Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
110 results
Cited 18 times since 2004 (0.9 per year) source: EuropePMC
Virology, Volume 321, Issue 2, 1 1 2004, Pages 287-296 Stable transduction of large DNA by high-capacity adeno-associated virus/adenovirus hybrid vectors. Gonçalves MA, van der Velde I, Knaän-Shanzer S, Valerio D, de Vries AA
Viral vectors with high cloning capacity and host chromosomal integration ability are in demand for the efficient and permanent genetic modification of target cells with large DNA molecules. We have generated a hybrid gene transfer vehicle consisting of recombinant adeno-associated virus (AAV) replicative intermediates packaged in adenovirus (Ad) capsids. This arrangement allows cell cycle-independent nuclear delivery of recombinant AAV genomes with lengths considerably above the maximum size (i... Abstract
Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation, Volume 12, Issue 1, 1 1 2004, Pages 13-17 Genetic programme of cardiogenesis: implications for therapeutic application. van Tuyn J, de Vries AA, van der Laarse A, Schalij MJ, van der Wall EE, Atsma DE
It has become accepted that new cardiomyocytes can be derived from stem cells. Although the potential for therapeutic application is evident, the reported efficiency of differentiation varies greatly from 0.02 to 54%. To obtain clinically relevant numbers of newly differentiated cardiac cells, stem cell differentiation should be as efficient as possible. A plausible way to increase the efficiency of differentiation of stem cells into cardiomyocytes is through the introduction of cardiac specific... Abstract
Cited 27 times since 2003 (1.3 per year) source: EuropePMC
Journal of virology, Volume 77, Issue 24, 1 1 2003, Pages 12996-13004 Intra- and intermolecular disulfide bonds of the GP2b glycoprotein of equine arteritis virus: relevance for virus assembly and infectivity. Wieringa R, De Vries AA, Post SM, Rottier PJ
Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus belonging to the family Arteriviridae of the order NIDOVIRALES: EAV virions contain six different envelope proteins. The glycoprotein GP(5) (previously named G(L)) and the unglycosylated membrane protein M are the major envelope proteins, while the glycoproteins GP(2b) (previously named G(S)), GP(3), and GP(4) are minor structural proteins. The unglycosylated small hydrophobic envelope protein E is present in virus particles... Abstract
Cited 19 times since 2003 (0.9 per year) source: EuropePMC
Journal of virology, Volume 77, Issue 15, 1 1 2003, Pages 8470-8480 Generation of a candidate live marker vaccine for equine arteritis virus by deletion of the major virus neutralization domain. Castillo-Olivares J, Wieringa R, Bakonyi T, de Vries AA, Davis-Poynter NJ, Rottier PJ
Equine arteritis virus (EAV) is an enveloped plus-strand RNA virus of the family Arteriviridae (order Nidovirales) that causes respiratory and reproductive disease in equids. Protective, virus-neutralizing antibodies (VNAb) elicited by infection are directed predominantly against an immunodominant region in the membrane-proximal domain of the viral envelope glycoprotein G(L), allowing recently the establishment of a sensitive peptide enzyme-linked immunosorbent assay (ELISA) based on this partic... Abstract
Cited 38 times since 2003 (1.8 per year) source: EuropePMC
Journal of virology, Volume 77, Issue 11, 1 1 2003, Pages 6216-6226 Formation of disulfide-linked complexes between the three minor envelope glycoproteins (GP2b, GP3, and GP4) of equine arteritis virus. Wieringa R, de Vries AA, Rottier PJ
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae of the order NIDOVIRALES: Six transmembrane proteins have been identified in EAV particles: the nonglycosylated membrane protein M and the glycoprotein GP(5) (previously named G(L)), which occur as disulfide-bonded heterodimers and are the major viral envelope proteins; the unglycosylated small envelope protein E; and the minor glycoproteins GP(2b) (formerly designated G(S)), GP(3), an... Abstract
