Heart Center Leiden B2Bprogram

The Heart Center Leiden is a multi-disciplinary organization within the Leiden University Medical Center (LUMC) offering optimal patient care and treatment by combining high-end technologies with excellent skills and knowledge, driven by sophisticated science.

It is the Heart Center Leiden's mission to provide the patient with today's most advanced and effective modalities for diagnosis, treatment and prognosis, and to contribute to the development and implementation of tomorrow's modalities. For this purpose, the Heart Center Leiden holds several lines of research, covering the entire spectrum of cardiovascular medicine. These research lines involve both basic and clinical research and have a strong interactive focus, thereby utilizing novel insights into pathological mechanisms for progress towards future therapeutic and preventive treatment options.

The Heart Center Leiden Bench to Bedside (B2B) program is at the heart of our research activities and supports the interdisciplinary and integrative nature of our 5 main research themes: 1) Arrhythmias, 2) Atherosclerosis & Genetics, 3) Congenital Heart Disease, 4) Valvular Disease and 5) Ventricular Dysfunction & Heart Failure.

Mission! and R-Mission! Projects ensure maximal clinical and scientific integrity, while our interacting research lines and a biobanking facility serve as basis for a long-term strategy for high-quality translational science. For more detailed information concerning our research themes please go to menu.

Our research is currently supported, among others, by the Dutch Heart Foundation, The Netherlands Organisation for Scientific Research, Interuniversity Cardiology Institute of the Netherlands, The Royal Netherlands Academy of Arts and Sciences and European Commission.

Selected publications of the Heart Center Leiden:


1. Bingen BO, Neshati Z, Askar SF, Kazbanov IV, Ypey DL, Panfilov AV, Schalij MJ, de Vries AA, Pijnappels DA. Atrium-specific Kir3.x determines inducibility, dynamics, and termination of fibrillation by regulating restitution-driven alternans. Circulation. 2013;128:2732-2744.

2. Wijnmaalen AP, Schalij MJ, von der Thusen JH, Klautz RJ, Zeppenfeld K. Early reperfusion during acute myocardial infarction affects ventricular tachycardia characteristics and the chronic electroanatomic and histological substrate. Circulation. 2010;121:1887-1895.

3. Borleffs CJ, van Rees JB, van Welsenes GH, van der Velde ET, van Erven L, Bax JJ, Schalij MJ. Prognostic importance of atrial fibrillation in implantable cardioverter-defibrillator patients. J Am Coll Cardiol. 2010;55:879-885.

4. Kolditz DP, Wijffels MC, Blom NA, van der Laarse A, Hahurij ND, Lie-Venema H, Markwald RR, Poelmann RE, Schalij MJ, Gittenberger-De Groot AC. Epicardium-derived cells in development of annulus fibrosis and persistence of accessory pathways. Circulation. 2008;117:1508-1517.

5. Zeppenfeld K, Schalij MJ, Bartelings MM, Tedrow UB, Koplan BA, Soejima K, Stevenson WG. Catheter ablation of ventricular tachycardia after repair of congenital heart disease: electroanatomic identification of the critical right ventricular isthmus. Circulation. 2007;116:2241-2252.

Atherosclerosis & Genetics:

1. Boogers MJ, Broersen A, van Velzen JE, de Graaf FR, El-Naggar HM, Kitslaar PH, Dijkstra J, Delgado V, Boersma E, de RA, Schuijf JD, Schalij MJ, Reiber JH, Bax JJ, Jukema JW. Automated quantification of coronary plaque with computed tomography: comparison with intravascular ultrasound using a dedicated registration algorithm for fusion-based quantification. Eur Heart J. 2012;33:1007-1016.

2. van Werkhoven JM, Schuijf JD, Gaemperli O, Jukema JW, Boersma E, Wijns W, Stolzmann P, Alkadhi H, Valenta I, Stokkel MP, Kroft LJ, de Roos A, Pundziute G, Scholte A, van der Wall EE, Kaufmann PA, Bax JJ. Prognostic value of multislice computed tomography and gated single-photon emission computed tomography in patients with suspected coronary artery disease. J Am Coll Cardiol. 2009;53:623-632.

3. Pons D, de Vries FR, van den Elsen PJ, Heijmans BT, Quax PH, Jukema JW. Epigenetic histone acetylation modifiers in vascular remodelling: new targets for therapy in cardiovascular disease. Eur Heart J. 2009;30:266-277.

4. van der Hoeven BL, Liem SS, Jukema JW, Suraphakdee N, Putter H, Dijkstra J, Atsma DE, Bootsma M, Zeppenfeld K, Oemrawsingh PV, van der Wall EE, Schalij MJ. Sirolimus-eluting stents versus bare-metal stents in patients with ST-segment elevation myocardial infarction: 9-month angiographic and intravascular ultrasound results and 12-month clinical outcome results from the MISSION! Intervention Study. J Am Coll Cardiol. 2008;51:618-626.

5. Henneman MM, Schuijf JD, Pundziute G, van Werkhoven JM, van der Wall EE, Jukema JW, Bax JJ. Noninvasive evaluation with multislice computed tomography in suspected acute coronary syndrome: plaque morphology on multislice computed tomography versus coronary calcium score. J Am Coll Cardiol. 2008;52:216-222.

