Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
110 results
Cited 13 times since 2016 (1.6 per year) source: EuropePMC
Scientific reports, Volume 6, 13 2 2016, Pages 24334 Islands of spatially discordant APD alternans underlie arrhythmogenesis by promoting electrotonic dyssynchrony in models of fibrotic rat ventricular myocardium. Majumder R, Engels MC, de Vries AA, Panfilov AV, Pijnappels DA
Fibrosis and altered gap junctional coupling are key features of ventricular remodelling and are associated with abnormal electrical impulse generation and propagation. Such abnormalities predispose to reentrant electrical activity in the heart. In the absence of tissue heterogeneity, high-frequency impulse generation can also induce dynamic electrical instabilities leading to reentrant arrhythmias. However, because of the complexity and stochastic nature of such arrhythmias, the combined effect... Abstract
Cited 15 times since 2016 (1.9 per year) source: EuropePMC
Circulation. Arrhythmia and electrophysiology, Volume 9, Issue 4, 1 1 2016, Pages e003439 Allosteric Modulation of Kv11.1 (hERG) Channels Protects Against Drug-Induced Ventricular Arrhythmias. Yu Z, Liu J, van Veldhoven JP, IJzerman AP, Schalij MJ, Pijnappels DA, Heitman LH, de Vries AA
Background: Ventricular arrhythmias as a result of unintentional blockade of the Kv11.1 (hERG [human ether-à-go-go-related gene]) channel are a major safety concern in drug development. In past years, several highly prescribed drugs have been withdrawn for their ability to cause such proarrhythmia. Here, we investigated whether the proarrhythmic risk of existing drugs could be reduced by Kv11.1 allosteric modulators. Methods and results: Using [(3)H]dofetilide-binding assays with membranes of hu... Abstract
Cited 9 times since 2016 (1.1 per year) source: EuropePMC
Methods in molecular biology (Clifton, N.J.), Volume 1408, 1 1 2016, Pages 319-331 Optogenetic Engineering of Atrial Cardiomyocytes. Feola I, Teplenin A, de Vries AA, Pijnappels DA
Optogenetics is emerging in the cardiology field as a new strategy to explore biological functions through the use of light-sensitive proteins and dedicated light sources. For example, this technology allows modification of the electrophysiological properties of cardiac muscle cells with superb spatiotemporal resolution and quantitative control. In this chapter, the optogenetic modification of atrial cardiomyocytes (aCMCs) from 2-day-old Wistar rats using lentiviral vector (LV) technology and th... Abstract
Cited 6 times since 2015 (0.7 per year) source: EuropePMC
Scientific reports, Volume 5, 21 3 2015, Pages 15187 Constitutively Active Acetylcholine-Dependent Potassium Current Increases Atrial Defibrillation Threshold by Favoring Post-Shock Re-Initiation. Bingen BO, Askar SF, Neshati Z, Feola I, Panfilov AV, de Vries AA, Pijnappels DA
Electrical cardioversion (ECV), a mainstay in atrial fibrillation (AF) treatment, is unsuccessful in up to 10-20% of patients. An important aspect of the remodeling process caused by AF is the constitutive activition of the atrium-specific acetylcholine-dependent potassium current (IK,ACh → IK,ACh-c), which is associated with ECV failure. This study investigated the role of IK,ACh-c in ECV failure and setting the atrial defibrillation threshold (aDFT) in optically mapped neonatal rat cardiomyocy... Abstract
Cited 3 times since 2015 (0.3 per year) source: EuropePMC
Cardiovascular research, Volume 107, Issue 4, 3 1 2015, Pages 601-612 Forced fusion of human ventricular scar cells with cardiomyocytes suppresses arrhythmogenicity in a co-culture model. Engels MC, Askar SF, Jangsangthong W, Bingen BO, Feola I, Liu J, Majumder R, Versteegh MI, Braun J, Klautz RJ, Ypey DL, De Vries AA, Pijnappels DA