Cited 33 times since 2002 (1.5 per year) source: EuropePMC
Journal of virology, Volume 76, Issue 21, 1 1 2002, Pages 10829-10840 Characterization of two new structural glycoproteins, GP(3) and GP(4), of equine arteritis virus. Wieringa R, de Vries AA, Raamsman MJ, Rottier PJ
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae of the order Nidovirales. Four envelope proteins have hitherto been identified in EAV particles: the predominant membrane proteins M and G(L), the unglycosylated small envelope protein E, and the nonabundant membrane glycoprotein G(S). In this study, we established that the products of EAV open reading frame 3 (ORF3) and ORF4 (designated GP(3) and GP(4), respectively) are also minor st... Abstract
Cited 18 times since 2002 (0.8 per year) source: EuropePMC
Journal of virology, Volume 76, Issue 21, 1 1 2002, Pages 10734-10744 Efficient generation and amplification of high-capacity adeno-associated virus/adenovirus hybrid vectors. Gonçalves MA, van der Velde I, Janssen JM, Maassen BT, Heemskerk EH, Opstelten DJ, Knaän-Shanzer S, Valerio D, de Vries AA
Effective gene therapy is dependent on safe gene delivery vehicles that can achieve efficient transduction and sustained transgene expression. We are developing a hybrid viral vector system that combines in a single particle the large cloning capacity and efficient cell cycle-independent nuclear gene delivery of adenovirus (Ad) vectors with the long-term transgene expression and lack of viral genes of adeno-associated virus (AAV) vectors. The strategy being pursued relies on coupling the AAV DNA... Abstract
Cited 47 times since 2001 (2.1 per year) source: EuropePMC
Human gene therapy, Volume 12, Issue 16, 1 1 2001, Pages 1989-2005 Highly efficient targeted transduction of undifferentiated human hematopoietic cells by adenoviral vectors displaying fiber knobs of subgroup B. Knaän-Shanzer S, Van Der Velde I, Havenga MJ, Lemckert AA, De Vries AA, Valerio D
Human hematopoietic stem cells (HSCs) are poorly transduced by vectors based on adenovirus serotype 5 (Ad5). This is primarily due to the paucity of the coxsackievirus-Ad receptor on these cells. In an attempt to change the tropism of Ad5, we constructed a series of chimeric E1-deleted Ad5 vectors in which the shaft and knob of the capsid fibers were exchanged with those of other Ad serotypes. In all these vectors, the Ad E1 region was replaced by an expression cassette containing the cytomegalo... Abstract
Cited 22 times since 2001 (1 per year) source: EuropePMC
Virology, Volume 288, Issue 2, 1 1 2001, Pages 236-246 Generation of a high-capacity hybrid vector: packaging of recombinant adenoassociated virus replicative intermediates in adenovirus capsids overcomes the limited cloning capacity of adenoassociated virus vectors. Gonçalves MA, Pau MG, de Vries AA, Valerio D
Gene therapy aims to complement or, ideally, correct defective genes. The broad clinical application of this emerging technology requires the development of safe high-capacity gene delivery vehicles that combine efficient transduction of dividing as well as quiescent cells with sustained transgene expression. Here we present a new hybrid vector system that unites favorable attributes of adenoassociated virus (AAV) and adenovirus (Ad) vectors in a single particle. This was achieved by inclusion o... Abstract
Cited 19 times since 2001 (0.8 per year) source: EuropePMC
Virology, Volume 284, Issue 2, 1 1 2001, Pages 259-276 Recombinant equine arteritis virus as an expression vector. de Vries AA, Glaser AL, Raamsman MJ, Rottier PJ
Equine arteritis virus (EAV) is the prototypic member of the family Arteriviridae, which together with the Corona- and Toroviridae constitutes the order Nidovirales. A common trait of these positive-stranded RNA viruses is the 3'-coterminal nested set of six to eight leader-containing subgenomic mRNAs which are generated by a discontinuous transcription mechanism and from which the viral open reading frames downstream of the polymerase gene are expressed. In this study, we investigated whet... Abstract
