Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
110 results
Cited 46 times since 1995 (1.6 per year) source: EuropePMC
Journal of virology, Volume 69, Issue 8, 1 1 1995, Pages 4668-4674 The two major envelope proteins of equine arteritis virus associate into disulfide-linked heterodimers. de Vries AA, Post SM, Raamsman MJ, Horzinek MC, Rottier PJ
In a coimmunoprecipitation assay with monospecific antisera, the two major envelope proteins GL and M of equine arteritis virus were found to occur in heteromeric complexes in virions and infected cells. While the GL protein associated with M rapidly and efficiently, newly synthesized M protein was incorporated into complexes at a slower rate, which implies that it interacts with GL molecules synthesized earlier. Analysis under nonreducing conditions revealed that the GL/M complexes consist of d... Abstract
Cited 41 times since 1995 (1.4 per year) source: EuropePMC
The Journal of general virology, Volume 76 ( Pt 8), 1 1 1995, Pages 1989-1998 Equine arteritis virus-neutralizing antibody in the horse is induced by a determinant on the large envelope glycoprotein GL. Chirnside ED, de Vries AA, Mumford JA, Rottier PJ
Complementary DNAs encoding ORFs 2 to 7 equine arteritis virus (EAV) have been cloned into the expression vector pGEX to produce glutathione-S-transferase fusion proteins. Recombinant proteins were affinity purified and screened in ELISA with equine sera to identify immunoreactive polypeptides. The large envelope glycoprotein (GL) was identified as the most reactive to EAV-positive equine sera and an immuno-dominant epitope was mapped between amino acids 55 and 98 by subcloning and expression. A... Abstract
Cited 30 times since 1995 (1 per year) source: EuropePMC
Journal of virological methods, Volume 54, Issue 1, 1 1 1995, Pages 1-13 Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus. Chirnside ED, Francis PM, de Vries AA, Sinclair R, Mumford JA
A recombinant glutathione-S-transferase fusion protein expressing amino acids 55-98 of equine arteritis virus (EAV) GL (rGL 55-98) was tested in an ELISA for its ability to detect serum antibodies to EAV. Host antibodies induced following EAV infection bound the recombinant antigen by ELISA. The ELISA specificity and sensitivity were determined with a panel of equine sera including postinfection and postvaccination samples. A good correlation existed between EAV neutralizing antibody titers and... Abstract
Cited 27 times since 1995 (0.9 per year) source: EuropePMC
Journal of virology, Volume 69, Issue 6, 1 1 1995, Pages 3441-3448 The small envelope glycoprotein (GS) of equine arteritis virus folds into three distinct monomers and a disulfide-linked dimer. de Vries AA, Raamsman MJ, van Dijk HA, Horzinek MC, Rottier PJ
The small membrane glycoprotein (GS) of equine arteritis virus (EAV) is a minor virion component but is abundantly expressed in EAV-infected cells. In this study, we have analyzed its membrane topology, folding, oligomerization, and intracellular transport. We show that GS is a class I integral membrane protein with one functional N-glycosylation site. Gel electrophoresis under nonreducing conditions revealed that GS occurs in EAV-infected cells in four monomeric conformations and as disulfide-l... Abstract
Cited 60 times since 1995 (2.1 per year) source: EuropePMC
Virology, Volume 207, Issue 2, 1 1 1995, Pages 518-527 Identification of a neutralization site in the major envelope glycoprotein (GL) of equine arteritis virus. Balasuriya UB, Maclachlan NJ, De Vries AA, Rossitto PV, Rottier PJ
A panel of six neutralizing monoclonal antibodies (MAbs), neutralization-resistant variant (escape mutant [EM]) viruses, and individual viral proteins derived from a vaccinia virus expression system were used to identify the neutralizing determinants of equine arteritis virus (EAV). The neutralizing MAbs recognize a single neutralization site on the 29-kDa envelope glycoprotein of EAV (U. B. R. Balasuriya et al., 1993, J. Gen. Virol., 74, 2525-2529). Vaccinia virus recombinants which express eit... Abstract
