Publications
Below you can find a list of our published research.
Below you can find a list of our published research.
239 results
Cited 2 times since 2022 (1.2 per year) source: EuropePMC
JAMA dermatology, Volume 158, Issue 9, 1 1 2022, Pages 1048-1056 Dupilumab Drug Survival and Associated Predictors in Patients With Moderate to Severe Atopic Dermatitis: Long-term Results From the Daily Practice BioDay Registry. Spekhorst LS, de Graaf M, Zuithoff NPA, van den Reek JMPA, Kamsteeg M, Boesjes CM, Romeijn GLE, Loman L, Haeck I, Oosting AJ, de Boer-Brand A, Touwslager WRH, Flinterman A, van Lynden-van Nes AMT, Gostynski AH, de Bruin-Weller MS, Schuttelaar ML
Importance: Long-term data on dupilumab drug survival in patients with atopic dermatitis (AD) are scarce. Furthermore, little is known about the factors associated with drug survival of dupilumab in AD. Objective: To describe the drug survival of dupilumab in patients with AD and to identify associated predictors. Design, setting, and participants: This cohort study was based on data from the multicenter prospective daily practice BioDay registry, in which 4 university and 10 nonuniversity hospi... Abstract
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Volume 168, 29 5 2022, Pages 113393 The role of diet in genotoxicity of fecal water derived from IBD patients and healthy controls. Wang S, Godschalk R, Spooren C, de Graaf M, Jonkers D, van Schooten FJ
Certain dietary factors with anti-inflammatory and/or anti-cancer properties would be a promising preventive strategy for inflammatory bowel disease (IBD) patients against developing colitis-associated colorectal cancer (CAC). In this study, fecal water (FW) was obtained from 80 IBD patients and 20 healthy controls (HCs). The comet assay was applied to determine the DNA damage induced by FW, and the protective potential of FW against hydrogen peroxide (H2O2) induced DNA damage in Caco-2 cells. I... Abstract
Cited 2 times since 2022 (1.2 per year) source: EuropePMC
Scientific reports, Volume 12, Issue 1, 22 4 2022, Pages 14275 Profiling of humoral immune responses to norovirus in children across Europe. Villabruna N, Izquierdo-Lara RW, Schapendonk CME, de Bruin E, Chandler F, Thao TTN, Westerhuis BM, van Beek J, Sigfrid L, Giaquinto C, Goossens H, Bielicki JA, Kohns Vasconcelos M, Fraaij PLA, Koopmans MPG, de Graaf M
Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children... Abstract
Cited 3 times since 2022 (1.7 per year) source: EuropePMC
Allergy, Volume 77, Issue 11, 29 5 2022, Pages 3398-3407 Patient-centered dupilumab dosing regimen leads to successful dose reduction in persistently controlled atopic dermatitis. Spekhorst LS, Bakker D, Drylewicz J, Rispens T, Loeff F, Boesjes CM, Thijs J, Romeijn GLE, Loman L, Schuttelaar ML, van Wijk F, de Graaf M, de Bruin-Weller MS
Background: At present, no real-world studies are available on different dupilumab dosing regimens in controlled atopic dermatitis (AD). The aim of this study was to clinically evaluate a patient-centered dupilumab dosing regimen in patients with controlled AD and to relate this to serum drug levels and serum biomarkers. Methods: Ninety adult AD patients from the prospective BioDay registry were included based on their dupilumab administration interval according to a predefined patient-centered... Abstract
The international journal of cardiovascular imaging, Volume 38, Issue 12, 19 3 2022, Pages 2771-2779 Left ventricular assist device and pump thrombosis: the importance of the inflow cannula position. Kortekaas KA, de Graaf MA, Palmen M, Braun J, Mertens BJA, Tops LF, Beeres SLMA
Pump thrombosis is a devastating complication after left ventricular assist device implantation. This study aims to elucidate the relation between left ventricular assist device implantation angle and risk of pump thrombosis. Between November 2010 and March 2020, 53 left ventricular assist device-patients underwent a computed tomography scan. Using a 3-dimensional multiplanar reformation the left ventricular axis was reconstructed to measure the implantation angle of the inflow cannula. All pati... Abstract
Cited 3 times since 2022 (1.7 per year) source: EuropePMC
