myocardin expression enhances the therapeutic effect of human mesenchymal stem cells after transplantation in ischemic mouse hearts. Grauss RW, van Tuyn J, Steendijk P, Winter EM, Pijnappels DA, Hogers B, Gittenberger-De Groot AC, van der Geest R, van der Laarse A, de Vries AA, Schalij MJ, Atsma DE
Human mesenchymal stem cells (hMSCs) have only a limited differentiation potential toward cardiomyocytes. Forced expression of the cardiomyogenic transcription factor myocardin may stimulate hMSCs to acquire a cardiomyogenic phenotype, thereby improving their possible therapeutic potential. hMSCs were transduced with green fluorescent protein (GFP) and myocardin (hMSC(myoc)) or GFP and empty vector (hMSC). After coronary ligation in immune-compromised NOD/scid mice, hMSC(myoc) (n = 10), hMSC (n... Abstract
Cited 18 times since 2007 (1.1 per year) source: EuropePMC
Circulation, Volume 116, Issue 18, 15 3 2007, Pages 2018-2028 Resynchronization of separated rat cardiomyocyte fields with genetically modified human ventricular scar fibroblasts. Pijnappels DA, van Tuyn J, de Vries AA, Grauss RW, van der Laarse A, Ypey DL, Atsma DE, Schalij MJ
Background: Nonresponse to cardiac resynchronization therapy is associated with the presence of slow or nonconducting scar tissue. Genetic modification of scar tissue, aimed at improving conduction, may be a novel approach to achieve effective resynchronization. Therefore, the feasibility of resynchronization with genetically modified human ventricular scar fibroblasts was studied in a coculture model. Methods and results: An in vitro model was used to study the effects of forced expression of t... Abstract
Cited 37 times since 2007 (2.2 per year) source: EuropePMC
American journal of physiology. Heart and circulatory physiology, Volume 293, Issue 4, 20 3 2007, Pages H2438-47 Mesenchymal stem cells from ischemic heart disease patients improve left ventricular function after acute myocardial infarction. Grauss RW, Winter EM, van Tuyn J, Pijnappels DA, Steijn RV, Hogers B, van der Geest RJ, de Vries AA, Steendijk P, van der Laarse A, Gittenberger-de Groot AC, Schalij MJ, Atsma DE
Mesenchymal stem cells (MSCs) from healthy donors improve cardiac function in experimental acute myocardial infarction (AMI) models. However, little is known about the therapeutic capacity of human MSCs (hMSCs) from patients with ischemic heart disease (IHD). Therefore, the behavior of hMSCs from IHD patients in an immune-compromised mouse AMI model was studied. Enhanced green fluorescent protein-labeled hMSCs from IHD patients (hMSC group: 2 x 10(5) cells in 20 microl, n = 12) or vehicle only (... Abstract
Cited 28 times since 2007 (1.7 per year) source: EuropePMC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Volume 21, Issue 12, 19 3 2007, Pages 3369-3379 Fibroblasts from human postmyocardial infarction scars acquire properties of cardiomyocytes after transduction with a recombinant myocardin gene. van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der Velde-van Dijke I, Knaän-Shanzer S, van der Laarse A, Schalij MJ, Atsma DE
Myocardial scar formation impairs heart function by inducing cardiac remodeling, decreasing myocardial compliance, and compromising normal electrical conduction. Conversion of myocardial scar fibroblasts (MSFs) into (functional) cardiomyocytes may be an effective alternative treatment to limit loss of cardiac performance after myocardial injury. In this study, we investigated whether the phenotype of MSFs can be modified by gene transfer into cells with properties of cardiomyocytes. To this end,... Abstract
Cited 4 times since 2006 (0.2 per year) source: EuropePMC
BioTechniques, Volume 41, Issue 6, 1 1 2006, Pages 711-713 Modular and excisable molecular switch for the induction of gene expression by the yeast FLP recombinase. Holkers M, De Vries AA, Gonçalves MA
Cited 25 times since 2006 (1.4 per year) source: EuropePMC
Journal of cell science, Volume 119, Issue Pt 20, 26 4 2006, Pages 4247-4256 Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells. Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaän-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
Apoptosis is fundamental to the regulation of homeostasis of stem cells in vivo. Whereas the pathways underlying the molecular and biochemical details of nuclear breakdown that accompanies apoptosis have been elucidated, the precise nature of nuclear reorganization that precedes the demolition phase is not fully understood. Here, we expressed an inducible caspase-8 in human mesenchymal stem cells, and quantitatively followed the early changes in nuclear organization during apoptosis. We found th... Abstract
