104 results
Cardiovascular research,
16 3 2021
Optical ventricular cardioversion by local optogenetic targeting and LED implantation in a cardiomyopathic rat model.
Nyns ECA, Jin T, Fontes MS, van den Heuvel T, Portero V, Ramsey C, Bart CI, Zeppenfeld K, Schalij MJ, van Brakel TJ, Ramkisoensing AA, Qi Zhang G, Poelma RH, Ördög B, de Vries AAF, Pijnappels DA
<h4>Aims</h4>Ventricular tachyarrhythmias (VTs) are common in the pathologically remodelled heart. These arrhythmias can be lethal, necessitating acute treatment like electrical cardioversion to restore normal rhythm. Recently, it has been proposed that cardioversion may also be realized via optically controlled generation of bioelectricity by the arrhythmic heart itself through optogenetics and therefore without the need of traumatizing high-voltage shocks. However, crucial mechanis...
Abstract
Frontiers in physiology,
Volume 12, 23 4 2021, Pages 710020
The Effects of Repetitive Use and Pathological Remodeling on Channelrhodopsin Function in Cardiomyocytes.
Ördög B, Teplenin A, De Coster T, Bart CI, Dekker SO, Zhang J, Ypey DL, de Vries AAF, Pijnappels DA
<b>Aim:</b> Channelrhodopsins (ChRs) are a large family of light-gated ion channels with distinct properties, which is of great importance in the selection of a ChR variant for a given application. However, data to guide such selection for cardiac optogenetic applications are lacking. Therefore, we investigated the functioning of different ChR variants in normal and pathological hypertrophic cardiomyocytes subjected to various illumination protocols. <b>Methods and Results:<...
Abstract
Cited 1 times since 2020 (0.7 per year)
source: EuropePMC
eLife,
Volume 9, 8 2 2020
Self-restoration of cardiac excitation rhythm by anti-arrhythmic ion channel gating.
Majumder R, De Coster T, Kudryashova N, Verkerk AO, Kazbanov IV, Ördög B, Harlaar N, Wilders R, de Vries AA, Ypey DL, Panfilov AV, Pijnappels DA
Homeostatic regulation protects organisms against hazardous physiological changes. However, such regulation is limited in certain organs and associated biological processes. For example, the heart fails to self-restore its normal electrical activity once disturbed, as with sustained arrhythmias. Here we present proof-of-concept of a biological self-restoring system that allows automatic detection and correction of such abnormal excitation rhythms. For the heart, its realization involves the inte...
Abstract
Cited 3 times since 2020 (1.9 per year)
source: EuropePMC
Frontiers in cardiovascular medicine,
Volume 7, 31 5 2020, Pages 43
Multicellular In vitro Models of Cardiac Arrhythmias: Focus on Atrial Fibrillation.
van Gorp PRR, Trines SA, Pijnappels DA, de Vries AAF
Atrial fibrillation (AF) is the most common cardiac arrhythmia in clinical practice with a large socioeconomic impact due to its associated morbidity, mortality, reduction in quality of life and health care costs. Currently, antiarrhythmic drug therapy is the first line of treatment for most symptomatic AF patients, despite its limited efficacy, the risk of inducing potentially life-threating ventricular tachyarrhythmias as well as other side effects. Alternative, in-hospital treatment modalitie...
Abstract
Journal of applied physiology (Bethesda, Md. : 1985),
Volume 128, Issue 3, 30 5 2020, Pages 545-553
The proarrhythmic features of pathological cardiac hypertrophy in neonatal rat ventricular cardiomyocyte cultures.
Neshati Z, Schalij MJ, de Vries AAF
Different factors may trigger arrhythmias in diseased hearts, including fibrosis, cardiomyocyte hypertrophy, hypoxia, and inflammation. This makes it difficult to establish the relative contribution of each of them to the occurrence of arrhythmias. Accordingly, in this study, we used an in vitro model of pathological cardiac hypertrophy (PCH) to investigate its proarrhythmic features and the underlying mechanisms independent of fibrosis or other PCH-related processes. Neonatal rat ventricular ca...
Abstract
Cited 16 times since 2019 (5.9 per year)
source: Scopus
Science translational medicine,
Volume 11, Issue 481, 1 1 2019
An automated hybrid bioelectronic system for autogenous restoration of sinus rhythm in atrial fibrillation.