Aims: Fibrosis increases arrhythmogenicity in myocardial tissue by causing structural and functional disruptions in the cardiac syncytium. Forced fusion of fibroblastic cells with adjacent cardiomyocytes may theoretically resolve these disruptions. Therefore, the electrophysiological effects of such electrical and structural integration of fibroblastic cells into a cardiac syncytium were studied. Methods and results: Human ventricular scar cells (hVSCs) were transduced with lentiviral vectors en... Abstract
Cited 1 times since 2015 (0.1 per year) source: EuropePMC
World journal of experimental medicine, Volume 5, Issue 2, 20 3 2015, Pages 140-153 Barriers in contribution of human mesenchymal stem cells to murine muscle regeneration. de la Garza-Rodea AS, Boersma H, Dambrot C, de Vries AA, van Bekkum DW, Knaän-Shanzer S
Aim: To study regeneration of damaged human and murine muscle implants and the contribution of added xenogeneic mesenchymal stem cells (MSCs). Methods: Minced human or mouse skeletal muscle tissues were implanted together with human or mouse MSCs subcutaneously on the back of non-obese diabetic/severe combined immunodeficient mice. The muscle tissues (both human and murine) were minced with scalpels into small pieces (< 1 mm(3)) and aliquoted in portions of 200 mm(3). These portions were eith... Abstract
Cited 8 times since 2015 (0.9 per year) source: EuropePMC
Cell transplantation, Volume 24, Issue 12, 23 4 2015, Pages 2463-2478 Human Placenta-Derived Multipotent Cells (hPDMCs) Modulate Cardiac Injury: From Bench to Small and Large Animal Myocardial Ischemia Studies. Liu YH, Peng KY, Chiu YW, Ho YL, Wang YH, Shun CT, Huang SY, Lin YS, de Vries AA, Pijnappels DA, Lee NT, Yen BL, Yen ML
Cardiovascular disease is the leading cause of death globally, and stem cell therapy remains one of the most promising strategies for regeneration or repair of the damaged heart. We report that human placenta-derived multipotent cells (hPDMCs) can modulate cardiac injury in small and large animal models of myocardial ischemia (MI) and elucidate the mechanisms involved. We found that hPDMCs can undergo in vitro cardiomyogenic differentiation when cocultured with mouse neonatal cardiomyocytes. Mor... Abstract
Cited 2 times since 2014 (0.2 per year) source: EuropePMC
Circulation research, Volume 115, Issue 4, 1 1 2014, Pages e6-7 Cardiac anisotropy, regeneration, and rhythm. Pijnappels DA, Schalij MJ, Atsma DE, de Vries AA
Cited 68 times since 2014 (7 per year) source: EuropePMC
Cardiovascular research, Volume 104, Issue 1, 31 5 2014, Pages 194-205 Light-induced termination of spiral wave arrhythmias by optogenetic engineering of atrial cardiomyocytes. Bingen BO, Engels MC, Schalij MJ, Jangsangthong W, Neshati Z, Feola I, Ypey DL, Askar SF, Panfilov AV, Pijnappels DA, de Vries AA
Aims: Atrial fibrillation (AF) is the most common cardiac arrhythmia and often involves reentrant electrical activation (e.g. spiral waves). Drug therapy for AF can have serious side effects including proarrhythmia, while electrical shock therapy is associated with discomfort and tissue damage. Hypothetically, forced expression and subsequent activation of light-gated cation channels in cardiomyocytes might deliver a depolarizing force sufficient for defibrillation, thereby circumventing the afo... Abstract
Cited 1 times since 2014 (0.1 per year) source: EuropePMC
PloS one, Volume 9, Issue 7, 16 3 2014, Pages e102433 Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity. Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA
Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecu... Abstract
Cited 30 times since 2014 (3 per year) source: EuropePMC
Stem cells (Dayton, Ohio), Volume 32, Issue 6, 1 1 2014, Pages 1493-1502 Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm. Engels MC, Rajarajan K, Feistritzer R, Sharma A, Nielsen UB, Schalij MJ, de Vries AA, Pijnappels DA, Wu SM