Journal of the European Academy of Dermatology and Venereology : JEADV, Volume 36, Issue 11, 19 3 2022, Pages 2153-2165 Systemic treatment of children and adolescents with atopic dermatitis aged ≥2 years: a Delphi consensus project mapping expert opinion in Northern Europe. de Graaf M, Janmohamed SR, Schuttelaar MLA, Agner T, Alfonso JH, De Schepper S, Deleuran M, Despontin K, Elenius V, Ghislain PD, Huilaja L, Johansson EK, Kvenshagen BK, Mandelin JM, Olset H, Svensson A, van Tuyll van Serooskerken AM, Thyssen JP, Vestergaard C
Background: Paediatric atopic dermatitis (AD) can be burdensome, affecting mental health and impairing quality of life for children and caregivers. Comprehensive guidelines exist for managing paediatric AD, but practical guidance on using systemic therapy is limited, particularly for new therapies including biologics and Janus kinase (JAK) inhibitors, recently approved for various ages in this indication. Objectives: This expert consensus aimed to provide practical recommendations within this ad... Abstract
The international journal of cardiovascular imaging, Volume 38, Issue 7, 9 2 2022, Pages 1639-1650 Atherosclerotic plaque characteristics on quantitative coronary computed tomography angiography associated with ischemia on positron emission tomography in diabetic patients. Kamperidis V, de Graaf MA, Uusitalo V, Saraste A, Kuneman JH, van den Hoogen IJ, Knuuti J, Bax JJ
Patients with diabetes mellitus (DM) may show diffuse coronary artery atherosclerosis on coronary computed tomography angiography (CTA). The present study aimed at quantification of atherosclerotic plaque with CTA and its association with myocardial ischemia on positron emission tomography (PET) in DM patients. Of 922 symptomatic outpatients without previously known coronary artery disease who underwent CTA, 115 with DM (mean age 65 ± 8 years, 58% male) who had coronary atherosclerosis and under... Abstract
The international journal of cardiovascular imaging, Volume 38, Issue 9, 16 3 2022, Pages 1951-1960 Myocardial function in COVID-19 patients after hospital discharge: a descriptive study comparing the first and second 'wave' patients. Elffers TW, de Graaf MA, Regeer MV, Omara S, Schalij MJ, Groeneveld GH, Roukens AHE, Geelhoed JJM, Antoni ML
In hospitalized COVID-19 patients, myocardial injury and echocardiographic abnormalities have been described. The present study investigates cardiac function in COVID-19 patients 6 weeks post-discharge and evaluates its relation to New York Heart Association (NYHA) class. Furthermore cardiac function post-discharge between the first and second wave COVID-19 patients was compared. We evaluated 146 patients at the outpatient clinic of the Leiden University Medical Centre. NYHA class of II or highe... Abstract
Radiology case reports, Volume 17, Issue 5, 24 4 2022, Pages 1734-1736 Transmural ischemia visualized using routine Chest CTA. van Driest FY, Hertgers O, Scholte AJHA, Jukema JW, de Graaf MA
Chest computed tomography (CT) is not routinely used for the diagnosis of myocardial infarction. However, there have been some reports of patients undergoing chest CT for other indications in which ultimately MI was diagnosed due to the presence of areas of myocardial hypoperfusion. The authors present the case of a 60-years old male who is referred due to acute chest pain radiating between the scapulae. Thoracic computed tomography angiography to rule out an aortic dissection revealed an occlus... Abstract
Cited 4 times since 2022 (1.9 per year) source: EuropePMC
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Volume 52, Issue 6, 18 3 2022, Pages 801-805 Ocular surface disease is common in moderate-to-severe atopic dermatitis patients. Achten RE, Bakker DS, van Luijk CM, van der Wal M, de Graaf M, van Wijk F, Zuithoff NPA, van der Rijst LP, Boesjes CM, Thijs JL, de Boer JH, de Bruin-Weller MS
Cited 1 times since 2022 (0.5 per year) source: EuropePMC
Acta dermato-venereologica, Volume 102, 11 2 2022, Pages adv00666 Identification of Risk Factors for Dupilumab-associated Ocular Surface Disease in Patients with Atopic Dermatitis. Achten RE, Van Luijk C, Van der Rijst L, Bakker D, Spekhorst L, Zuithoff N, Schuttelaar M, Romeijn G, Voorberg A, Kamsteeg M, Haeck I, De Graaf M, Thijs J, De Boer J, De Bruin-Weller M