Nyns ECA, Poelma RH, Volkers L, Plomp JJ, Bart CI, Kip AM, van Brakel TJ, Zeppenfeld K, Schalij MJ, Zhang GQ, de Vries AAF, Pijnappels DA
Because of suboptimal therapeutic strategies, restoration of sinus rhythm in symptomatic atrial fibrillation (AF) often requires in-hospital delivery of high-voltage shocks, thereby precluding ambulatory AF termination. Continuous, rapid restoration of sinus rhythm is desired given the recurring and progressive nature of AF. Here, we present an automated hybrid bioelectronic system for shock-free termination of AF that enables the heart to act as an electric current generator for autogenous rest...
Abstract
Cited 9 times since 2018 (3.1 per year)
source: Scopus
Cardiovascular research,
Volume 114, Issue 14, 1 1 2018, Pages 1848-1859
Generation and primary characterization of iAM-1, a versatile new line of conditionally immortalized atrial myocytes with preserved cardiomyogenic differentiation capacity.
Liu J, Volkers L, Jangsangthong W, Bart CI, Engels MC, Zhou G, Schalij MJ, Ypey DL, Pijnappels DA, de Vries AAF
<h4>Aims</h4>The generation of homogeneous cardiomyocyte populations from fresh tissue or stem cells is laborious and costly. A potential solution to this problem would be to establish lines of immortalized cardiomyocytes. However, as proliferation and (terminal) differentiation of cardiomyocytes are mutually exclusive processes, their permanent immortalization causes loss of electrical and mechanical functions. We therefore aimed at developing conditionally immortalized atrial myocy...
Abstract
Cited 5 times since 2018 (1.6 per year)
source: Scopus
eLife,
Volume 7, 27 4 2018
Optogenetics enables real-time spatiotemporal control over spiral wave dynamics in an excitable cardiac system.
Majumder R, Feola I, Teplenin AS, de Vries AA, Panfilov AV, Pijnappels DA
Propagation of non-linear waves is key to the functioning of diverse biological systems. Such waves can organize into spirals, rotating around a core, whose properties determine the overall wave dynamics. Theoretically, manipulation of a spiral wave core should lead to full spatiotemporal control over its dynamics. However, this theory lacks supportive evidence (even at a conceptual level), making it thus a long-standing hypothesis. Here, we propose a new phenomenological concept that involves a...
Abstract
Cited 4 times since 2018 (1.1 per year)
source: Scopus
Journal of cellular biochemistry,
Volume 119, Issue 7, 10 2 2018, Pages 6146-6153
Cardiomyogenic differentiation of human adipose-derived mesenchymal stem cells transduced with Tbx20-encoding lentiviral vectors.
Neshati V, Mollazadeh S, Fazly Bazzaz BS, de Vries AA, Mojarrad M, Naderi-Meshkin H, Neshati Z, Kerachian MA
Ischemic heart disease often results in myocardial infarction and is the leading cause of mortality and morbidity worldwide. Improvement in the function of infarcted myocardium is a main purpose of cardiac regenerative medicine. One possible way to reach this goal is via stem cell therapy. Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types but display limited cardiomyogenic differentiation potential. Members of the T-box family of tran...
Abstract
Circulation. Arrhythmia and electrophysiology,
Volume 11, Issue 2, 1 1 2018, Pages e006130
Response by Feola et al to Letter Regarding Article, "Localized Optogenetic Targeting of Rotors in Atrial Cardiomyocyte Monolayers".
Feola I, Volkers L, Majumder R, Teplenin A, Schalij MJ, Panfilov AV, de Vries AAF, Pijnappels DA
Abstract
Cited 16 times since 2017 (4 per year)
source: EuropePMC
Circulation. Arrhythmia and electrophysiology,
Volume 10, Issue 11, 1 1 2017
Localized Optogenetic Targeting of Rotors in Atrial Cardiomyocyte Monolayers.
Feola I, Volkers L, Majumder R, Teplenin A, Schalij MJ, Panfilov AV, de Vries AAF, Pijnappels DA
<h4>Background</h4>Recently, a new ablation strategy for atrial fibrillation has emerged, which involves the identification of rotors (ie, local drivers) followed by the localized targeting of their core region by ablation. However, this concept has been subject to debate because the mode of arrhythmia termination remains poorly understood, as dedicated models and research tools are lacking. We took a unique optogenetic approach to induce and locally target a rotor in atrial monolaye...