A thorough understanding of the developmental signals that direct pluripotent stem cells (PSCs) toward a cardiac fate is essential for translational applications in disease modeling and therapy. We screened a panel of 44 cytokines/signaling molecules for their ability to enhance Nkx2.5(+) cardiac progenitor cell (CPC) formation during in vitro embryonic stem cell (ESC) differentiation. Treatment of murine ESCs with insulin or insulin-like growth factors (IGF1/2) during early differentiation incr... Abstract
Cited 58 times since 2014 (5.7 per year) source: EuropePMC
Journal of the American Society of Nephrology : JASN, Volume 25, Issue 8, 7 1 2014, Pages 1710-1722 Hematopoietic microRNA-126 protects against renal ischemia/reperfusion injury by promoting vascular integrity. Bijkerk R, van Solingen C, de Boer HC, van der Pol P, Khairoun M, de Bruin RG, van Oeveren-Rietdijk AM, Lievers E, Schlagwein N, van Gijlswijk DJ, Roeten MK, Neshati Z, de Vries AA, Rodijk M, Pike-Overzet K, van den Berg YW, van der Veer EP, Versteeg HH, Reinders ME, Staal FJ, van Kooten C, Rabelink TJ, van Zonneveld AJ
Ischemia/reperfusion injury (IRI) is a central phenomenon in kidney transplantation and AKI. Integrity of the renal peritubular capillary network is an important limiting factor in the recovery from IRI. MicroRNA-126 (miR-126) facilitates vascular regeneration by functioning as an angiomiR and by modulating mobilization of hematopoietic stem/progenitor cells. We hypothesized that overexpression of miR-126 in the hematopoietic compartment could protect the kidney against IRI via preservation of m... Abstract
Cited 14 times since 2014 (1.4 per year) source: EuropePMC
Cardiovascular research, Volume 102, Issue 2, 27 4 2014, Pages 224-231 Interaction between myofibroblasts and stem cells in the fibrotic heart: balancing between deterioration and regeneration. Ramkisoensing AA, de Vries AA, Atsma DE, Schalij MJ, Pijnappels DA
Signalling between the various cell types in the heart has been investigated for decades. However, relatively little is known about the interplay between the cardiac fibroblasts and myofibroblasts, which help to maintain myocardial tissue structure and function, and resident cardiac or extracardiac stem cells involved in tissue homeostasis and repair. Much of our knowledge about these interactions is derived from experimental animal models, especially those of myocardial infarction and stem cell... Abstract
Cited 25 times since 2013 (2.4 per year) source: EuropePMC
Circulation, Volume 128, Issue 25, 24 4 2013, Pages 2732-2744 Atrium-specific Kir3.x determines inducibility, dynamics, and termination of fibrillation by regulating restitution-driven alternans. Bingen BO, Neshati Z, Askar SF, Kazbanov IV, Ypey DL, Panfilov AV, Schalij MJ, de Vries AA, Pijnappels DA
Background: Atrial fibrillation is the most common cardiac arrhythmia. Ventricular proarrhythmia hinders pharmacological atrial fibrillation treatment. Modulation of atrium-specific Kir3.x channels, which generate a constitutively active current (I(K,ACh-c)) after atrial remodeling, might circumvent this problem. However, it is unknown whether and how I(K,ACh-c) contributes to atrial fibrillation induction, dynamics, and termination. Therefore, we investigated the effects of I(K,ACh-c) blockade... Abstract
Cited 61 times since 2013 (5.7 per year) source: EuropePMC
Circulation research, Volume 113, Issue 9, 20 3 2013, Pages 1065-1075 Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype. van der Veer EP, de Bruin RG, Kraaijeveld AO, de Vries MR, Bot I, Pera T, Segers FM, Trompet S, van Gils JM, Roeten MK, Beckers CM, van Santbrink PJ, Janssen A, van Solingen C, Swildens J, de Boer HC, Peters EA, Bijkerk R, Rousch M, Doop M, Kuiper J, Schalij MJ, van der Wal AC, Richard S, van Berkel TJ, Pickering JG, Hiemstra PS, Goumans MJ, Rabelink TJ, de Vries AA, Quax PH, Jukema JW, Biessen EA, van Zonneveld AJ