Cited 105 times since 2006 (6 per year) source: EuropePMC
Stem cells (Dayton, Ohio), Volume 25, Issue 2, 21 3 2006, Pages 271-278 Epicardial cells of human adults can undergo an epithelial-to-mesenchymal transition and obtain characteristics of smooth muscle cells in vitro. van Tuyn J, Atsma DE, Winter EM, van der Velde-van Dijke I, Pijnappels DA, Bax NA, Knaän-Shanzer S, Gittenberger-de Groot AC, Poelmann RE, van der Laarse A, van der Wall EE, Schalij MJ, de Vries AA
Myocardial and coronary development are both critically dependent on epicardial cells. During cardiomorphogenesis, a subset of epicardial cells undergoes an epithelial-to-mesenchymal transition (EMT) and invades the myocardium to differentiate into various cell types, including coronary smooth muscle cells and perivascular and cardiac interstitial fibroblasts. Our current knowledge of epicardial EMT and the ensuing epicardium-derived cells (EPDCs) comes primarily from studies of chick and mouse... Abstract
Cited 3 times since 2006 (0.2 per year) source: EuropePMC
Virus genes, Volume 33, Issue 1, 1 1 2006, Pages 59-68 The intraleader AUG nucleotide sequence context is important for equine arteritis virus replication. Archambault D, Kheyar A, de Vries AA, Rottier PJ
The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the subgenomic mRNA7 behind a T7 promoter. Capped RNA transcribed from this construct was shown to direct, in an in vitro translation system, the synthesis of leader peptide as well as N... Abstract
Cited 45 times since 2006 (2.5 per year) source: EuropePMC
Cardiovascular research, Volume 72, Issue 2, 29 5 2006, Pages 282-291 Progressive increase in conduction velocity across human mesenchymal stem cells is mediated by enhanced electrical coupling. Pijnappels DA, Schalij MJ, van Tuyn J, Ypey DL, de Vries AA, van der Wall EE, van der Laarse A, Atsma DE
Objective: The purpose of the study was to investigate the development of electrical transmission across human adult bone marrow-derived mesenchymal stem cells (hMSCs) during long-term co-incubation with cardiomyocytes (CMCs). Methods: Neonatal rat CMCs were cultured in multi-electrode array dishes. A conduction block was induced by creating a central acellular channel, yielding two asynchronously beating CMC fields. Enhanced green fluorescent protein (eGFP)-labeled hMSCs from ischemic heart dis... Abstract
Cited 60 times since 2006 (3.3 per year) source: EuropePMC
Reviews in medical virology, Volume 16, Issue 3, 1 1 2006, Pages 167-186 Adenovirus: from foe to friend. Gonçalves MA, de Vries AA
Human adenoviruses (HAdVs) can cause mild respiratory, gastrointestinal, urogenital and ocular disease. Knowledge about HAdVs has been expanding for more than five decades putting them amongst the most-studied viruses. This continued interest stems, to a great extent, from the fact that these double-stranded DNA viruses have proven to be a versatile tool to probe the basic phenomena of eukaryotic cells. HAdV research has led to the discovery of, for instance, RNA splicing and greatly contributed... Abstract
Cited 29 times since 2006 (1.6 per year) source: EuropePMC
Molecular therapy : the journal of the American Society of Gene Therapy, Volume 13, Issue 5, 26 4 2006, Pages 976-986 Transduction of myogenic cells by retargeted dual high-capacity hybrid viral vectors: robust dystrophin synthesis in duchenne muscular dystrophy muscle cells. Gonçalves MA, Holkers M, Cudré-Mauroux C, van Nierop GP, Knaän-Shanzer S, van der Velde I, Valerio D, de Vries AA
Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene (DMD), making it amenable to gene- or cell-based therapies. Another possible treatment entails the combination of both principles by transplantation of autologous myogenic cells after their genetic complementation. This approach requires efficient and stable transduction of these cells with recombinant DMD. Recently, we generated a dual high-capacity (hc) adenovirus (Ad)-adeno-associated virus (AAV) hybrid vector (HV... Abstract
Cited 48 times since 2005 (2.6 per year) source: EuropePMC
Human molecular genetics, Volume 15, Issue 2, 1 1 2005, Pages 213-221 Human mesenchymal stem cells ectopically expressing full-length dystrophin can complement Duchenne muscular dystrophy myotubes by cell fusion. Gonçalves MA, de Vries AA, Holkers M, van de Watering MJ, van der Velde I, van Nierop GP, Valerio D, Knaän-Shanzer S