Abstract
Cited 44 times since 2017 (10.2 per year)
source: Scopus
European heart journal,
Volume 38, Issue 27, 1 1 2017, Pages 2132-2136
Optogenetic termination of ventricular arrhythmias in the whole heart: towards biological cardiac rhythm management.
Nyns ECA, Kip A, Bart CI, Plomp JJ, Zeppenfeld K, Schalij MJ, de Vries AAF, Pijnappels DA
<h4>Aims</h4>Current treatments of ventricular arrhythmias rely on modulation of cardiac electrical function through drugs, ablation or electroshocks, which are all non-biological and rather unspecific, irreversible or traumatizing interventions. Optogenetics, however, is a novel, biological technique allowing electrical modulation in a specific, reversible and trauma-free manner using light-gated ion channels. The aim of our study was to investigate optogenetic termination of ventri...
Abstract
Cited 14 times since 2017 (3 per year)
source: Scopus
Cardiovascular research,
Volume 113, Issue 3, 1 1 2017, Pages 354-366
Optogenetic manipulation of anatomical re-entry by light-guided generation of a reversible local conduction block.
Watanabe M, Feola I, Majumder R, Jangsangthong W, Teplenin AS, Ypey DL, Schalij MJ, Zeppenfeld K, de Vries AA, Pijnappels DA
<h4>Aims</h4>Anatomical re-entry is an important mechanism of ventricular tachycardia, characterized by circular electrical propagation in a fixed pathway. It's current investigative and therapeutic approaches are non-biological, rather unspecific (drugs), traumatizing (electrical shocks), or irreversible (ablation). Optogenetics is a new biological technique that allows reversible modulation of electrical function with unmatched spatiotemporal precision using light-gated ion ch...
Abstract
Cited 43 times since 2016 (8.8 per year)
source: Scopus
EMBO molecular medicine,
Volume 8, Issue 12, 1 1 2016, Pages 1390-1408
TECRL, a new life-threatening inherited arrhythmia gene associated with overlapping clinical features of both LQTS and CPVT.
Devalla HD, Gélinas R, Aburawi EH, Beqqali A, Goyette P, Freund C, Chaix MA, Tadros R, Jiang H, Le Béchec A, Monshouwer-Kloots JJ, Zwetsloot T, Kosmidis G, Latour F, Alikashani A, Hoekstra M, Schlaepfer J, Mummery CL, Stevenson B, Kutalik Z, de Vries AA, Rivard L, Wilde AA, Talajic M, Verkerk AO, Al-Gazali L, Rioux JD, Bhuiyan ZA, Passier R
Genetic causes of many familial arrhythmia syndromes remain elusive. In this study, whole-exome sequencing (WES) was carried out on patients from three different families that presented with life-threatening arrhythmias and high risk of sudden cardiac death (SCD). Two French Canadian probands carried identical homozygous rare variant in TECRL gene (p.Arg196Gln), which encodes the trans-2,3-enoyl-CoA reductase-like protein. Both patients had cardiac arrest, stress-induced atrial and ventricular t...
Abstract
Cited 17 times since 2016 (3.1 per year)
source: Scopus
Scientific reports,
Volume 6, 13 2 2016, Pages 24334
Islands of spatially discordant APD alternans underlie arrhythmogenesis by promoting electrotonic dyssynchrony in models of fibrotic rat ventricular myocardium.
Majumder R, Engels MC, de Vries AA, Panfilov AV, Pijnappels DA
Fibrosis and altered gap junctional coupling are key features of ventricular remodelling and are associated with abnormal electrical impulse generation and propagation. Such abnormalities predispose to reentrant electrical activity in the heart. In the absence of tissue heterogeneity, high-frequency impulse generation can also induce dynamic electrical instabilities leading to reentrant arrhythmias. However, because of the complexity and stochastic nature of such arrhythmias, the combined effect...
Abstract
Cited 10 times since 2016 (1.8 per year)
source: EuropePMC
Circulation. Arrhythmia and electrophysiology,
Volume 9, Issue 4, 1 1 2016, Pages e003439
Allosteric Modulation of Kv11.1 (hERG) Channels Protects Against Drug-Induced Ventricular Arrhythmias.