Cited 17 times since 2000 (0.7 per year) source: EuropePMC
Journal of clinical microbiology, Volume 38, Issue 6, 1 1 2000, Pages 2065-2075 Monoclonal antibodies directed against conserved epitopes on the nucleocapsid protein and the major envelope glycoprotein of equine arteritis virus. Weiland E, Bolz S, Weiland F, Herbst W, Raamsman MJ, Rottier PJ, De Vries AA
We recently developed a highly effective immunization procedure for the generation of monoclonal antibodies (MAbs) directed against the porcine reproductive and respiratory syndrome virus (E. Weiland, M. Wieczorek-Krohmer, D. Kohl, K. K. Conzelmann, and F. Weiland, Vet. Microbiol. 66:171-186, 1999). The same method was used to produce a panel of 16 MAbs specific for the equine arteritis virus (EAV). Ten MAbs were directed against the EAV nucleocapsid (N) protein, and five MAbs recognized the maj... Abstract
Cited 33 times since 2000 (1.4 per year) source: EuropePMC
Virology, Volume 270, Issue 1, 1 1 2000, Pages 84-97 Genetic manipulation of equine arteritis virus using full-length cDNA clones: separation of overlapping genes and expression of a foreign epitope. de Vries AA, Glaser AL, Raamsman MJ, de Haan CA, Sarnataro S, Godeke GJ, Rottier PJ
Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae of the order Nidovirales. The unsegmented, infectious genome of EAV is 12,704 nt in length [exclusive of the poly(A) tail] and contains eight overlapping genes that are expressed from a 3'-coterminal nested set of seven leader-containing mRNAs. To investigate the importance of the overlapping gene arrangement in the viral life-cycle and to facilitate the genetic manipulation of th... Abstract
Cited 114 times since 2000 (4.7 per year) source: EuropePMC
Journal of virology, Volume 74, Issue 5, 1 1 2000, Pages 2333-2342 Characterization of the coronavirus mouse hepatitis virus strain A59 small membrane protein E. Raamsman MJ, Locker JK, de Hooge A, de Vries AA, Griffiths G, Vennema H, Rottier PJ
The small envelope (E) protein has recently been shown to play an essential role in the assembly of coronaviruses. Expression studies revealed that for formation of the viral envelope, actually only the E protein and the membrane (M) protein are required. Since little is known about this generally low-abundance virion component, we have characterized the E protein of mouse hepatitis virus strain A59 (MHV-A59), an 83-residue polypeptide. Using an antiserum to the hydrophilic carboxy terminus of t... Abstract
Cited 116 times since 1999 (4.7 per year) source: EuropePMC
Journal of virology, Volume 73, Issue 8, 1 1 1999, Pages 6335-6345 Identification of a novel structural protein of arteriviruses. Snijder EJ, van Tol H, Pedersen KW, Raamsman MJ, de Vries AA
Arteriviruses are positive-stranded RNA viruses with an efficiently organized, polycistronic genome. A short region between the replicase gene and open reading frame (ORF) 2 of the equine arteritis virus (EAV) genome was previously assumed to be untranslated. However, here we report that this segment of the EAV genome contains the 5' part of a novel gene (ORF 2a) which is conserved in all arteriviruses. The 3' part of EAV ORF 2a overlaps with the 5' part of the former ORF 2 (now r... Abstract
Cited 37 times since 1998 (1.5 per year) source: EuropePMC
The Journal of biological chemistry, Volume 273, Issue 45, 1 1 1998, Pages 29905-29914 Structural requirements for O-glycosylation of the mouse hepatitis virus membrane protein. de Haan CA, Roestenberg P, de Wit M, de Vries AA, Nilsson T, Vennema H, Rottier PJ
The mouse hepatitis virus (MHV) membrane (M) protein contains only O-linked oligosaccharides. We have used this protein as a model to study the structural requirements for O-glycosylation. We show that MHV M is modified by the addition of a single oligosaccharide side chain at the cluster of 4 hydroxylamino acids present at its extreme amino terminus and identified Thr at position 5 as the functional acceptor site. The hydroxylamino acid cluster, which is quite conserved among O-glycosylated cor... Abstract