Cited 42 times since 1994 (1.4 per year) source: EuropePMC
The Journal of general virology, Volume 75 ( Pt 9), 1 1 1994, Pages 2439-2444 Monoclonal antibodies to equine arteritis virus proteins identify the GL protein as a target for virus neutralization. Deregt D, de Vries AA, Raamsman MJ, Elmgren LD, Rottier PJ
Monoclonal antibodies (MAbs) to equine arteritis virus (EAV) proteins were produced and characterized. The protein specificities of eight MAbs were determined definitively by immunoprecipitation of EAV proteins expressed from vaccinia virus recombinants (VVRs). Included were two new VVRs produced for this study, expressing the M and the GL proteins, respectively. Three MAbs were determined to be N-specific and five MAbs recognized the GL protein. One GL-specific MAb, 17F5, of the IgA class, effi... Abstract
Cited 124 times since 1992 (3.9 per year) source: EuropePMC
Journal of virology, Volume 66, Issue 11, 1 1 1992, Pages 6294-6303 Structural proteins of equine arteritis virus. de Vries AA, Chirnside ED, Horzinek MC, Rottier PJ
We have recently shown that the genome of equine arteritis virus (EAV) contains seven open reading frames (ORFs). We now present data on the structural proteins of EAV and the assignment of their respective genes. Virions are composed of a 14-kDa nucleocapsid protein (N) and three membrane proteins designated M, GS, and GL. M is an unglycosylated protein of 16 kDa, and GS and GL are N-glycosylated proteins of 25 and 30 to 42 kDa, respectively. The broad size distribution of GL results from heter... Abstract
Cited 251 times since 1991 (7.6 per year) source: EuropePMC
Journal of virology, Volume 65, Issue 6, 1 1 1991, Pages 2910-2920 Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily. den Boon JA, Snijder EJ, Chirnside ED, de Vries AA, Horzinek MC, Spaan WJ
The nucleotide sequence of the genome of equine arteritis virus (EAV) was determined from a set of overlapping cDNA clones and was found to contain eight open reading frames (ORFs). ORFs 2 through 7 are expressed from six 3'-coterminal subgenomic mRNAs, which are transcribed from the 3'-terminal quarter of the viral genome. A number of these ORFs are predicted to encode structural EAV proteins. The organization and expression of the 3' part of the EAV genome are remarkably similar... Abstract
Cited 73 times since 1990 (2.2 per year) source: EuropePMC
Nucleic acids research, Volume 18, Issue 11, 1 1 1990, Pages 3241-3247 All subgenomic mRNAs of equine arteritis virus contain a common leader sequence. de Vries AA, Chirnside ED, Bredenbeek PJ, Gravestein LA, Horzinek MC, Spaan WJ
During the replication of equine arteritis virus (EAV) six subgenomic mRNAs are synthesized. We present evidence that the viral mRNAs form a 3'-coterminal nested set and contain a common leader sequence of 208 nucleotides which is encoded by the 5'-end of the genome. The leader is joined to the bodies of mRNA 5 and 6 at positions defined by the sequence 5' UCAAC 3'. The part of the leader sequence flanking the UCAAC motif is very similar to the 5'-splice site of the Tetr... Abstract
Cited 10 times since 1979 (0.2 per year) source: EuropePMC
Clinical nuclear medicine, Volume 4, Issue 5, 1 1 1979, Pages 181-190 On the natural history of Plummer's disease. Wiener JD, de Vries AA
Plummer's disease (autonomous goiter) presents a spectrum of forms, raging from solitary autonomous thyroid nodules to numerous small autonomous areas, and from unequivocal to servere hyperthyroidism. Progression is often very slow, but data on long-term follow up are scare, contradictory and limited to solitary nodules. We re-examined 58 untreated patients on one or more occasions. Follow-up time ranged from 1 to 12 years (average 4 years). There were gross clinical or scintigraphic change... Abstract