Congenital Heart Disease:

1. van der Hulst AE, Delgado V, Blom NA, van de Veire NR, Schalij MJ, Bax JJ, Roest AA, Holman ER. Cardiac resynchronization therapy in paediatric and congenital heart disease patients. Eur Heart J. 2011;32:2236-2246.

2. Jongbloed MR, Vicente-Steijn R, Douglas YL, Wisse LJ, Mori K, Yokota Y, Bartelings MM, Schalij MJ, Mahtab EA, Poelmann RE, Gittenberger-De Groot AC. Expression of Id2 in the second heart field and cardiac defects in Id2 knock-out mice. Dev Dyn. 2011;240:2561-2577.

3. Scherptong RW, Hazekamp MG, Mulder BJ, Wijers O, Swenne CA, van der Wall EE, Schalij MJ, Vliegen HW. Follow-up after pulmonary valve replacement in adults with tetralogy of Fallot: association between QRS duration and outcome. J Am Coll Cardiol. 2010;56:1486-1492.

4. Hahurij ND, Gittenberger-De Groot AC, Kolditz DP, Bokenkamp R, Schalij MJ, Poelmann RE, Blom NA. Accessory atrioventricular myocardial connections in the developing human heart: relevance for perinatal supraventricular tachycardias. Circulation. 2008;117:2850-2858.

5. Kolditz DP, Wijffels MC, Blom NA, van der Laarse A, Markwald RR, Schalij MJ, Gittenberger-De Groot AC. Persistence of functional atrioventricular accessory pathways in postseptated embryonic avian hearts: implications for morphogenesis and functional maturation of the cardiac conduction system. Circulation. 2007;115:17-26.

Valvular Disease:

1. Ewe SH, Muratori M, Delgado V, Pepi M, Tamborini G, Fusini L, Klautz RJ, Gripari P, Bax JJ, Fusari M, Schalij MJ, Marsan NA. Hemodynamic and clinical impact of prosthesis-patient mismatch after transcatheter aortic valve implantation. J Am Coll Cardiol. 2011;58:1910-1918.

2. Ng AC, Yiu KH, Ewe SH, van der Kley F, Bertini M, de WA, de RA, Leung DY, Schuijf JD, Schalij MJ, Bax JJ, Delgado V. Influence of left ventricular geometry and function on aortic annular dimensions as assessed with multi-detector row computed tomography: implications for transcatheter aortic valve implantation. Eur Heart J. 2011;32:2806-2813.

3. Delgado V, Tops LF, van Bommel RJ, van der Kley F, Marsan NA, Klautz RJ, Versteegh MI, Holman ER, Schalij MJ, Bax JJ. Strain analysis in patients with severe aortic stenosis and preserved left ventricular ejection fraction undergoing surgical valve replacement. Eur Heart J. 2009;30:3037-3047.

4. Lie-Venema H, Eralp I, Markwald RR, van den Akker NM, Wijffels MC, Kolditz DP, van der Laarse A, Schalij MJ, Poelmann RE, Bogers AJ, Gittenberger-De Groot AC. Periostin expression by epicardium-derived cells is involved in the development of the atrioventricular valves and fibrous heart skeleton. Differentiation. 2008;76:809-819.

5. Tops LF, van de Veire NR, Schuijf JD, de Roos A, van der Wall EE, Schalij MJ, Bax JJ. Noninvasive evaluation of coronary sinus anatomy and its relation to the mitral valve annulus: implications for percutaneous mitral annuloplasty. Circulation. 2007;115:1426-1432.

Ventricular Dysfunction & Heart Failure:

1. van Bommel RJ, Marsan NA, Delgado V, Borleffs CJ, van Rijnsoever EP, Schalij MJ, Bax JJ. Cardiac resynchronization therapy as a therapeutic option in patients with moderate-severe functional mitral regurgitation and high operative risk. Circulation. 2011;124:912-919.

2. Braun J, Ciarka A, Versteegh MI, Delgado V, Boersma E, Verwey HF, Schalij MJ, Bax JJ, Dion RA, van de Veire NR, Klautz RJ. Cardiac support device, restrictive mitral valve annuloplasty, and optimized medical treatment: a multimodality approach to nonischemic cardiomyopathy. J Thorac Cardiovasc Surg. 2011;142:e93-100.

3. Delgado V, van Bommel RJ, Bertini M, Borleffs CJ, Marsan NA, Arnold CT, Nucifora G, van de Veire NR, Ypenburg C, Boersma E, Holman ER, Schalij MJ, Bax JJ. Relative merits of left ventricular dyssynchrony, left ventricular lead position, and myocardial scar to predict long-term survival of ischemic heart failure patients undergoing cardiac resynchronization therapy. Circulation. 2011;123:70-78.

4. van Ramshorst J, Bax JJ, Beeres SL, Dibbets-Schneider P, Roes SD, Stokkel MP, de Roos A, Fibbe WE, Zwaginga JJ, Boersma E, Schalij MJ, Atsma DE. Intramyocardial bone marrow cell injection for chronic myocardial ischemia: a randomized controlled trial. JAMA. 2009;301:1997-2004.

5. Pijnappels DA, Schalij MJ, Ramkisoensing AA, van Tuyn J, de Vries AA, van der Laarse A, Ypey DL, Atsma DE. Forced alignment of mesenchymal stem cells undergoing cardiomyogenic differentiation affects functional integration with cardiomyocyte cultures. Circ Res. 2008;103:167-176.