Yu Z, Liu J, van Veldhoven JP, IJzerman AP, Schalij MJ, Pijnappels DA, Heitman LH, de Vries AA
<h4>Background</h4>Ventricular arrhythmias as a result of unintentional blockade of the Kv11.1 (hERG [human ether-à-go-go-related gene]) channel are a major safety concern in drug development. In past years, several highly prescribed drugs have been withdrawn for their ability to cause such proarrhythmia. Here, we investigated whether the proarrhythmic risk of existing drugs could be reduced by Kv11.1 allosteric modulators.<h4>Methods and results</h4>Using [(3)H]dofetilid...
Abstract
Cited 7 times since 2016 (1.2 per year)
source: Scopus
Methods in molecular biology (Clifton, N.J.),
Volume 1408, 1 1 2016, Pages 319-331
Optogenetic Engineering of Atrial Cardiomyocytes.
Feola I, Teplenin A, de Vries AA, Pijnappels DA
Optogenetics is emerging in the cardiology field as a new strategy to explore biological functions through the use of light-sensitive proteins and dedicated light sources. For example, this technology allows modification of the electrophysiological properties of cardiac muscle cells with superb spatiotemporal resolution and quantitative control. In this chapter, the optogenetic modification of atrial cardiomyocytes (aCMCs) from 2-day-old Wistar rats using lentiviral vector (LV) technology and th...
Abstract
Cited 3 times since 2015 (0.5 per year)
source: Scopus
Scientific reports,
Volume 5, 21 3 2015, Pages 15187
Constitutively Active Acetylcholine-Dependent Potassium Current Increases Atrial Defibrillation Threshold by Favoring Post-Shock Re-Initiation.
Bingen BO, Askar SF, Neshati Z, Feola I, Panfilov AV, de Vries AA, Pijnappels DA
Electrical cardioversion (ECV), a mainstay in atrial fibrillation (AF) treatment, is unsuccessful in up to 10-20% of patients. An important aspect of the remodeling process caused by AF is the constitutive activition of the atrium-specific acetylcholine-dependent potassium current (IK,ACh → IK,ACh-c), which is associated with ECV failure. This study investigated the role of IK,ACh-c in ECV failure and setting the atrial defibrillation threshold (aDFT) in optically mapped neonatal rat cardiomyocy...
Abstract
Cited 3 times since 2015 (0.5 per year)
source: Scopus
Cardiovascular research,
Volume 107, Issue 4, 3 1 2015, Pages 601-612
Forced fusion of human ventricular scar cells with cardiomyocytes suppresses arrhythmogenicity in a co-culture model.
Engels MC, Askar SF, Jangsangthong W, Bingen BO, Feola I, Liu J, Majumder R, Versteegh MI, Braun J, Klautz RJ, Ypey DL, De Vries AA, Pijnappels DA
<h4>Aims</h4>Fibrosis increases arrhythmogenicity in myocardial tissue by causing structural and functional disruptions in the cardiac syncytium. Forced fusion of fibroblastic cells with adjacent cardiomyocytes may theoretically resolve these disruptions. Therefore, the electrophysiological effects of such electrical and structural integration of fibroblastic cells into a cardiac syncytium were studied.<h4>Methods and results</h4>Human ventricular scar cells (hVSCs) were...
Abstract
Cited 1 times since 2015 (0.2 per year)
source: EuropePMC
World journal of experimental medicine,
Volume 5, Issue 2, 20 3 2015, Pages 140-153
Barriers in contribution of human mesenchymal stem cells to murine muscle regeneration.
de la Garza-Rodea AS, Boersma H, Dambrot C, de Vries AA, van Bekkum DW, Knaän-Shanzer S
<h4>Aim</h4>To study regeneration of damaged human and murine muscle implants and the contribution of added xenogeneic mesenchymal stem cells (MSCs).<h4>Methods</h4>Minced human or mouse skeletal muscle tissues were implanted together with human or mouse MSCs subcutaneously on the back of non-obese diabetic/severe combined immunodeficient mice. The muscle tissues (both human and murine) were minced with scalpels into small pieces (< 1 mm(3)) and aliquoted in portions o...
Abstract