Cited 34 times since 1998 (1.3 per year) source: EuropePMC
Journal of virology, Volume 72, Issue 1, 1 1 1998, Pages 862-867 Identification of the leader-body junctions for the viral subgenomic mRNAs and organization of the simian hemorrhagic fever virus genome: evidence for gene duplication during arterivirus evolution. Godeny EK, de Vries AA, Wang XC, Smith SL, de Groot RJ
Simian hemorrhagic fever virus (SHFV) was recently reclassified and assigned to the new virus family Arteriviridae. During replication, arteriviruses produce a 3' coterminal, nested set of subgenomic mRNAs (sgRNAs). These sgRNAs arise by discontinuous transcription, and each contains a 5' leader sequence which is joined to the body of the mRNA through a conserved junction sequence. Only the 5'-most open reading frame (ORF) is believed to be transcribed from each sgRNA. The SHFV ge... Abstract
Cited 16 times since 1997 (0.6 per year) source: EuropePMC
Theriogenology, Volume 47, Issue 6, 1 1 1997, Pages 1275-1295 Equine arteritis virus. Glaser AL, Chirnside ED, Horzinek MC, de Vries AA
Equine arteritis virus (EAV) is a small, enveloped, positive-stranded RNA virus, in the family Arteriviridae , W.H.ich can infect both horses and donkeys. While the majority of EAV infections are asymptomatic, acutely infected animals may develop a wide range of clinical signs, including pyrexia, limb and ventral edema, depression, rhinitis, and conjunctivitis. The virus may cause abortion and has caused mortality in neonates. After natural EAV infection, most horses develop a solid, long-term i... Abstract
Cited 3 times since 1997 (0.1 per year) source: EuropePMC
Tijdschrift voor diergeneeskunde, Volume 122, Issue 1, 1 1 1997, Pages 2-7 [Equine arteritis virus: clinical symptoms and prevention]. Glaser AL, de Vries AA, Rottier PJ, Horzinek MC, Colenbrander B
Sero-epidemiological surveys have revealed that equine arteritis virus (EAV) is prevalent in most European countries. The virus causes sporadic cases of respiratory disease and abortion in horses, the incidence of which has increased in recent years. Mares and geldings eliminate virus after acute infection, but 30% to 60% of stallions become persistently infected. In these animals, EAV is maintained within the reproductive tract and is shed continuously in the semen. Persistent infection with EA... Abstract
Cited 24 times since 1996 (0.9 per year) source: EuropePMC
The veterinary quarterly, Volume 18, Issue 3, 1 1 1996, Pages 95-99 Equine arteritis virus: a review of clinical features and management aspects. Glaser AL, de Vries AA, Rottier PJ, Horzinek MC, Colenbrander B
Sero-epidemiological surveys have revealed that equine arteritis virus (EAV) is prevalent in most European countries. The virus causes sporadic cases of respiratory disease and abortion in horses, the incidence of which has increased in recent years. Mares and geldings eliminate virus after acute infection, but 30% to 60% of stallions become persistently infected. In these animals, EAV is maintained within the reproductive tract and is shed continuously in the semen. Persistent infection with EA... Abstract
Cited 48 times since 1995 (1.7 per year) source: EuropePMC
The Journal of general virology, Volume 76 ( Pt 9), 1 1 1995, Pages 2223-2233 Comparison of equine arteritis virus isolates using neutralizing monoclonal antibodies and identification of sequence changes in GL associated with neutralization resistance. Glaser AL, de Vries AA, Dubovi EJ
Three murine monoclonal antibodies (MAbs) that neutralize equine arteritis virus (EAV) infectivity were identified and characterized. The antibodies, 93B, 74D(B) and 38F, recognized the major envelope glycoprotein (GL) encoded by open reading frame (ORF) 5 in immunoblots and by immunoprecipitation. All three MAbs were used to compare the Bucyrus isolate of EAV and MAb neutralization-resistant (NR) escape mutants with the vaccine virus and 19 independent field isolates of EAV by virus neutralizat... Abstract