Rationale: RNA-binding proteins are critical post-transcriptional regulators of RNA and can influence pre-mRNA splicing, RNA localization, and stability. The RNA-binding protein Quaking (QKI) is essential for embryonic blood vessel development. However, the role of QKI in the adult vasculature, and in particular in vascular smooth muscle cells (VSMCs), is currently unknown. Objective: We sought to determine the role of QKI in regulating adult VSMC function and plasticity. Methods and results: We... Abstract
Cited 1 times since 2013 (0.1 per year) source: EuropePMC
Iranian journal of basic medical sciences, Volume 16, Issue 7, 1 1 2013, Pages 813-821 Generation of Helper Plasmids Encoding Mutant Adeno-associated Virus Type 2 Capsid Proteins with Increased Resistance against Proteasomal Degradation. Ahmadiankia N, Neshati V, Neshati Z, Swildens J, de Vries AA
Objective(s): Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast and simple method is presented to generate AAV2 vector helper plasmids encoding capsid proteins with single, double or triple Y→F mutations. Materials and methods: A one-step, high-... Abstract
Cited 25 times since 2013 (2.2 per year) source: EuropePMC
Circulation. Arrhythmia and electrophysiology, Volume 6, Issue 2, 18 3 2013, Pages 380-391 Engraftment patterns of human adult mesenchymal stem cells expose electrotonic and paracrine proarrhythmic mechanisms in myocardial cell cultures. Askar SF, Ramkisoensing AA, Atsma DE, Schalij MJ, de Vries AA, Pijnappels DA
Background: After intramyocardial injection, mesenchymal stem cells (MSCs) may engraft and influence host myocardium. However, engraftment rate and pattern of distribution are difficult to control in vivo, hampering assessment of potential adverse effects. In this study, the role of the engraftment patterns of MSCs on arrhythmicity in controllable in vitro models is investigated. Methods and results: Cocultures of 4×10(5) neonatal rat cardiomyocytes and 7% or 28% adult human MSCs (hMSCs) in diff... Abstract
Cardiovascular research, Volume 98, Issue 1, 7 1 2013, Pages 156-157 Prolongation of minimal action potential duration in sustained fibrillation decreases complexity by transient destabilization: reply. Bingen BO, Askar SF, Schalij MJ, de Vries AA, Pijnappels DA
Cited 17 times since 2013 (1.5 per year) source: EuropePMC
The journal of gene medicine, Volume 15, Issue 1, 1 1 2013, Pages 1-11 Development of an AdEasy-based system to produce first- and second-generation adenoviral vectors with tropism for CAR- or CD46-positive cells. Janssen JM, Liu J, Skokan J, Gonçalves MA, de Vries AA
Background: The AdEasy system has acquired preeminence amongst the various methods for producing first-generation, early region 1 (E1)-deleted human adenovirus (HAdV) vectors (AdVs) as a result of the fast and reproducible recovery of full-length AdV genomes via homologous recombination in Escherichia coli. Methods: From the classical AdEasy system, a new production platform was derived to assemble first- and second-generation [i.e. E1- plus early region 2A (E2A)-deleted] AdVs displaying on thei... Abstract
Cited 3 times since 2012 (0.3 per year) source: EuropePMC
Stem cells (Dayton, Ohio), Volume 30, Issue 12, 1 1 2012, Pages 2830-2834 Brief report: Misinterpretation of coculture differentiation experiments by unintended labeling of cardiomyocytes through secondary transduction: delusions and solutions. Ramkisoensing AA, De Vries AA, Schalij MJ, Atsma DE, Pijnappels DA
Cardiomyogenic differentiation of stem cells can be accomplished by coculture with cardiomyocytes (CMCs). To facilitate their identification, stem cells are often labeled through viral transduction with a fluorescent protein. A second marker to distinguish stem cell-derived CMCs from native CMCs is rarely used. This study aimed to investigate the occurrence of secondary transduction of unlabeled neonatal rat (nr) CMCs after coculture with human cells that had been transduced 0, 7, or 14 days ear... Abstract