This study identified risk factors for the development of dupilumab-associated ocular surface disease in patients with moderate-to-severe atopic dermatitis in a large prospective daily practice cohort. Data from the Dutch BioDay Registry were used to assess the risk of developing dupilumab-associated ocular surface disease, by performing univariate and multivariate logistic regression analyses. A total of 469 patients were included, of which 152/469 (32.4%) developed dupilumab-associated ocular... Abstract
Cited 26 times since 2022 (11.7 per year) source: EuropePMC
Water research, Volume 214, 5 1 2022, Pages 118162 Prevalence and circulation patterns of SARS-CoV-2 variants in European sewage mirror clinical data of 54 European cities. Agrawal S, Orschler L, Schubert S, Zachmann K, Heijnen L, Tavazzi S, Gawlik BM, de Graaf M, Medema G, Lackner S
For community-level monitoring, the European Commission under the EU Sewage Sentinel System recommends wastewater-based SARS-CoV-2 surveillance. Tracking SARS-CoV-2 variants in a community is pivotal for appropriate public health response. Genome sequencing of SARS-CoV-2 in wastewater samples for tracking variants is challenging, often resulting in low coverage genome sequences, thereby impeding the detection of the SARS-CoV-2 mutations. Therefore, we aimed at high-coverage SARS-CoV-2 genome seq... Abstract
Trials, Volume 22, Issue 1, 11 2 2021, Pages 902 The effectiveness of antibacterial therapeutic clothing based on silver or chitosan as compared with non-antibacterial therapeutic clothing in patients with moderate to severe atopic dermatitis (ABC trial): study protocol for a pragmatic randomized controlled trial. Ragamin A, Fieten KB, Tupker RA, de Wit J, van Mierlo MMF, Jansen MS, Bronner MB, Schappin R, Schuren FHJ, Romeijn MLE, Arents BWM, Polinder S, de Graaf M, Rustemeyer T, Schuttelaar MLA, Pasmans SGMA
Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease that affects 10 to 20% of children and between 2 and 15% of the adults in Western Europe. Since 2000, therapeutic clothing or functional textiles based on silver or chitosan as antibacterial agents were introduced for AD. These agents aim to reduce skin colonization with Staphylococcus (S.) aureus. Increased colonization with S. aureus is correlated with increased AD severity. The antimicrobial effects of silver and chitos... Abstract
Cited 4 times since 2021 (1.5 per year) source: EuropePMC
Frontiers in immunology, Volume 12, 18 3 2021, Pages 676662 Selective Binding of Heparin/Heparan Sulfate Oligosaccharides to Factor H and Factor H-Related Proteins: Therapeutic Potential for C3 Glomerulopathies. Loeven MA, Maciej-Hulme ML, Yanginlar C, Hubers MC, Kellenbach E, de Graaf M, van Kuppevelt TH, Wetzels J, Rabelink TJ, Smith RJH, van der Vlag J
Complement dysregulation is characteristic of the renal diseases atypical hemolytic uremic syndrome (aHUS) and complement component 3 glomerulopathy (C3G). Complement regulatory protein Factor H (FH) inhibits complement activity, whereas FH-related proteins (FHRs) lack a complement regulatory domain. FH and FHRs compete for binding to host cell glycans, in particular heparan sulfates (HS). HS is a glycosaminoglycan with an immense structural variability, where distinct sulfation patterns mediate... Abstract
Cited 59 times since 2021 (21.7 per year) source: EuropePMC
The Science of the total environment, Volume 799, 4 1 2021, Pages 149456 Droplet digital RT-PCR to detect SARS-CoV-2 signature mutations of variants of concern in wastewater. Heijnen L, Elsinga G, de Graaf M, Molenkamp R, Koopmans MPG, Medema G
Wastewater surveillance has shown to be a valuable and efficient tool to obtain information about the trends of COVID-19 in the community. Since the recent emergence of new variants, associated with increased transmissibility and/or antibody escape (variants of concern), there is an urgent need for methods that enable specific and timely detection and quantification of the occurrence of these variants in the community. In this study, we demonstrate the use of RT-ddPCR on wastewater samples for s... Abstract
Cited 11 times since 2021 (3.9 per year) source: EuropePMC