Duchenne muscular dystrophy (DMD) is the most prevalent inheritable muscle disease. It is caused by mutations in the approximately 2.5-megabase dystrophin (Dys) encoding gene. Therapeutic attempts at DMD have relied on injection of allogeneic Dys-positive myoblasts. The immune rejection of these cells and their limited availability have prompted the search for alternative therapies and sources of myogenic cells. Stem cell-based gene therapy aims to restore tissue function by the transplantation... Abstract
Cited 38 times since 2005 (2.1 per year) source: EuropePMC
Stem cells (Dayton, Ohio), Volume 23, Issue 10, 1 1 2005, Pages 1598-1607 Endowing human adenovirus serotype 5 vectors with fiber domains of species B greatly enhances gene transfer into human mesenchymal stem cells. Knaän-Shanzer S, van de Watering MJ, van der Velde I, Gonçalves MA, Valerio D, de Vries AA
Bone marrow-derived human mesenchymal stem cells (hMSCs) lack the Coxsackie-adenovirus (Ad) receptor and thus are poorly transduced by vectors based on human Ad serotype 5 (Ad5). We investigated whether this problem could be overcome by using tropism-modified Ad5 vectors carrying fiber shaft domains and knobs of different human species B Ads (Ad5FBs). To allow quantitative analyses, these vectors coded for the enhanced green fluorescent protein (eGFP). Transgene expression analysis showed superi... Abstract
Cited 66 times since 2005 (3.6 per year) source: EuropePMC
Journal of the American College of Cardiology, Volume 46, Issue 10, 21 3 2005, Pages 1943-1952 Human adult bone marrow mesenchymal stem cells repair experimental conduction block in rat cardiomyocyte cultures. Beeres SL, Atsma DE, van der Laarse A, Pijnappels DA, van Tuyn J, Fibbe WE, de Vries AA, Ypey DL, van der Wall EE, Schalij MJ
Objectives: We evaluated whether human adult bone marrow-derived mesenchymal stem cells (hMSCs) could repair an experimentally induced conduction block in cardiomyocyte cultures. Background: Autologous stem cell therapy is a novel treatment option for patients with heart disease. However, detailed electrophysiological characterization of hMSCs is still lacking. Methods: Neonatal rat cardiomyocytes were seeded on multi-electrode arrays. After 48 h, abrasion of a 200- to 450-microm-wide channel ca... Abstract
Cited 62 times since 2005 (3.3 per year) source: EuropePMC
Cardiovascular research, Volume 67, Issue 2, 1 1 2005, Pages 245-255 Activation of cardiac and smooth muscle-specific genes in primary human cells after forced expression of human myocardin. van Tuyn J, Knaän-Shanzer S, van de Watering MJ, de Graaf M, van der Laarse A, Schalij MJ, van der Wall EE, de Vries AA, Atsma DE
Objective: Myocardin is a recently discovered transcriptional regulator of cardiac and smooth muscle development. Its ability to transactivate smooth muscle-specific genes has been firmly established in animal cells but its effect on heart muscle genes has been investigated less extensively and the consequences of ectopic myocardin expression in human cells are unknown. Methods: In this study, primary human mesenchymal stem cells and foreskin fibroblasts were transduced with human adenovirus vec... Abstract
Cited 26 times since 2005 (1.4 per year) source: EuropePMC
Journal of virology, Volume 79, Issue 5, 1 1 2005, Pages 3146-3162 Transfer of the full-length dystrophin-coding sequence into muscle cells by a dual high-capacity hybrid viral vector with site-specific integration ability. Gonçalves MA, van Nierop GP, Tijssen MR, Lefesvre P, Knaän-Shanzer S, van der Velde I, van Bekkum DW, Valerio D, de Vries AA
Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene, making it a potential target for gene therapy. There is, however, a scarcity of vectors that can accommodate the 14-kb DMD cDNA and permanently genetically correct muscle tissue in vivo or proliferating myogenic progenitors in vitro for use in autologous transplantation. Here, a dual high-capacity adenovirus-adeno-associated virus (hcAd/AAV) vector with two full-length human dystrophin-coding sequences flanked by AAV integ... Abstract
Cited 56 times since 2004 (2.9 per year) source: EuropePMC
Journal of virology, Volume 78, Issue 23, 1 1 2004, Pages 13019-13027 Structural protein requirements in equine arteritis virus assembly. Wieringa R, de Vries AA, van der Meulen J, Godeke GJ, Onderwater JJ, van Tol H, Koerten HK, Mommaas AM, Snijder EJ, Rottier PJ
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae of the order Nidovirales. EAV particles contain seven structural proteins: the nucleocapsid protein N, the unglycosylated envelope proteins M and E, and the N-glycosylated membrane proteins GP(2b) (previously named G(S)), GP(3), GP(4), and GP(5) (previously named G(L)). Proteins N, M, and GP(5) are major virion components, E occurs in virus particles in intermediate amounts, and GP(4),... Abstract