The Journal of allergy and clinical immunology, Volume 149, Issue 1, 6 1 2021, Pages 125-134 Unraveling heterogeneity in pediatric atopic dermatitis: Identification of serum biomarker based patient clusters. Bakker DS, de Graaf M, Nierkens S, Delemarre EM, Knol E, van Wijk F, de Bruin-Weller MS, Drylewicz J, Thijs JL
Background: Increasing evidence shows that pediatric atopic dermatitis (AD) differs from adult AD on a biologic level. Broad biomarker profiling across a wide range of ages of pediatric patients with AD is lacking. Objective: Our aim was to identify serum biomarker profiles in children with AD aged 0 to 17 years and compare these profiles with those previously found in adults with AD. Methods: Luminex multiplex immunoassays were used to measure 145 biomarkers in serum from 240 children with AD (... Abstract
Cited 2 times since 2021 (0.7 per year) source: EuropePMC
The Journal of dermatological treatment, Volume 33, Issue 4, 21 3 2021, Pages 1986-1989 Eczema control and treatment satisfaction in atopic dermatitis patients treated with dupilumab - a cross-sectional study from the BioDay registry. Oosterhaven JAF, Spekhorst LS, Zhang J, Voorberg AN, Romeijn GLE, Boesjes CM, de Graaf M, de Bruin-Weller MS, Schuttelaar MLA
Background: Eczema control is a new construct to be measured in atopic dermatitis (AD). Objectives: Measuring patient-perceived eczema control and treatment satisfaction in AD patients, treated with dupilumab between 16 and 52 weeks. Methods: Cross-sectional questionnaire study. Patients from the Dutch BioDay registry completed the Atopic Dermatitis Control Test (ADCT), Recap of Atopic Eczema (RECAP) and Treatment Satisfaction Questionnaire for Medication, Version II (TSQM v. II), along with oth... Abstract
Cited 4 times since 2021 (1.4 per year) source: EuropePMC
Journal of critical care, Volume 65, 23 4 2021, Pages 36-41 Reduced quality of life in ICU survivors - the story behind the numbers: A mixed methods study. Geense WW, de Graaf M, Vermeulen H, van der Hoeven J, Zegers M, van den Boogaard M
Purpose: To gain insight into the daily functioning of ICU survivors who reported a reduced quality of life (QoL) one year after ICU admission. Materials and methods: A two-phase mixed method study design. QoL was assessed using the SF-36 questionnaire before admission and after one year (Phase 1). Participants reporting a reduced QoL were invited for an in-depth interview (Phase 2). Interview data were coded thematically using the PROMIS framework. Results: Of the 797 participants, 173 (22%) re... Abstract
Cited 3 times since 2021 (1 per year) source: EuropePMC
Journal of virological methods, Volume 297, 19 3 2021, Pages 114196 A luciferase-based approach for measuring HBGA blockade antibody titers against human norovirus. van Loben Sels JM, Meredith LW, Sosnovtsev SV, de Graaf M, Koopmans MPG, Lindesmith LC, Baric RS, Green KY, Goodfellow IG
Background: Noroviruses are the most common cause of viral gastroenteritis worldwide, yet there is a deficit in the understanding of protective immunity. Surrogate neutralization assays have been widely used that measure the ability of antibodies to block virus-like particle (VLP) binding to histo-blood group antigens (HBGAs). However, screening large sample sets against multiple antigens using the traditional HBGA blocking assay requires significant investment in terms of time, equipment, and t... Abstract
Cited 114 times since 2021 (38.2 per year) source: EuropePMC
Emerging infectious diseases, Volume 27, Issue 5, 1 1 2021, Pages 1405-1415 Monitoring SARS-CoV-2 Circulation and Diversity through Community Wastewater Sequencing, the Netherlands and Belgium. Izquierdo-Lara R, Elsinga G, Heijnen L, Munnink BBO, Schapendonk CME, Nieuwenhuijse D, Kon M, Lu L, Aarestrup FM, Lycett S, Medema G, Koopmans MPG, de Graaf M
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly become a major global health problem, and public health surveillance is crucial to monitor and prevent virus spread. Wastewater-based epidemiology has been proposed as an addition to disease-based surveillance because virus is shed in the feces of ≈40% of infected persons. We used next-generation sequencing of sewage samples to evaluate the diversity of SARS-CoV-2 at the community level in the Netherlands and Belgium. Phylo... Abstract